Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,203	C→T	W36* (TGG→TGA)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,203	0	C	T	100.0%	277.6 / NA	71	W36* (TGG→TGA)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/32);  total (39/32)

TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATC  >  NC_000913/4635056‑4635349                                                                                                                                                    |                                                                                                                                                   atGAGAGTCGAAGCGGAATTGCAGCGCGATGTCCAGAATCGAACGCGCAGTCAGGCGCAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAAAAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCAc                                                                                                                                                 >  1:447523/2‑151 (MQ=255)  ggagaGTGGAAGCGGTATGGCAGCGCGATGTCCAAAAACGGCCGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACt                                                                                                                                                <  1:446193/150‑1 (MQ=255)   gagaGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACtt                                                                                                                                               >  2:288129/1‑151 (MQ=255)        tCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAAt                                                                                                                                          >  1:224355/1‑151 (MQ=255)          gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATa                                                                                                                                         >  2:564627/1‑150 (MQ=255)          gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATa                                                                                                                                         >  1:418275/1‑150 (MQ=255)          gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATa                                                                                                                                         >  1:385309/1‑150 (MQ=255)           aaGCGTTATTCCAGCGCGTTGCCCAGATCCGGACGCGCATTCAGGCGTAGTCCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACt                                                                                                                                                <  1:87828/142‑1 (MQ=255)            aGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAAcc                                                                                                                                      >  1:526975/1‑151 (MQ=255)             gCGGTATTGCAGCGCGATGTCCAAAAGCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCt                                                                                                                                     <  1:915201/151‑1 (MQ=255)              cGGCATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCt                                                                                                                                     >  1:372884/1‑150 (MQ=255)               gtttttccaggatgATGACCAGAAGGGTACGCGCAGTCGGCGGTGGGGCGGCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTg                                                                                                                                    <  2:83942/137‑1 (MQ=255)                  aTTGCAGCGCGATGTCCAGAACCGGACGCGCAGTCAGGCGTAGGGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCtt                                                                                                                                 <  1:301827/150‑1 (MQ=255)                        gcgcGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcgccgc                                                                                                                          >  1:212520/1‑151 (MQ=255)                        gcgcGATGCCCAGAATGGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCtt                                                                                                                                 <  1:191939/144‑1 (MQ=255)                            gATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTAc                                                                                                                       >  2:650702/1‑150 (MQ=255)                                 ccAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTc                                                                                                                 >  2:40316/1‑151 (MQ=255)                                   agaggcGGCCGCGCATTAAGGCGTAGCGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                                                                <  2:195816/145‑1 (MQ=255)                                   aGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGa                                                                                                               <  2:374691/151‑1 (MQ=255)                                     aaccGGACGCGCAGTCGGGGGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGc                                                                                                             <  1:519320/148‑1 (MQ=255)                                      aTCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCg                                                                                                            >  2:55649/1‑151 (MQ=255)                                                 aGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCTAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                                                                  >  1:904160/1‑150 (MQ=255)                                                   tCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATc                                                                                               >  2:825083/1‑151 (MQ=255)                                                    cAGGCGTGGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATc                                                                                               <  2:639330/150‑1 (MQ=255)                                                     aGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATcc                                                                                              >  2:630107/1‑150 (MQ=255)                                                      ggCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCa                                                                                             <  2:148715/150‑1 (MQ=255)                                                       gtgtagtaCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGa                                                                                           <  1:753678/149‑1 (MQ=255)                                                                gACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                                                                  >  1:651709/1‑151 (MQ=255)                                                                gACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                                                                  >  1:354285/1‑151 (MQ=255)                                                                gACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTc                                                                                   <  2:170766/150‑1 (MQ=255)                                                                 aCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                                                                  >  1:173655/1‑150 (MQ=255)                                                                  ccgccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                                                                  <  2:818288/149‑1 (MQ=255)                                                                   cgccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagc                                                                               >  2:139576/1‑151 (MQ=255)                                                                     ccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagcc                                                                              >  2:683837/1‑150 (MQ=255)                                                                          ttCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGAtt                                                                         >  1:31575/1‑150 (MQ=255)                                                                           tCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTa                                                                        >  1:737632/1‑150 (MQ=255)                                                                             gACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTaaa                                                                      >  1:505897/1‑150 (MQ=255)                                                                                aaaCGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                                                                  >  2:696537/1‑151 (MQ=255)                                                                                     acgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                                                              >  2:310368/1‑150 (MQ=255)                                                                                     acgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                                                              >  2:562050/1‑150 (MQ=255)                                                                                       gaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGACCGACAAGCGCTGATCCAGATGACCATCCAGCCAGATTACAAGGACGCGaat                                                           >  1:678562/1‑151 (MQ=255)                                                                                          cACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaataat                                                        <  1:141525/151‑1 (MQ=255)                                                                                           aCGAATATACGCGCCAATAGCATGTCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGTGCGGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaagaat                                                        >  1:161187/1‑150 (MQ=255)                                                                                           aCGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATg                                                       <  2:1210/151‑1 (MQ=255)                                                                                             gAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGcc                                                     >  1:66592/1‑151 (MQ=255)                                                                                               atatACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg                                                   >  2:85278/1‑151 (MQ=255)                                                                                               atatACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg                                                   >  1:75959/1‑151 (MQ=255)                                                                                                   aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt                                                <  1:165830/150‑1 (MQ=255)                                                                                                   aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt                                                <  1:7916/150‑1 (MQ=255)                                                                                                     gcgcCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt                                             >  1:187942/1‑151 (MQ=255)                                                                                                       gcCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt                                             <  1:128018/149‑1 (MQ=255)                                                                                                        ccAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCa                                          <  1:277794/151‑1 (MQ=255)                                                                                                         cAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCa                                          <  2:654787/150‑1 (MQ=255)                                                                                                          aaTAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCAt                                         <  2:134927/150‑1 (MQ=255)                                                                                                           aTAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATaa                                       <  2:372918/151‑1 (MQ=255)                                                                                                               cATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAat                                    >  2:37721/1‑150 (MQ=255)                                                                                                                 tGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATc                                 >  1:250938/1‑151 (MQ=255)                                                                                                                         gACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATcc                          <  1:331937/150‑1 (MQ=255)                                                                                                                         gACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCt                         <  2:742969/151‑1 (MQ=255)                                                                                                                          aCATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCtt                        >  2:707297/1‑151 (MQ=255)                                                                                                                              cTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTca                     >  2:431807/1‑150 (MQ=255)                                                                                                                                ttAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaac                   <  1:86313/150‑1 (MQ=255)                                                                                                                                ttAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTca                     <  2:570665/148‑1 (MQ=255)                                                                                                                                ttAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTca                     <  2:312983/148‑1 (MQ=255)                                                                                                                                 tAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaaca                  <  1:443968/150‑1 (MQ=255)                                                                                                                                      aTTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGc            >  2:190449/1‑151 (MQ=255)                                                                                                                                            tGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTg       >  1:660112/1‑150 (MQ=255)                                                                                                                                             gTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTg       <  2:102053/149‑1 (MQ=255)                                                                                                                                             gTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGa      <  2:224376/150‑1 (MQ=255)                                                                                                                                               aaGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACa    <  2:209636/150‑1 (MQ=255)                                                                                                                                                aGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATc  >  1:319050/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATC  >  NC_000913/4635056‑4635349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑