Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,1050AG43.6% 47.6 / 13.4 39I441V (ATC→GTC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (5/17);  new base G (17/0);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 5.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTT  >  NC_000913/4456964‑4457247
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tggcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTcc                                                                                                                                             >  2:60485/1‑145 (MQ=255)
        ggcagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAActgct                                                                                                                                <  1:526365/150‑1 (MQ=255)
            gaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATg                                                                                                                           <  1:425652/151‑1 (MQ=255)
                              gTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGATTTGGTGGGGTCCAACAGAAAATGCTGGGTGGGGTGGCGGAGAAGGCgg                                                                                                         >  1:513228/1‑151 (MQ=255)
                                 cAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGaa                                                                                                       <  1:130810/150‑1 (MQ=255)
                                 cAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCgg                                                                                                         >  2:226363/1‑148 (MQ=255)
                                   gCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGcc                                                                                                    <  2:85180/151‑1 (MQ=255)
                                     cTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGAAGAAGgggg                                                                                                         >  1:338052/1‑144 (MQ=255)
                                     cTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGGAGCcg                                                                                                   >  2:244590/1‑150 (MQ=255)
                                       gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgc                                                                                                  <  2:92103/149‑1 (MQ=255)
                                         acacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAg                                                                                              <  2:655748/151‑1 (MQ=255)
                                                     cGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGGTGGGCGGGCGAAGAAGGCGGGAGCCGCGCAGGAATTCGGCCtt                                                                                  >  1:643949/1‑151 (MQ=255)
                                                     cGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCtt                                                                                  <  2:143287/151‑1 (MQ=255)
                                                     cGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTCCATCAGAAAATGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCtt                                                                                  >  2:288090/1‑151 (MQ=255)
                                                           ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCa                                                                             <  2:43561/150‑1 (MQ=255)
                                                           ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCa                                                                             <  1:280768/150‑1 (MQ=255)
                                                           ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGATCCATCAGAAACTGCTGGGTGGGCTGGCGAAAAAGGCGGAAGCCGCGCAGTAATTCGGCCCTTGcc                                                                              >  2:447876/1‑149 (MQ=255)
                                                                cGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGAtata                                                                       <  1:393024/151‑1 (MQ=255)
                                                                      cAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGATGGGTTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTa                                                                    >  2:646987/1‑148 (MQ=255)
                                                                         atatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTa                                                              <  2:156950/151‑1 (MQ=255)
                                                                                 gtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTCCATCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAgggc                                                          >  1:648434/1‑145 (MQ=255)
                                                                                   gAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATaa                                                    <  2:569405/151‑1 (MQ=255)
                                                                                    aaaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAg                                                   <  1:102393/151‑1 (MQ=255)
                                                                                     aaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCCTCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGGAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATaa                                                    >  2:190758/1‑149 (MQ=255)
                                                                                       aCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTTCCTCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCg                                                >  2:260026/1‑151 (MQ=255)
                                                                                        ccGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGATGGGGTGGGGGAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAgggg                                                >  1:600972/1‑148 (MQ=255)
                                                                                         cGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAAGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCCGATATAGTATGCGTAGGCCGGATAAGGCGtt                                              >  1:75608/1‑151 (MQ=255)
                                                                                          gCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGATGGGCCGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGtt                                              >  2:143489/1‑150 (MQ=255)
                                                                                             cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCa                                            <  2:487565/149‑1 (MQ=255)
                                                                                             cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCa                                            <  1:418122/149‑1 (MQ=255)
                                                                                             cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTCCATCAGAAAATGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc                                           >  2:229075/1‑150 (MQ=255)
                                                                                               gCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGGGGGGTCCATCAGAAACTTCTGGATGGGGTGGGGGAGAAGGCGGAAGCCGCGCAGTAATTCGGCCCTAGCCAGATATAGCAATCGGAGGCCGGGTAAGGCGTTTAcac                                         >  1:214093/1‑148 (MQ=255)
                                                                                                    gCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                   >  2:171104/1‑151 (MQ=255)
                                                                                                         cATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGTCCCTCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGGAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                >  1:301650/1‑149 (MQ=255)
                                                                                                          atatTCTGGTGATGAGCAACGGCGGTTTTGGGGGGATCCCTCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGGGGGAGCCGCGCAGTAATTCGGGCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGCTCACGCCGCATCCgg                                >  1:114558/1‑148 (MQ=255)
                                                                                                            atTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGTTCCCGCCGCATCCGGCAttt                           >  1:263837/1‑151 (MQ=255)
                                                                                                            atTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt                           <  1:516425/151‑1 (MQ=255)
                                                                                                                              ggcggTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGc         >  2:89271/1‑151 (MQ=255)
                                                                                                                                      tggtggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCtt  <  2:325302/150‑1 (MQ=255)
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TGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTT  >  NC_000913/4456964‑4457247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑