| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,984,441 | 0 | A | C | 43.2% | 54.6 / 10.8 | 38 | G384G (GGT→GGG) | yqeF | short chain acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (17/4); new base C (0/16); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGA > NC_000913/2984297‑2984583 | tGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg > 1:258007/1‑151 (MQ=255)tGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTg > 2:297365/1‑150 (MQ=255) cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑ccc < 1:5449/151‑1 (MQ=255) agaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCccgc < 2:129752/151‑1 (MQ=255) aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCccgccg < 1:639122/151‑1 (MQ=255) aTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCccgccg > 2:48035/1‑150 (MQ=255) aaaaaTGAGGGACCCAAAAAAATGTATGTCGACTTGCCACCCTTCATTATCCAACAGATTAATGCCGGCAAATATTTAGCGGTAAGGAGAAAATGCAGGGTTTTTCGTCACTTTAAATGGTCAATCCCCCCCCCGGG‑CCCCCGCCGATACa < 1:437009/151‑1 (MQ=255) gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGt > 2:579788/1‑151 (MQ=255) aGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGcc > 1:454394/1‑150 (MQ=255) caaGAAAATTTATGTCGACTTGCCAGCCATCTTTATCCAACAGATAAATGACGGCAATTTTTTCTGGGAAAGGAGAAAATGCAGATTTTTTCGCACCTTTCAATGGTCAATCCCACCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAg < 1:360690/147‑1 (MQ=255) aaaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATAAATGACGGCAGATTTTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTAAATGGTCAATGCCACCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCttt < 2:483857/151‑1 (MQ=255) tgtCGCCTTGCCACCCATCATTATTCAACAGATTAGTGACGGCGGATATTTACCGGAAGGGAGAAAATGCAGCTCTTTTCGCCATGTTAAAGGGTCAAGCCCACGCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg < 2:59597/144‑1 (MQ=255) tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGCCGGCAGATATTTATCGTAAAGGAGAAAATGCAGAGCTATTCGTCTCGTTCAATGTTCAATCCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg < 1:357626/146‑1 (MQ=255) cGATTTGCCAGCCATCATTATTCAACAGATAAATGACGGCAGATTTTTATCGGTAAGGAGGAAAGGCAGAGCTATTCGTCAGGTTCAATGGTAAATGCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc < 1:122391/151‑1 (MQ=255) gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc > 1:637132/1‑150 (MQ=255) agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc > 2:54449/1‑150 (MQ=255) ttattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg > 2:171957/1‑150 (MQ=255) ttCAAAAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTTTTCGTCACGTTAAATGGTCAATGCCACCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGaa < 2:489871/149‑1 (MQ=255) tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAcc > 1:405600/1‑150 (MQ=255) gATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa > 2:370711/1‑150 (MQ=255) gATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa > 2:450924/1‑150 (MQ=255) gCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCgg > 1:225396/1‑151 (MQ=255) aGATTTTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGCCACGTTCAATGGTCAATCCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCgg < 1:248735/149‑1 (MQ=255) tattaagggttAAGGAGGAAATGCAGAACTATTCGTCACTTAAAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa < 2:478163/139‑1 (MQ=255) tttATCGGTAAGGAGAAAAGGCAGAGCTTTTCGTCCCGTCAAATGGTCAATCCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGAtt < 1:57480/141‑1 (MQ=255) aTCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATGCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg < 2:36278/151‑1 (MQ=255) gcgCTATTCGTCACGTTAATTGGTAAATCCCCCCCCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGt < 2:586118/149‑1 (MQ=255) cTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCg > 1:440972/1‑151 (MQ=255) ttttCGTCACGTTCAAGGTTCAAGCCCACCCCCGGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTg < 1:284964/146‑1 (MQ=255) ttcgccttgtgaaaTGGTAAATGCCACCCCTGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAcc < 1:269165/129‑1 (MQ=255) ttCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATc > 2:517429/1‑151 (MQ=255) tCGTCACGTTCAATGGTCAATCCCCCCCCCTGC‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATc < 1:226602/150‑1 (MQ=255) ttaaaTGGGCAATCCCCCCCCCCGGCCCCCCGCCGATACAAAGCTTTGCCGGTCCTTTGC‑GGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGcca < 2:87398/145‑1 (MQ=255) ttCCATGGTCAATGCCACACCCTGG‑CCCCAGCCGCTACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAACAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGCGACCGAGTGCGATGGCGcc > 1:392477/1‑147 (MQ=255) aaTGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg > 1:342845/1‑151 (MQ=255) cAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg > 2:171415/1‑151 (MQ=255) aaTGCCACCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg < 2:417850/150‑1 (MQ=255) tGCCCCCCCCGGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattga < 1:522697/149‑1 (MQ=255) | TGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGG‑CCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGA > NC_000913/2984297‑2984583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |