Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,306,1360AC57.9% 12.4 / 18.2 19G575G (GGT→GGG) deaDATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (5/3);  new base C (0/11);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 4.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-03

AAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCG  >  NC_000913/3305987‑3306268
                                                                                                                                                      |                                                                                                                                    
aaaCGACGACGACCGGTAGAATCATCACGGCGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCAACACGGCCACCTTCACGGCGTTCCGCGCTGAAGTTACGAGCTCCTTCACGTCGGTCGCACCCGAAA‑CCAAGac                                                                                                                                     >  1:535314/1‑151 (MQ=255)
aaaCGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGac                                                                                                                                     >  2:33887/1‑151 (MQ=255)
   cgacgacgaCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGaccac                                                                                                                                  >  2:416656/1‑151 (MQ=255)
    gacgacgacCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGaccacc                                                                                                                                 >  1:618951/1‑151 (MQ=255)
     acgacgacCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCg                                                                                                                                >  2:526441/1‑151 (MQ=255)
                 aGAATCATCACGACGCGGAGCGCGGCCTTCCCGACGTTCGCCGCTAAAACGACGACCACCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCAAGACGTTCGCCAAAGAAA‑ACACGACCACCGCCACGACGCt                                                                                                                      <  1:637177/149‑1 (MQ=255)
                      catcaCGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCCCGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGc                                                                                                                <  1:513683/150‑1 (MQ=255)
                                               acgacgTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGt                                                                                      >  2:61128/1‑151 (MQ=255)
                                                        gccgcTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATg                                                                             >  1:195514/1‑151 (MQ=255)
                                                                              ccccgccccccTCCACGGCGTCCCCCGCTAAAGTTACGCCCCCCTCCACGCCGTCCCCCCCCGAAC‑CCACGACCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGc                                                       <  2:41813/142‑1 (MQ=255)
                                                                                   cccccctccccgcctttCCCCGCTGAATTAACCCCCGCCTCCCCGAGGTCCGCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGt                                                   <  1:157718/135‑1 (MQ=255)
                                                                                    ccccctccccGGCGTCCCCCGCGAAAGTTCCGCCCCCCTCCCCGCCGTCCCCCCCCGAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTgc                                                 <  2:237870/142‑1 (MQ=255)
                                                                                      aCCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTgcgc                                               >  2:559902/1‑151 (MQ=255)
                                                                                              ggcttcccccGCTGAATTTACGCCCCCCTTCACGACTTCCCCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgt                                        <  2:21262/142‑1 (MQ=255)
                                                                                              ggcttcccccGCTGAAGTTCCGCCCGCCTTCACGCCTTCCGCCCCCGAAC‑CCACGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAg                                         <  2:368595/141‑1 (MQ=255)
                                                                                              ggcttcccccGCTGAAGTTCCGCCCGCCTCCACGCCGTCCCCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgt                                        <  2:194841/142‑1 (MQ=255)
                                                                                              ggCGTCCCCCGCTGAAGTTACGCCCCCCTTCACGCCGTTCCCCACCGAAC‑CCACGCCCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAg                                         <  1:28451/149‑1 (MQ=255)
                                                                                               gCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgggt                                      >  2:451569/1‑151 (MQ=255)
                                                                                                    ccccGCTGAATTTACGCCCCCCTCCACG‑CCTTCCCCCCCAAAACCCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTgca                                  <  2:619046/148‑1 (MQ=255)
                                                                                                                      ccccTTCCCGCCGTCCCCCACCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATAcc               <  2:646089/149‑1 (MQ=255)
                                                                                                                        ccTTCCCGACGTTCGCCCCCAAAC‑CCACGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCt              <  2:397877/150‑1 (MQ=255)
                                                                                                                             acgactTCCCCCACCAAAC‑CCACACCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGc        <  2:399741/151‑1 (MQ=255)
                                                                                                                                     cccccccGAAACCCACACCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCg  <  1:457267/145‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                    
AAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCG  >  NC_000913/3305987‑3306268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑