Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,306,139	0	A	C	57.9%	7.4 / 18.7	19	G574G (GGT→GGG)	deaD	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (5/3);  new base C (0/11);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 4.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-03

CGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCG  >  NC_000913/3305990‑3306268                                                                                                                                                       |                                                                                                                                  cgacgacgaCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGaccac                                                                                                                                  >  2:416656/1‑151 (MQ=255)  gacgacgacCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGaccacc                                                                                                                                 >  1:618951/1‑151 (MQ=255)   acgacgacCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCg                                                                                                                                >  2:526441/1‑151 (MQ=255)               aGAATCATCACGACGCGGAGCGCGGCCTTCCCGACGTTCGCCGCTAAAACGACGACCACCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCAAGACGTTCGCCAAAGAAA‑ACACGACCACCGCCACGACGCt                                                                                                                      <  1:637177/149‑1 (MQ=255)                    catcaCGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCCCGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGc                                                                                                                <  1:513683/150‑1 (MQ=255)                                             acgacgTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGt                                                                                      >  2:61128/1‑151 (MQ=255)                                                      gccgcTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATg                                                                             >  1:195514/1‑151 (MQ=255)                                                                            ccccgccccccTCCACGGCGTCCCCCGCTAAAGTTACGCCCCCCTCCACGCCGTCCCCCCCCGAAC‑CCACGACCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGc                                                       <  2:41813/142‑1 (MQ=255)                                                                                 cccccctccccgcctttCCCCGCTGAATTAACCCCCGCCTCCCCGAGGTCCGCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGt                                                   <  1:157718/135‑1 (MQ=255)                                                                                  ccccctccccGGCGTCCCCCGCGAAAGTTCCGCCCCCCTCCCCGCCGTCCCCCCCCGAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTgc                                                 <  2:237870/142‑1 (MQ=255)                                                                                    aCCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTgcgc                                               >  2:559902/1‑151 (MQ=255)                                                                                            ggcttcccccGCTGAATTTACGCCCCCCTTCACGACTTCCCCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgt                                        <  2:21262/142‑1 (MQ=255)                                                                                            ggcttcccccGCTGAAGTTCCGCCCGCCTTCACGCCTTCCGCCCCCGAAC‑CCACGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAg                                         <  2:368595/141‑1 (MQ=255)                                                                                            ggcttcccccGCTGAAGTTCCGCCCGCCTCCACGCCGTCCCCCCCCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgt                                        <  2:194841/142‑1 (MQ=255)                                                                                            ggCGTCCCCCGCTGAAGTTACGCCCCCCTTCACGCCGTTCCCCACCGAAC‑CCACGCCCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAg                                         <  1:28451/149‑1 (MQ=255)                                                                                             gCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAgggt                                      >  2:451569/1‑151 (MQ=255)                                                                                                  ccccGCTGAATTTACGCCCCCCTCCACG‑CCTTCCCCCCCAAAACCCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTgca                                  <  2:619046/148‑1 (MQ=255)                                                                                                                    ccccTTCCCGCCGTCCCCCACCAAAC‑CCCCGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATAcc               <  2:646089/149‑1 (MQ=255)                                                                                                                      ccTTCCCGACGTTCGCCCCCAAAC‑CCACGCCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCt              <  2:397877/150‑1 (MQ=255)                                                                                                                           acgactTCCCCCACCAAAC‑CCACACCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGc        <  2:399741/151‑1 (MQ=255)                                                                                                                                   cccccccGAAACCCACACCCCCCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCg  <  1:457267/145‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                  CGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGACGTTCGCCACCGAAA‑CCACGACCACCGCCACGACGCTCACCGCCAGTATGCGGCTGTGCATCGCCCAGTAACTGCATGTTCATCGGCTTGTTGAGAATGCGAGTGCGCGTAAAGTGTTGCAGCACTTCACCCGGCATACCTTTCGGCAGTTCG  >  NC_000913/3305990‑3306268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑