Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,649,2950TG46.5% 55.8 / 10.8 43intergenic (+254/‑314)ydfC/dicBuncharacterized protein, Qin prophage/Qin prophage; cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base T (7/16);  new base G (20/0);  total (27/16)
Fisher's exact test for biased strand distribution p-value = 9.43e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTG  >  NC_000913/1649156‑1649445
                                                                                                                                            |                                                                                                                                                       
tCCCTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTCCTTccc                                                                                                                                             >  2:823618/1‑151 (MQ=255)
 cccTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTtccc                                                                                                                                            >  2:277394/1‑151 (MQ=255)
 cccTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTCCTTcccc                                                                                                                                            >  1:47402/1‑151 (MQ=255)
  ccTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCg                                                                                                                                           <  1:853490/151‑1 (MQ=255)
    tGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTtcc                                                                                                                                             >  2:66528/1‑147 (MQ=255)
     gAAAAGTCAAACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGt                                                                                                                                          <  1:759673/149‑1 (MQ=255)
      aaaaTTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATACCCTGTCGTTAAATTTTCGTCTACCGTGCCCTTCCGTTTGTAGCAACCCCCGAAATGGCTCGTCGTTAATTATGGCGATGTTATTCCTTCCCCGTTg                                                                                                                                        <  2:93647/150‑1 (MQ=255)
          gTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGac                                                                                                                                   <  1:745142/151‑1 (MQ=255)
             aGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTTCCCCTTgggggaacc                                                                                                                                >  2:847839/1‑146 (MQ=255)
              gACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCg                                                                                                                               <  2:62280/151‑1 (MQ=255)
                 cATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGt                                                                                                                            <  2:18910/151‑1 (MQ=255)
                 cATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTTCCCCTTGGGGAGACCcggg                                                                                                                            >  2:146635/1‑150 (MQ=255)
                 cATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTCCCCCCTGAGGACACCcggg                                                                                                                            >  1:118842/1‑150 (MQ=255)
                 cATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGACAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTTCCCCTTGAGGGCACCcgg                                                                                                                             >  2:710058/1‑150 (MQ=255)
                            aGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGa                                                                                                                 <  1:845007/151‑1 (MQ=255)
                                cATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCCTTGAGGACACCCGGGTGTCAGGGTGAcc                                                                                                               >  2:465008/1‑149 (MQ=255)
                                  tGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCGTTGGGGGAACCCGGGTGTCAGGGTGACCAt                                                                                                             >  2:80847/1‑149 (MQ=255)
                                                  tCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTCCTTCCCCCTTGTGGGCACCCGGGTGTTAGGGTGGCCCTACGCTTTAGTGACAAccc                                                                                           >  1:775665/1‑151 (MQ=255)
                                                    atagatagCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTTCCCCCTTAGGGAACCCGGGTGTCAGGGTGACCCCACGCTTTAGTGACAAaccc                                                                                          >  1:685145/1‑150 (MQ=255)
                                                      agatagCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTTCCCCTTTAGGACACCACGGTGTTAGGGTGTCCCCACGCCTTAGTGACAAcccccc                                                                                        >  1:570098/1‑148 (MQ=255)
                                                          agCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAGCCCCGCTGCa                                                                                    <  1:41320/150‑1 (MQ=255)
                                                               gTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGccc                                                                              <  2:181947/151‑1 (MQ=255)
                                                               gTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGc                                                                                <  2:479570/149‑1 (MQ=255)
                                                                      aaTTTTCGTCGACCGGGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTa                                                                       <  1:591911/151‑1 (MQ=255)
                                                                          ttCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTTTTTCTTCCCCGTTGAGGGGAACCGGGTGTTAGGGTGACCACTAGCTTAAGGGAAAACCCCCCCGCAAAGCCCCCCGtt                                                                        >  2:425303/1‑146 (MQ=255)
                                                                                gACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTCCCCCTTGAGGGCACCCGGGTGTCAGGGTGGCCAAACGCTTAAAGGACAAACCCCCCGCCACGCCCCCTTTTTTCAAtttttt                                                             >  2:548897/1‑149 (MQ=255)
                                                                                gACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCCCTGAGGGCACCCGGGTGTTAGGGTTTCCATACACTTTAAGGAAAACCCCCCTGCCACCCCCCCTGTTTTCAAtttttt                                                             >  1:465695/1‑149 (MQ=255)
                                                                                gACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCCCTGAGGACACCCGGGTGTCAGGGTGACCCTACGCTTAAGGGACAAACCCCCTGCAACCCCCCCTGTTTTCAAtttt                                                               >  1:394434/1‑149 (MQ=255)
                                                                                gACCGCGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCCTCCCCCGTTAGGACAACAGGGGTTCAGGGTGGCCCAACGCTTCAGAGACAAACCCCCCGCAACCCCCCCTCTTTTCCAtttt                                                               >  1:491157/1‑149 (MQ=255)
                                                                                  ccGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTCCTTTCCCGTTGGGGACACCCGGGTGTTAGGTTGACCATACGCTTAAATGACAAACCCCCCGCAAACCCCCCTGTTATCAAtttttt                                                             >  2:649204/1‑147 (MQ=255)
                                                                                               gTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTTTTTCTTTCCCCTTGGGGGAACCCGGGTGTCAGGGTGACCCTACGCTTTAGTGACAAACCCCCTGCAACGCCCCCTGTTTTCAATTTTTTTGGGACGctttg                                                 >  2:827761/1‑146 (MQ=255)
                                                                                                       aaCCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCCCTGAGGGAACCCGGGGGTTAGGTTGACCCAACGCTTTAGTGACAACCCCCCTCCCACGCCCCCTGTTTTTCATTTTTTGGGGAAGTTTTGGGGGATCAggt                                      >  1:699883/1‑149 (MQ=255)
                                                                                                         cccGCGAAATGGCGCGGCGGTAAGTATGGCGGGGGTATTTCTTCCCCGTTGAGGACACCCGGGTGTCAGGGTGAACATACGCTTAAAAGACAAACCCCCCGCAACGCCCTCTGTTATCAATTTTTTTGTGACGGTTGGCGGGATCAGtttt                                    >  2:790391/1‑151 (MQ=255)
                                                                                                                   ggCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGa                           <  1:149403/150‑1 (MQ=255)
                                                                                                                      gcggcgGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGc                       <  2:497121/151‑1 (MQ=255)
                                                                                                                            gTAAGTATGGCGGGGGTATTTCTTCCCCGTTGAGGACACCCGGGTGTCAGGGTGGCCCTACGCTTAAGTGACAACCCCCCTGCAACGCCCCCTGTTTTCAATTTTTTGGTGGCGGTTGGCGGGATCAGTTTTACTCCGTGACTGctc                     >  1:338336/1‑147 (MQ=255)
                                                                                                                              aaGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTgccgc               <  1:66525/151‑1 (MQ=255)
                                                                                                                                  aTGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCt            <  1:146622/150‑1 (MQ=255)
                                                                                                                                    ggCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCtttt         <  2:355354/151‑1 (MQ=255)
                                                                                                                                    ggCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCtttt         >  1:206362/1‑151 (MQ=255)
                                                                                                                                      cGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCttttt        >  2:687153/1‑150 (MQ=255)
                                                                                                                                        ggggTTATTCCTTCCCCGTTGAGGACACCGGGATGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCtttt         >  1:495625/1‑148 (MQ=255)
                                                                                                                                          ggTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTaa      >  2:643938/1‑149 (MQ=255)
                                                                                                                                            ttATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTg  >  1:176895/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                       
TCCCTGAAAAGTCAGACCATAACGCGAAAGCGCATGGCGAGGTAGCTGGTTCATAGATAGCCTGTCGTTAAATTTTCGTCGACCGTGCGCTTCCGGTTGTGGCAACCCGCGAAATGGCGCGGCGGTAAGTATGGCGGGGTTATTCCTTCCCCGTTGAGGACACCGGGTTGTCAGGTTGACCATACGCTTAAGTGACAACCCCGCTGCAACGCCCTCTGTTATCAATTTTCTGGTGACGTTTGGCGGTATCAGTTTTACTCCGTGACTGCTCTGCCGCCCTTTTTAAAGTG  >  NC_000913/1649156‑1649445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: