Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 884,346 | 0 | C | G | 47.3% | 87.0 / 17.4 | 56 | A225G (GCG→GGG) | mdfA | multidrug efflux system protein |
Reads supporting (aligned to +/- strand): ref base C (2/27); new base G (26/0); total (28/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.14e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTT > NC_000913/884197‑884490 | tttgtttgCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggc < 1:734224/150‑1 (MQ=255) ttgtttgCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcg < 1:746199/150‑1 (MQ=255) ttgtttgCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcg < 1:242561/150‑1 (MQ=255) aTTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGCGCTggg > 2:661622/1‑150 (MQ=255) gCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCg < 2:598132/151‑1 (MQ=255) gATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCGGGGGCCgggggttgt > 1:836569/1‑141 (MQ=255) aTCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGGGCTGGGGTTCGtt > 2:845765/1‑147 (MQ=255) ccTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGt < 2:888206/146‑1 (MQ=255) ccTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGt < 2:45757/146‑1 (MQ=255) tttCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGtt < 2:604995/150‑1 (MQ=255) tCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTCGTTAGTCTGCCGGTGCTgggg > 2:786056/1‑149 (MQ=255) tCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGc < 1:747424/150‑1 (MQ=255) cGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCGGGCGGTGGGGTTTGTTAGTCTGCCGTTGCTgggggggggcg > 2:82234/1‑142 (MQ=255) gCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGCGGCGGCGGGGGTCGTTTGTTTGCCGTTTCTGGCGGGGGGCGcccccg > 1:724078/1‑146 (MQ=255) gCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTc < 2:1088/150‑1 (MQ=255) gCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCg < 1:166862/151‑1 (MQ=255) cTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGGGCTGGGGTTCGTTAGTCTGCCGTTGCTGGGGGGGGGCGCCCCgccgcc > 2:393699/1‑151 (MQ=255) tGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGGTGGGGGTCGTTTGTCTGCCGGTGCTGGCGGGGGTCGCCCAGGCGcc > 2:888360/1‑150 (MQ=255) gAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCg < 1:402664/150‑1 (MQ=255) aaaCCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGTGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGAt < 2:139300/151‑1 (MQ=255) aaaCCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCTGGGGGTTGGTTGTCTGCCGTTGCTGGCggggggtggcccgccg > 2:757760/1‑134 (MQ=255) cACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGCGCTGGGGGTCGTTAGTGTGGCGTTGCTGGCGGGGGGCGccccggcggcgggt > 1:780289/1‑134 (MQ=255) cACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGGTGGGGTTTGGTTGTCTGCCGGTGCTGGGGGGGGTCCGCCCCTCGCCGGCTatcatc > 1:568087/1‑151 (MQ=255) cgcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCTGGGGTTTGTTTGTCTTCCGTTTCTTGCGGGGGGCGCCCCCTCGcccctttt > 2:642657/1‑139 (MQ=255) gcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCTGGGGGTTGTTTGTCTGGCGGTGCTGGGGGGGGGCGCCCCGTCGGCggctattattctt > 2:798128/1‑139 (MQ=255) gcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCAtt < 1:841280/151‑1 (MQ=255) tataGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCGGGGGGTCGTTTGTCTGCCGTTGGTGGCGTGGGTCGCCCCGTTGCCGCTTTTTATCCtt > 2:380123/1‑148 (MQ=255) ggCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGGGGTGGGGGTTGTTTGTTTGCCGTTGGTGGGGGGGGTCGGCCCCTCGCCGGTTTTTATCCTTaacag > 2:511081/1‑145 (MQ=255) ggCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCg < 1:845669/151‑1 (MQ=255) gCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGa < 2:142643/151‑1 (MQ=255) gaAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGATGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCa < 1:499098/150‑1 (MQ=255) aaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGGGCTGGGGTTCGTTTGGGTGCCGTTGGTGGGGGGGGGGGCCCCGTCGCCGCTTATTATCATTATCGGCgggggg > 1:141036/1‑144 (MQ=255) aaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGtt < 2:860341/151‑1 (MQ=255) tGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCCGGGGTTCGTTTGTCTGCCGTTGCTGGCGGGGGTCGCCCCGTCGCCCATTATCATCCTTACCGGccggccggt > 2:665329/1‑140 (MQ=255) gTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGa < 1:7254/149‑1 (MQ=255) cACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGTGGGGCTGGGGTTCGTTTGTCTGCCGGTGGTGGGGGGGGTTGCCCCGGCGGCGGTTATCATTATTATCGGCGGGGAGGTGag > 2:623563/1‑148 (MQ=255) cACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGagcag < 2:161865/151‑1 (MQ=255) tGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTCGTTTGTCTGCCGGTGGTGGCGGGGGGCGCCCCGTCGCCGGTTATTATCATTACCGGCggggcggt > 2:118269/1‑135 (MQ=255) gAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCTGGGGGTCGTTAGTCTGCCGTTGCTGGCGGGGGGCGCCCCGGCGCCGGTTATCATCATTATCGGCGGGCAGGTGgggagcgct > 1:907728/1‑148 (MQ=255) gAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTa < 2:86221/150‑1 (MQ=255) gAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTAt < 2:471555/151‑1 (MQ=255) aaGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCTGGGGGTCGTTTGTCTTCCGGTGCTGGGGGGGGTCGCCCCGTCGCCGGTTATCATCATTAACCGCGGGCAGGTGGGCAGCTAt > 2:845056/1‑149 (MQ=255) aGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGaa < 1:831194/151‑1 (MQ=255) aaCTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCTGGGGGTCGTTTGTCTGCCGTTGTTGGGGGGGGGCGGCCCGGCGCCCCGTATCATCATTTACGGCGGGCCGGTGag > 1:477450/1‑139 (MQ=255) cTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAatat < 2:248180/150‑1 (MQ=255) tCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCCGGGGGTCGTTAGTCTGCCGTTGCGGGGGGGGGTCGCCCCCGCCCCCGTTTTCCTCATTACCCGCGGGCAGGTGAGCAGCCCTGaa > 1:395869/1‑146 (MQ=255) cGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCTGGGGTTTGTTTGTCTGCCCTTGCTGGGGGGGGTCGCCCCGTCGCCGCTTATTATCATTATCCGCGGGCAGTTTAGCAGCTATGAatat > 2:432031/1‑148 (MQ=255) cGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGCGCTGGGGTTTGTTAGTCTGCCGGTGCTGGCGTGGGGCGCCCCGTCGCCGATTATCATCCTTTCCCGCGGGGAGGTGAGCAGCTATGAatattg > 2:76883/1‑148 (MQ=255) cGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATg < 1:458430/149‑1 (MQ=255) ataAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAg < 1:123170/150‑1 (MQ=255) taAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCTGGGGGTCGTTAGTCTGCCGGTGCTGGGGGGGGGCGCCCCCCCGCCCGTTATCATCATTATCGGCGGGCAGGTGTGCAGCGCTGAAAATGGCGTTCTGCAAGTg > 1:564722/1‑151 (MQ=255) aaGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGCGGCGCTGGGGGTTGTTTGTTTGCCCCTGCTTGGGGGGGTCGCCCCGCCGCCGGTTATTATCATTTACCGCGGGGAGGTGAGGAGCCAAGAatattggtttcttccaag > 1:766283/1‑135 (MQ=255) aaGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGc < 2:842222/151‑1 (MQ=255) cGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTa < 1:18839/151‑1 (MQ=255) gCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAAt < 1:393663/151‑1 (MQ=255) gtgGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGt < 2:862779/150‑1 (MQ=255) gCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACt < 1:531492/151‑1 (MQ=255) gCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACt < 2:652804/151‑1 (MQ=255) cGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACtt > 1:814645/1‑151 (MQ=255) | TTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTT > NC_000913/884197‑884490 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |