Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,203	C→T	W36* (TGG→TGA)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,203	0	C	T	100.0%	116.6 / NA	31	W36* (TGG→TGA)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/10);  total (21/10)

TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTG  >  NC_000913/4635056‑4635344                                                                                                                                                    |                                                                                                                                              ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCAc                                                                                                                                            >  1:202345/1‑151 (MQ=255)   gagaGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACtt                                                                                                                                          >  1:616104/1‑151 (MQ=255)     gagTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTg                                                                                                                                         <  1:148149/150‑1 (MQ=255)           aaGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAAc                                                                                                                                  >  2:630019/1‑151 (MQ=255)               ggTATTGCAGCGCGATGTCCAAAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTg                                                                                                                               <  2:427848/150‑1 (MQ=255)                  aTTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCttt                                                                                                                           >  1:343033/1‑151 (MQ=255)                      cAGCGCGTTGTCCAGAATCGGACGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcgc                                                                                                                        <  2:202399/150‑1 (MQ=255)                          gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCt                                                                                                                    >  1:330935/1‑150 (MQ=255)                           cgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCt                                                                                                                    <  2:131482/149‑1 (MQ=255)                              tGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACAtt                                                                                                               >  2:595311/1‑151 (MQ=255)                                   aGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                                                           >  2:102140/1‑150 (MQ=255)                                            cgcgCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAggg                                                                                                 >  2:444148/1‑151 (MQ=255)                                             gcgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgggg                                                                                                >  1:479155/1‑151 (MQ=255)                                              cgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGc                                                                                               >  1:332637/1‑151 (MQ=255)                                                      ggCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATcc                                                                                         >  1:434171/1‑149 (MQ=255)                                                             ggCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATg                                                                                    <  1:504450/146‑1 (MQ=255)                                                                         tttCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGAtt                                                                    >  1:221904/1‑151 (MQ=255)                                                                               cAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAgg                                                              <  2:441951/151‑1 (MQ=255)                                                                                   cgacgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                                                          >  2:321502/1‑151 (MQ=255)                                                                                     acgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                                                         >  2:123424/1‑150 (MQ=255)                                                                                       gaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaat                                                      >  2:107517/1‑151 (MQ=255)                                                                                              aaTATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCg                                               >  1:341032/1‑151 (MQ=255)                                                                                                    cgcgCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGa                                         <  1:630614/151‑1 (MQ=255)                                                                                                         cAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCAt                                    >  1:333287/1‑151 (MQ=255)                                                                                                               cATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAat                               >  1:160266/1‑150 (MQ=255)                                                                                                                    ccAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTc                         <  2:23266/151‑1 (MQ=255)                                                                                                                    ccAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTc                         <  1:471600/151‑1 (MQ=255)                                                                                                                               tttAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaac              >  2:41190/1‑151 (MQ=255)                                                                                                                                   aaCATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACg          >  1:420043/1‑151 (MQ=255)                                                                                                                                            tGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTg  >  1:307288/1‑150 (MQ=255)                                                                                                                                             gTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTg  <  2:616198/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTG  >  NC_000913/4635056‑4635344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑