Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	46.7%	77.3 / 23.9	60	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (5/27);  new base G (28/0);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 2.43e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730                                                                                                                                                  |                                                                                                                                                        cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCg                                                                                                                                                     <  2:74654/150‑1 (MQ=31) cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa                                                                                                                                                    <  1:410491/151‑1 (MQ=31)    tGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGATTTGAAcc                                                                                                                                                 >  1:104748/1‑151 (MQ=14)         tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCg                                                                                                                                             <  2:191376/150‑1 (MQ=37)           aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAccccc                                                                                                                                              >  2:14814/1‑147 (MQ=25)             ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGACCCCCGATa                                                                                                                                          >  1:270252/1‑149 (MQ=255)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCCTACGtt                                                                                                                                      >  2:786199/1‑148 (MQ=255)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGtt                                                                                                                                      >  2:791942/1‑148 (MQ=255)                     cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGta                                                                                                                                <  1:98593/151‑1 (MQ=255)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCATACGGTGCCgccta                                                                                                                              >  2:185011/1‑145 (MQ=14)                           gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca                                                                                                                          <  2:435677/151‑1 (MQ=255)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑Ctt                                                                                                                      <  1:180416/151‑1 (MQ=255)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑Ctt                                                                                                                      <  2:553269/151‑1 (MQ=255)                                cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑Cttt                                                                                                                     <  2:104756/150‑1 (MQ=255)                                cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTc                                                                                                                    <  2:738637/151‑1 (MQ=255)                                cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTCGAACCCCCCGTACATTGCCGTAAacacaactt                                                                                                                      >  2:596329/1‑146 (MQ=14)                                      ttGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTTGCAACCCCCAAACGTTGCCGGATTCACA‑CCTTTCacg                                                                                                                >  1:887296/1‑147 (MQ=11)                                       tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCCTACCGTTCCGGATACACACCTTTccgg                                                                                                                 >  1:799919/1‑146 (MQ=11)                                           gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCGATACGTTGGCGTATAAAAA‑ATTTTCAGGGGTg                                                                                                            >  1:271813/1‑148 (MQ=14)                                           gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCCTACGTTTCCGTATACACA‑CTTTCCAGGCg                                                                                                              >  1:286297/1‑146 (MQ=255)                                              ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTTGGAAACCCGATTAGGTGGCGTATTTACA‑Cttttcctgcgttgtgctt                                                                                                      >  1:363138/1‑136 (MQ=1)                                              ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCCCAAAGGTGCCGGATACAAA‑CATTTCAGGCGTg                                                                                                            >  1:212118/1‑145 (MQ=11)                                              ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCtt                                                                                                      <  2:454294/151‑1 (MQ=255)                                                ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACCTTGCCGGATACACA‑CTTTCCAGGCGTGCTcc                                                                                                        >  2:213279/1‑147 (MQ=255)                                                 cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGGACCCCCCATACGTTGCCGTATACACC‑CTTTCCAAGCGTGCTCCtt                                                                                                      >  2:47349/1‑148 (MQ=17)                                                  ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGGTTCCGTATAAACA‑CTTTCCAGGCGGGCTCCTTCAGc                                                                                                  >  1:776490/1‑151 (MQ=18)                                                        ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCg                                                                                            <  1:780006/151‑1 (MQ=255)                                                         gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCgg                                                                                           <  2:496261/151‑1 (MQ=255)                                                                    ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCGCAAAGTTTCCGTAAAAAAC‑CTTTCCCGGGGGGCCCCTTCAGCCCCCCGGGCAccccccc                                                                                 >  2:855886/1‑145 (MQ=14)                                                                          tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCGATACGTTGCCGGATACACA‑CTTTCCCGGCGGGCTCCTTCAGCCACTCGGACACC‑CCACCCAAtttt                                                                         >  2:893646/1‑149 (MQ=14)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGt                                                                      <  1:882274/151‑1 (MQ=255)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGt                                                                      <  2:464969/151‑1 (MQ=255)                                                                                    ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTc                                                               <  1:230791/151‑1 (MQ=255)                                                                                      aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCt                                                              <  1:687085/150‑1 (MQ=255)                                                                                       cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCCAATTGTCGTTCCTGTCtt                                                             >  2:100291/1‑150 (MQ=255)                                                                                       cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTTGAACCCCCCATACGTTGCCGTATACAAA‑CTTTCCAGGGGGGGTCCTTCAGCCCCCCGGACACC‑CCACCAAAATGTCGTTTCTGTCtt                                                             >  1:683654/1‑150 (MQ=14)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTTGGAACCCCCATACGTTTCCCTATACACA‑CTTTCCAGGGGTGGTCCCTCAGCCACTCGGGCACC‑CCACCAAAATTTCGTTTCTGTCtt                                                             >  1:94469/1‑148 (MQ=14)                                                                                           aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTgg                                                        <  2:564006/151‑1 (MQ=255)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGACCCCCCATACGTTGCCGTATAAACACCTTTCCA‑GCGGGGTCCCTCAGCCACCCCGACACC‑CCCCCAAAATTTTGTTCCTTTCtt                                                             >  2:366472/1‑144 (MQ=2)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTTGCGGATATACA‑CCTTTCACGCGGGGCCCTTCAGCCACTCCGACACC‑TCCCCCAAATGTCGTTTCTGTCTTGCTGGa                                                       >  2:740729/1‑149 (MQ=14)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTTGGACCCCCCATACGTTGCCGTATACACA‑CTTTTCAGGCGGGCTCCTTCCTCCACCCGGACACC‑CCACCAAAATTTCGTTCCTGTCtttttgggaaggg                                                  >  1:698704/1‑139 (MQ=14)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGACCCCCCATACGTTTTCGTATAAACA‑CATTCCCGGGGTGCTCCTTCTGCCACTCCGACACCACCACCAAA‑TGTCGTTTCTGTTTTGCTGGAACAGgcg                                                >  1:26754/1‑150 (MQ=9)                                                                                                     gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCCAACCCCCCATACGTTTCCGTATACACA‑CTTTTCCGGCGGGCTCCTTCTTCCACTCGGACACC‑TCACCAAATTGTCGTTTCTGTCTTGCTGGGACGGgcgc                                               >  1:887597/1‑150 (MQ=14)                                                                                                        cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGATTTGGACCCCCCCTACGGTGCCGTATACACA‑CCTTTCAGGCGTGCTCCTTCAGCCACCCGGACACC‑CCCCCCAAATTTCGTTCCTTTCTTTCTGGAACGGGCGCCaa                                            >  1:826582/1‑150 (MQ=14)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGACCCCCCATACGTTTTCGTATACACA‑CCTTTCAGGCGTGCTCCTTCCGCCCCTCGGGCACC‑CCCCCAAAATGTCGTTTCTGTCTTGCTGGAACGGGCGCTAAttt                                         >  1:670064/1‑151 (MQ=17)                                                                                                           tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAttt                                         <  1:274531/150‑1 (MQ=255)                                                                                                             gTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATCGAAACCCCCCCACGTTTCCCTATACACA‑CCTTCCAGGGGGGGTCCTTCAGCCCCCCCGACACC‑CCACCCAAATTTTGTTCCTGTCTTGGTGGAACCGGCGGTAAttt                                         >  1:544863/1‑148 (MQ=9)                                                                                                             gTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAgg                                      <  1:277807/151‑1 (MQ=255)                                                                                                                cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                   <  1:175785/151‑1 (MQ=255)                                                                                                                cGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGCTTGAACCCCCGCTACCGTTTCGTATACACA‑CTTTCCACGCGGGCTCCTTTAGGCACTCGGACACC‑CCACCCAATTTTCGTTCCTTTCTTTCTTGGACggggcccaactttggg                                     >  1:382065/1‑136 (MQ=2)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGATACGTTGCCGGAAACACA‑CTTTCCAGGCGGGCTCCTTCAGCCACTCGGGCACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTTgggga                                   >  2:136069/1‑148 (MQ=17)                                                                                                                         cttTAAATATGGCGGTGAGGGGGGGGATCGAAACCCCGATACGTTGGCGTATACACA‑CTTTTCCGGCGTGCTCCTTCCGCCACCCGGGCACC‑TCACCCAATTTTTGTTCCTGTCTTGCTGGAACGGGCGCCAATTTATGGGAAACATGAcc                          >  2:270829/1‑151 (MQ=17)                                                                                                                           ttAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCt                         <  2:30327/150‑1 (MQ=255)                                                                                                                             aaaTATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGa                       <  2:776574/150‑1 (MQ=255)                                                                                                                                   ggCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                <  1:14814/151‑1 (MQ=255)                                                                                                                                     cGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa              <  2:205989/151‑1 (MQ=255)                                                                                                                                         gAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          <  1:343465/151‑1 (MQ=255)                                                                                                                                           gggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt        <  2:270280/151‑1 (MQ=255)                                                                                                                                            ggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt       <  2:746541/151‑1 (MQ=255)                                                                                                                                                ggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa    >  1:402340/1‑150 (MQ=255)                                                                                                                                                  aTTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  >  1:522805/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                        CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC‑TCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑