Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913328,8070AT45.6% 52.4 / 29.7 46D176E (GAT→GAA) betIcholine‑inducible betIBA‑betT divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (6/19);  new base T (21/0);  total (27/19)
Fisher's exact test for biased strand distribution p-value = 5.07e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGGTGCGACCGCTGGTGGCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGGCTGCTGACCTGC  >  NC_000913/328661‑328957
                                                                                                                                                  |                                                                                                                                                      
cGAAGGTGCGACCGCTGGTGGCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATcc                                                                                                                                                     <  1:889287/150‑1 (MQ=255)
             gCTGGTGGCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCt                                                                                                                                       <  1:20515/151‑1 (MQ=255)
              cTGGTGGCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGGATTAACGCGGGGTTTTTTCAGCGGGTTGTCGccc                                                                                                                                      >  2:773072/1‑149 (MQ=255)
                ggtggCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGAATAGCGCGGGGTTTTTTCACCGGgtt                                                                                                                                             >  2:738754/1‑142 (MQ=255)
                        aGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCcgcgc                                                                                                                            <  2:781100/151‑1 (MQ=255)
                         ggTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCcgcgcg                                                                                                                           <  1:652696/151‑1 (MQ=255)
                         ggTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCCGGGGATTAACGCGGGGTTTTTCCCCCGGGTTGCCGccc                                                                                                                                      >  2:458524/1‑138 (MQ=255)
                            ataACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCGCGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAg                                                                                                                        <  1:678394/151‑1 (MQ=255)
                             taACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGAGTAGCGCGGGGGTTTTTCAGCGGGTTGGCGCTCCGAGCCGCGcgccgc                                                                                                                       >  2:485669/1‑151 (MQ=255)
                                ccaccaTGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCAt                                                                                                                    <  1:392202/151‑1 (MQ=255)
                                ccaccaTGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCCGGGGAATAGCGGGGGGTTTTTTCAACGGGTTGCCCCTCCGAGCCCCGCgccgcc                                                                                                                      >  2:805849/1‑149 (MQ=255)
                                       gTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCat                                                                                                             <  2:121977/151‑1 (MQ=255)
                                         atataAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCatca                                                                                                           <  1:360066/151‑1 (MQ=255)
                                          tataAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGGATTAGCGCGGGGTTTTTTCACCGGGTTGCCGCCCCGAGCCGCGCGCCGCCAAAATACa                                                                                                              >  2:894779/1‑147 (MQ=255)
                                              aaGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGGAATAACGCGGGGTTTTTTCAGCGGGTTGCCGCCCCCAGCCGCGCGCCGCCCTAAACCaacca                                                                                                          >  2:746923/1‑143 (MQ=255)
                                                   gctgTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGAATAGCGCGGGGGTTTTTCCGCGGGTTTTCGCCCAGAGCCGCGCGCAGCCCTAATCCatccaataaccc                                                                                                   >  2:523775/1‑140 (MQ=255)
                                                    ctgTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGGATTAGCGCGGGGGTTTTTCAGCGGGTTGTCGCCCACAGCCGCGCGCCGGCACAATCCATCAATCAGCGCgg                                                                                                 >  2:317035/1‑150 (MQ=255)
                                                      gTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGcc                                                                                               <  1:433581/150‑1 (MQ=255)
                                                      gTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCa                                                                                              <  2:655900/151‑1 (MQ=255)
                                                        tCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCCGGGGAATTGCGGGGGGTTTTTCCCACGGGTTGCCCCCCCCAGCCGCGCGCCGCCATAATACATCAAACAACGCgg                                                                                                 >  2:539194/1‑146 (MQ=255)
                                                             cATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGAATAACGCGGGGTTTTTTCAGCGGGTTGGCGCCCCCAGCCGCGCGCAGCCATAAACCATCAAACAACGcggcccggcc                                                                                          >  2:464457/1‑148 (MQ=255)
                                                                     aCATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCCGGGGATTAGAGCGGGGTTTTTTCAACGGGTTGCCGCCCCGAGCCGCGCGCCGCCCTAAATCATCAATCAACGcggcccggcccgaaccc                                                                                   >  2:615832/1‑140 (MQ=255)
                                                                         cACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCt                                                                            <  2:413692/150‑1 (MQ=255)
                                                                            gTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGc                                                                        <  2:901408/151‑1 (MQ=255)
                                                                                cctcGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTg                                                                     <  1:880018/150‑1 (MQ=255)
                                                                                 ctcGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGATTAGCGCGGGGTTTTTTCAGCGGGTTTGCGCCCCGAGCCGCGCGCAGCCATAAACCATCAATCAACGCGGCCCGGCCGCAGCCCGCCTCCCCTGCCTGtt                                                                   >  2:120266/1‑151 (MQ=255)
                                                                                    ggTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGATTAGCGCGGGGTTTTTTCAGCGGGTTGTCGCTCAGAGCCGCGCGCCGCCAAAATCCCTCAATCAACGCGGCCAGGGCCGAGCCCGCCTCCTCTGCCTGtt                                                                   >  2:275045/1‑148 (MQ=255)
                                                                                     gTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTcgcg                                                               <  2:813517/151‑1 (MQ=255)
                                                                                      ttAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGAAGGGAAATAGCGCGGGGTTTTTTCACCGGGTTTTCGCTCCCAGCCCCGCGCCGCCCCAATCCCTAAATCACCGCGGCCACGCCCTAACCCCCCTCCCGTGccccgt                                                                   >  1:504168/1‑142 (MQ=255)
                                                                                        aaTCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGAATAGAGCGGGGTTTTTTCACCGGGTTGGCGCCCACAGCCGCGCGCCGCCCTAATCCATCAAACAACGCGGCCCGGCCGCAGCCCCCCTCCTGTGCCTGttttcgc                                                              >  2:910750/1‑144 (MQ=255)
                                                                                         aTCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGGCCGGGGATTAACGCGGGGTTTTTTCAGCGGGTTTTCGCCCCGAGCCGCGCGCCGCCCAAATCCATCAATCAGCGCGGCCAGGCCGCAGCCCCCTTCCTCTGCCCGTTTGCGCgg                                                            >  2:48679/1‑150 (MQ=255)
                                                                                              tGGGTAGATGCTGAGTGATAAAGTGGCGGGTCCGGGGATTAACGCGGGGTTTTTTCAACGGGTTTTCGCCCCGAGCCGCGCGCCGCCCCAATCCCTCAATCAACGCGGCCCCGCCGCAACCCCCCTCCCGTGCCCGTTTTCGCGGCCAtt                                                       >  1:450135/1‑150 (MQ=255)
                                                                                               gggTAGATGCTGAGTGATAAAGTGGCGGGGCAGGGGATTAGCGCGGGGTTTTTTCCGCGGGTTGTCGCCCCGAGCCCCGCGCCGCCATAATCCATCAATCAACGCCGCCAGGCCGTAGCCCGCCTCCTCTGCCCGTTTGCGCGGCaaat                                                       >  1:746449/1‑147 (MQ=255)
                                                                                                         tGAGTGATAAAGTGGCGGGTCAGGGGAATAGCGCGGGGTTTTTTCAACGGTTTTCCGCCCCGAGCCGCGCGCAGCCATAATCCATCAAACAACGCGGCCCGGCCGGAGCCCCCCTCCTGTGCCCGTTTGCGCGGCAATTCGCCACGgaa                                             >  2:179079/1‑149 (MQ=255)
                                                                                                         tGAGTGATAAAGTGGCGGGTCAGGGGAATAAAGCGGGGTTTTTTCAGCGGTTTGTCGCCCCGACGCGCGCGGCGGCATAAACCCCTAATCAACGCGGGCCGGCCGCAGGCCCCTCTCCGTGCCTGTTCGCGCGGCCAtt                                                       >  1:586362/1‑139 (MQ=255)
                                                                                                               aTAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTc                                      <  1:824284/150‑1 (MQ=255)
                                                                                                                 aaaGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCAc                                    <  1:408446/150‑1 (MQ=255)
                                                                                                                            tCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACa                         <  1:623003/150‑1 (MQ=255)
                                                                                                                              aGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGc                       >  1:732577/1‑150 (MQ=255)
                                                                                                                                 gAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAgc                   >  1:102894/1‑151 (MQ=255)
                                                                                                                                  aaTTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAgcg                  >  2:904094/1‑151 (MQ=255)
                                                                                                                                      aGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGcggcgg              >  2:276243/1‑151 (MQ=255)
                                                                                                                                          cgGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGgctg           <  2:114572/150‑1 (MQ=255)
                                                                                                                                           gggTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGgctgct         <  1:396960/151‑1 (MQ=255)
                                                                                                                                               tttATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGGCTGCTGAcc     >  2:533711/1‑151 (MQ=255)
                                                                                                                                                ttATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGGCTGCTGACct    >  2:584424/1‑151 (MQ=255)
                                                                                                                                                  aTCCAGCGGTTTGCCGCTTAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGGCTGCTGACctgc  >  2:101723/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CGAAGGTGCGACCGCTGGTGGCGGAGGTATAACCACCATGTATATAAAGCTGCTGTTCTGCCATTCGGGACATCACGTCTCCTCGGTTAATCGGTGGGTAGATGCTGAGTGATAAAGTGGCGGGTCAGGGAATTAGCGCGGGTTTTATCCAGCGGTTTGCCGCTCAGAGCCGCGCGCAGCCATAATCCATCAATCAGCGCGGCCAGGCCGTAGCCCGCTTCCTGTGCCTGTTCGCGCGGCAATTCGCGACGAAACTCGCTCACCAGATTCGACAGCAAGCGGCGGCTGCTGACCTGC  >  NC_000913/328661‑328957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑