Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,412,8980CT46.3% 22.6 / 36.2 41A190T (GCC→ACC) acrSacrAB operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (17/5);  new base T (0/19);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 3.15e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

CCACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCG  >  NC_000913/3412750‑3413045
                                                                                                                                                    |                                                                                                                                                   
ccACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCtt                                                                                                                                                   <  2:183879/151‑1 (MQ=255)
 cagtAAAACTGGTTAACTGTTGCGAACTGAAATATCAGGACAGGATGTGAATTTACATGAGACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTg                                                                                                                                                  >  1:115660/4‑151 (MQ=255)
  aCTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGt                                                                                                                                                 >  2:404096/1‑151 (MQ=255)
       aaCTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTtat                                                                                                                                             >  1:310498/1‑150 (MQ=255)
           ggTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATaaa                                                                                                                                          <  2:538003/149‑1 (MQ=255)
           ggTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGa                                                                                                                                        <  2:262665/151‑1 (MQ=255)
               aaCTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCa                                                                                                                                     <  2:223403/150‑1 (MQ=255)
                 cTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATa                                                                                                                                   >  2:226439/1‑150 (MQ=255)
                                     aGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAAcc                                                                                                               >  2:500824/1‑150 (MQ=255)
                                      ggACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAAcc                                                                                                               >  1:629257/1‑149 (MQ=255)
                                        aaaGAATATAATTTTACATGTCACTTATTTCATTTTTTTAATGAATAAATTTTGTTTTTTTTTCATCTGGCAAAACAATTTTTAATTCTATACCACCAAGGGGGGGGTTTTTTTTAAAAAGATAAAAACCCGCCATATTCATTAACCAGtt                                                                                                           <  1:291959/149‑1 (MQ=255)
                                          aGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGtttt                                                                                                         >  1:492342/1‑151 (MQ=255)
                                                tgAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACa                                                                                                    >  1:650098/1‑150 (MQ=255)
                                                  attttACATGACTTTTAATTTATTCTTTTGATAAATTATTTTCTTTATTTTTTCATCTGGAATAAACATTTTAAATACTTTATCGCCCAGGGGGGGGTTTTGTTTAAAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACa                                                                                                    <  1:403402/146‑1 (MQ=255)
                                                      tacaggccttttAATTTATTCTTTTGTTTAATATATTTCTTTATGTTTTCATTTGGTATGAACATTCTTATTCCTTTTTCCACCAGGGGGGGGTTTTTTTTAAAAAATTCTAAACCCGCCATATTCATTAACCAGTTTTGACCAATTcc                                                                                               <  1:312694/139‑1 (MQ=255)
                                                                ttAATTCTTTCGTTGGATAAATTATTTTCTTTATTTTTTCATCTGGCATAAACATTTTAAATACTTTATCGACCAGAGGGGGGTTTTTTTTAAAAAGACCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGc                                                                                      <  1:126631/148‑1 (MQ=255)
                                                                    ttcattcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATc                                                                                >  1:259419/1‑150 (MQ=255)
                                                                        ttcTTTTGTTAAATTAATTTCTTTTTGTTTTCATCTGGCATAAACATTCTTAATCCTTTATCGCCCAGGGCGGGGGTTTTTTTAAAAAGATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaa                                                                              <  2:10422/148‑1 (MQ=255)
                                                                           gtttattgaTTTAATTTTTTTATTTTTTTTTCTGGCATGAAATTTCTTAATACTGTTTCGCCCGGGGGGGGGTCTTTTTTATAAACAACATAGCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaataa                                                                           <  1:102033/142‑1 (MQ=255)
                                                                            tttGATAAATTAATTTCTTTATGTTTTCATCTGGCATAAACATTCTTAATACTTTATCGCCCAGGGCGGGGGTTTGTTTAAAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaataat                                                                          <  2:503048/148‑1 (MQ=255)
                                                                                atgaATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatcatc                                                                   >  2:77030/1‑151 (MQ=255)
                                                                                   aattaattTCGTTATGTTTTCATCTGGCATAAACATTCTTAATCCGTTATCGCCCAGAGCGGGGTTTTTTTTAAAAAGATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAtcatca                                                                  <  1:44252/149‑1 (MQ=255)
                                                                                     ttatttTCTTTATTTTTTCATCTGGAATAAACATTTTTAATACGTTACCGACCAGGGGGGGGGTTTTTTTAAAAAGATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaa                                                               <  2:169128/150‑1 (MQ=255)
                                                                                           tCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTa                                                         >  1:42080/1‑150 (MQ=255)
                                                                                            cGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATACCGCTGAAGGCACCATCAATAATAATCATCACAACATCTaa                                                        >  1:39113/1‑150 (MQ=255)
                                                                                              ttttGTTTTCATGTGGCATAAACTTTCTTAATACGTTATCGACAAGGGGGGGGGTTTGTTTAAAAAGATCAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTaaa                                                       <  2:42800/146‑1 (MQ=255)
                                                                                                  gTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAgg                                                 >  2:12666/1‑151 (MQ=255)
                                                                                                            ggaaTGAACATTTTAAATCCTTAAGCGAGCAGAGGGGGGGTTTTTTTAAAAAGATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAttt                                           <  1:215817/144‑1 (MQ=255)
                                                                                                            ggCATGAACTTTTTTAATACTTTACCGCCAAGAGGGGGGGTTTTTTTATAAAAATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTa                                       <  2:6644/151‑1 (MQ=255)
                                                                                                            ggCATAAACATTCTTAATCCGTTATCGACCAGGGGGGGGGTTTTTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTa                                       <  2:91258/151‑1 (MQ=255)
                                                                                                                      ttCTTAATCCGTTACCGACCAGAGCGGGGGTTTTTTTATAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCt                               <  2:365769/149‑1 (MQ=255)
                                                                                                                      ttCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttg                             >  2:245054/1‑151 (MQ=255)
                                                                                                                         ttAATACGTTACCGCCCAGGGCGGGGGTTTTTTTATAAAGTTCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttg                             <  2:25468/148‑1 (MQ=255)
                                                                                                                           aatcctttACCGCCCAGGGGGGGGTTTTTTTTAAAAAGATAAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgt                            <  1:384338/141‑1 (MQ=255)
                                                                                                                           aatattttATCGACAAGGGGGGGGGTTTTTTTATAAAAATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCt                        <  2:114253/145‑1 (MQ=255)
                                                                                                                           aaTACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCt                        >  2:602868/1‑151 (MQ=255)
                                                                                                                                 ttATCGCCCAGGGGGGGGGTTTTTTTAAAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGacac                   <  2:361868/150‑1 (MQ=255)
                                                                                                                                    tCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGcc                >  2:322755/1‑150 (MQ=255)
                                                                                                                                           gggCGGGGTTTTGTTTAAAAAAATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATAc        <  2:408276/149‑1 (MQ=255)
                                                                                                                                                 gggTTTTTTTAAAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTATACAACCTTGTTGCTGACACGCCTGCAATACTTcgcg  <  2:431988/151‑1 (MQ=255)
                                                                                                                                                 gggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcgcg  >  1:523944/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCG  >  NC_000913/3412750‑3413045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑