Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,639	0	A	C	45.5%	58.1 / 11.1	44	T64P (ACC→CCC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base A (17/7);  new base C (0/20);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 6.75e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATG  >  NC_000913/2265490‑2265785                                                                                                                                                        |                                                                                                                                                    aTTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGacac                                                                                                                                                    <  1:568519/151‑1 (MQ=255) aTTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGacac                                                                                                                                                    >  1:507203/1‑151 (MQ=255)      aaCGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTg                                                                                                                                                >  2:715685/1‑150 (MQ=255)      aaCGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGa                                                                                                                                               <  2:525220/151‑1 (MQ=255)         ggCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGc                                                                                                                                            <  1:538314/151‑1 (MQ=255)                 gctgctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GAccc                                                                                                                                     <  1:5442/149‑1 (MQ=255)                      tgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGtt                                                                                                                               >  2:581976/1‑150 (MQ=255)                            aGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTg                                                                                                                         >  1:694960/1‑150 (MQ=255)                              gatattgatattCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACt                                                                                                                      >  1:220463/1‑151 (MQ=255)                                   tgatatcCGGCCGCCCCCTCCCCGCGGCGCCGCCCCGCTGTAAAAATTGTTTTTTTCTGTTGTCCGTACGGGGCTAAAAGTAAAAGGGCGTTAAAAA‑GG‑TGATGATACTTTTGACCCCGTGACGCT‑GCCCCGCCGTTACGTTGACTtctc                                                                                                                  <  2:490446/150‑1 (MQ=255)                                      tatcCAGCCGCCCACCCCCCGGGGCCCCGCTCCGCTGAAAAAAATGTTTTTTTCTGATGCCCGCCCGGGGCAAAAAGAAAAAGGGC‑TTTAAAA‑GGTTGATTATTTCGTGGACCCCGTCACGCT‑GCCCCGCCGTTACGTTGACTTCTCCt                                                                                                                <  1:87967/149‑1 (MQ=255)                                         tCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTc                                                                                                           >  2:393830/1‑151 (MQ=255)                                          cagcccccccccccccGGGGTGCCGCTACGCTGTAAAAAATGCTTTTTTTTGATGCCCGTCCGGGGCTAAAAGTAGAAGAGCTTTAAAAG‑GG‑TTATGATTTGTTTGCCCCGGTGACGTT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCg                                                                                                          <  2:503028/138‑1 (MQ=255)                                           aGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAATGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCg                                                                                                          >  1:211425/1‑150 (MQ=255)                                                 ccgcTGCCCGCGGTGCCGCTTGGCGGAAAAAATTGTTTTTTTTTCAGGTCCGGCCGGGGCAAAGGGTAAAAAAGGTTTTGAAG‑GT‑TGACGATAGCGTTGACA‑GATTTCGCTAGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGc                                                                                                     <  1:21470/146‑1 (MQ=255)                                                   aCTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACg                                                                                                 >  1:207105/1‑151 (MQ=255)                                                       cccGGGGGGCCGCTCCGTTGTAAAAAATGTTTTTTTCTGTTGTCCGCCCGGGGCTGAAAGTAAAAGAGCTTTAAAAG‑GG‑TGATTATATTTTTGACCCCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAAc                                                                                                  <  2:890319/146‑1 (MQ=255)                                                       cccGGGGGGCCGCTACGCTGAAAAAAATGTGTTTTTCTGATGCCCGTCCGGGGCTAAAAGTAGAAGAGCGTTTAAAG‑GG‑TGATGATTTCGTTGCCCCGGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACg                                                                                                 <  2:490466/147‑1 (MQ=255)                                                          gcgGTGCCGCTCCGCTGAAAAAAATGTTTTTTTCTGATGCCCGTACCGGGCTAAAAGAAGAAGAGCGTTAAAAA‑GG‑GGATGATTTCTTTGACCCCGTGCCGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAatat                                                                                            <  2:569428/149‑1 (MQ=255)                                                           gggTCCCTCTACGTTGAAAAAAATGTTTTTTTCTGATCCCCGTACGGGGCTGAAAGAAAAAGGGTTTTAAAAA‑GT‑TGATGATATCGTTGACCCCGGGACGCT‑ACCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTc                                                                                         <  1:668308/150‑1 (MQ=255)                                                           cgGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTc                                                                                         >  1:79799/1‑151 (MQ=255)                                                                ccgccccGCTGTAAAAAGTGCTTTTTTCTGTTGTCCGTCCGGGGATAAAAGAAGAAGGGTTTTAAAAA‑GG‑TGATGATTTCTTTGACCCCGGGCCGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCtt                                                                                       <  2:573778/142‑1 (MQ=255)                                                                 cGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATg                                                                                   >  2:417759/1‑151 (MQ=255)                                                                  gCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATgg                                                                                  >  2:223219/1‑151 (MQ=255)                                                                   cTACGTTGAAAAAAGTGGTTTTTTCTGATGCCCGTACGGGGGAAAAGGAAGAAGGGCTTTACAAG‑GG‑TGATGATATGGTTGACACGGGGCCGCG‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATgg                                                                                  <  2:439572/150‑1 (MQ=255)                                                                            aaaaattgtttttttCTGATGTCCGTGCGGGGCAAAAAGAAGAGGAGCTTTTAAAA‑GG‑TGATAATATCGTTGACACCGGGACGCT‑AACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAagaaga                                                                        <  1:581920/141‑1 (MQ=255)                                                                            aaaaaaggtttttttCTGTTGCCCTTACCGGGCTGAAGGTAGAGGAGCTTTAAAAG‑GG‑TGATTATTTCGTTGCCCCCGTGACGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaagaag                                                                         <  2:768252/140‑1 (MQ=255)                                                                            aaaaaaTTCTTTTTTTTGATGTCCGTCCGGGGCAGAAAGAAAAAGAGCTTTTAAAG‑GG‑TGATGATATCTTTGACCCCGTGCCGCT‑ACCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaagaa                                                                          <  1:122055/147‑1 (MQ=255)                                                                            aaaaaaTGCTTTTTTCTTATGCCCGTACCGGGTTAAAAGAAGAAGAGTTTTTAAAA‑GG‑TGATGATATCGTTGACCCCGTGCCGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaaga                                                                           <  2:31367/146‑1 (MQ=255)                                                                              aaagtggtttttttGGATGCCCGTCCGGGGGGAAAAGGAAAAGGGCTTTAAAAG‑GG‑GGATGATATGGTTGACCCCGTGACGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaaga                                                                           <  2:552968/138‑1 (MQ=255)                                                                                     ttttttCTGATGCCCGTCCGGGGCTAAAAGTAAAAGAGCGTTTAAAA‑GG‑TGTTGATTTCGTTGACCCCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATAccc                                                                <  2:666376/149‑1 (MQ=255)                                                                                      ttttgttggtGTCCGTCCGGGGCTAAAAGAAG‑AGGGGGTTTAAAAGGG‑TGATTTTATCGTTGCCCCGGGGCCGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATAcccc                                                               <  1:375873/141‑1 (MQ=255)                                                                                            tgattcccGTACGGGGTTGAAAGAAGAAGGGTTTTTAAAG‑GG‑GAATGATTTTGTTGCCCCCGTCGCGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATAc                                                         <  2:424452/144‑1 (MQ=255)                                                                                                    gtccggggCTGAAAGTAGAAGAGCGTTAAAAA‑GT‑TGAGGATATCGTTGACACCGTGCCGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCaa                                                <  1:776784/146‑1 (MQ=255)                                                                                                     tACGGGGTTGAAAGAAAAAGAGCTTTTAAAA‑GG‑TGATGATATCGTTGACCCCGTGCCGCG‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCa                                                 <  2:458687/149‑1 (MQ=255)                                                                                                           gagaaaaGAAAAAGAGCGTTAAAAA‑GG‑TGATTATCTCGTTGACCCGGGGCCGCT‑ACCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAg                                         <  1:299319/147‑1 (MQ=255)                                                                                                           gCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATc                                           >  1:843135/1‑149 (MQ=255)                                                                                                              gAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGAtt                                      >  1:222158/1‑151 (MQ=255)                                                                                                                       aggattttttAAA‑GGTTGATATTATCTTGGACCCCGGGACCCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTgaa                                   <  1:210334/140‑1 (MQ=255)                                                                                                                        gggcttttAAAG‑GG‑TGATGATTTCGTTGCCCCCGGGACGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGt                            <  2:507246/146‑1 (MQ=255)                                                                                                                        gagCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGt                            >  2:500593/1‑151 (MQ=255)                                                                                                                                cAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGttt                    >  2:844155/1‑151 (MQ=255)                                                                                                                                   a‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATg                 >  2:639733/1‑151 (MQ=255)                                                                                                                                     gg‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGc                >  1:772439/1‑151 (MQ=255)                                                                                                                                        tgataaTATCGTGGCCCCGTTGACGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTAAGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGc                <  2:112695/149‑1 (MQ=255)                                                                                                                                              tgtcttcaacacCGTGGCGCT‑GCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGcc               <  2:207124/136‑1 (MQ=255)                                                                                                                                                    tGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATg  >  1:25339/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                    ATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAA‑GG‑TGATGATATCGTTGACACCGTGACGCT‑GACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATG  >  NC_000913/2265490‑2265785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑