Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	47.6%	54.9 / 40.3	64	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/27);  new base T (30/0);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 4.12e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206291‑3206581                                                                                                                                                  |                                                                                                                                                   aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAc                                                                                                                                               >  1:147585/1‑150 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGAAAAATa                                                                                                                                          >  1:969666/1‑149 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                                        <  1:591294/151‑1 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCACAATATTTATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                         <  2:427757/150‑1 (MQ=255)         aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaa                                                                                                                                      <  1:42461/151‑1 (MQ=255)          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaaa                                                                                                                                     <  2:213311/151‑1 (MQ=255)             aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACt                                                                                                                                  <  1:965571/151‑1 (MQ=255)              ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAAAAAAACaaaaaa                                                                                                                                   >  2:713395/1‑148 (MQ=255)                   actCCCTGTTCTGGCGCAATATTCATTCTTATTTTCGCGTTAATTCACAAACTGTTAATTGCATATTCACGGTGCCAGAAAATATAAAACCACATTTATAGTGGTAGTTGGTGGCGGTG‑GATTTTTGCAGAAAAATACAAAAACTGGAGtt                                                                                                                            <  2:824272/149‑1 (MQ=255)                     tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                           <  2:407433/150‑1 (MQ=255)                      cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAAACAAAAAATgg                                                                                                                                >  2:493149/1‑144 (MQ=255)                      cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCATACAAATAAAAAAAATGGAGt                                                                                                                             >  2:928098/1‑147 (MQ=255)                         tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAACTGGAGtttgcat                                                                                                                       >  2:235472/1‑145 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                                     <  1:770075/151‑1 (MQ=255)                            tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACACATAaaaaaaatcgggtt                                                                                                                            >  2:3191/1‑134 (MQ=255)                             ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                    <  1:828001/149‑1 (MQ=255)                               ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                                <  1:23932/151‑1 (MQ=255)                                  gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                               >  1:379621/1‑149 (MQ=255)                                    aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATTGAGGTGCCATGATGAgggga                                                                                                             >  1:363786/1‑145 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGGGGAAGtaa                                                                                                        >  1:166211/1‑148 (MQ=255)                                           attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg                                                                                                    <  2:955440/151‑1 (MQ=255)                                           attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg                                                                                                    <  1:230099/151‑1 (MQ=255)                                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATTGGCGAAAGtaaaaa                                                                                                     >  1:112730/1‑149 (MQ=255)                                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAACAAAAAATGGAGTTGGCATGATGAGCGAAAGtaataa                                                                                                     >  2:924836/1‑149 (MQ=255)                                             tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAATAAAAAAAATGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                     >  1:183270/1‑148 (MQ=255)                                               ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAAATGG‑GGTGGCATGATGAGGGGAAGgaataaaaaa                                                                                                 >  2:647536/1‑146 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                              <  1:725292/151‑1 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTCCAGACAAATACAAAAACTGGGGTTGCCATGATGAGCGAAAGGAAAAAacaaca                                                                                               >  1:684611/1‑150 (MQ=255)                                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAGGTGGCATGATGAGCGAAAGTAATAAacaaca                                                                                               >  1:814154/1‑148 (MQ=255)                                                     tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                           <  2:958089/150‑1 (MQ=255)                                                       gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAAAAAAAAAAAAAAATGGAGTTGGCATGATGAGGGGAAGGAATAAACAAAAGGCAGTg                                                                                        >  2:598417/1‑151 (MQ=255)                                                         gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                      <  2:847304/151‑1 (MQ=255)                                                           tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                     <  1:302952/150‑1 (MQ=255)                                                            gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                    <  2:469749/150‑1 (MQ=255)                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGGGAAAAGAATAAAAAAAAAGCAGGGaa                                                                                      >  2:256140/1‑140 (MQ=255)                                                                      cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGGGGTGCCATGATGAGGGGAAGAAATAAACaaaaaggcgggaataa                                                                                   >  1:510662/1‑127 (MQ=255)                                                                         ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCCTGATGAGCGGAAGGAATAAACAACAGGCAGGGAATAAGTTGACag                                                                           >  1:659707/1‑146 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGGAAAAAGCAAAAAGCAGTGAATAAGTTGACAGAGAtttt                                                                    >  1:86251/1‑149 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                    <  1:11407/151‑1 (MQ=255)                                                                               aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGGTGCCCTGATGAGCGAAAATAATAAACAAAAGGCAGTGAAAAAATTGACAGAGATTGTCGCt                                                                >  2:658958/1‑151 (MQ=255)                                                                                cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                               <  2:518520/151‑1 (MQ=255)                                                                                cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAACAACAGGCAGGGAATAAATTGACAGAGAtt                                                                      >  2:122420/1‑144 (MQ=255)                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                            <  2:180484/151‑1 (MQ=255)                                                                                    ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAATACAAAAAATGGGGTTGCCATGATGAGGGAAAGTAATAAGCAAAAAGCAGTGAATAAAATGACAAAGATTGTGGCTAACtt                                                           >  2:474173/1‑151 (MQ=255)                                                                                      cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTCCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAAAAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAAttt                                                          >  1:107286/1‑150 (MQ=255)                                                                                         ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                      <  1:155897/151‑1 (MQ=255)                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGGGCGGAAGTAAAAAGCAACAGGGAGGGAATAAATTGACAGAGAATGTCGCTAAATTTAACGcc                                                    >  1:636235/1‑151 (MQ=255)                                                                                             gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAACAACAAGCAGGGAATAAAATGACAGAGATTGTCGCTAAATTTACCGcc                                                    >  1:110477/1‑149 (MQ=255)                                                                                                 aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTT‑AATTTTTTCAGACAAAAAAAAAAACTTGAGTTGGCATGGTGGGCGGAAGGAATAAACAACAGGCAGTGAAAAAGTTGACAGAGAATGTCGCTAACATTACCGcccagagt                                              >  2:977281/1‑145 (MQ=255)                                                                                                  aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAAAAAAAACTGGAGTTGGCATGATGAGCGAAAGTAAAAAACAAAAAGGAGTGAATAAATTGGAAGAAATTGTTGCTAACTTTTCccccccgaatt                                             >  2:541185/1‑144 (MQ=255)                                                                                                    atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAATGAGGTGGCATGATGAGCGAAAGAAATAAAAAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAAATTTAACCCCATGAttttt                                           >  1:970943/1‑149 (MQ=255)                                                                                                          aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTTCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGGTTACAGAGATTGTTGGTAACTTTACCCCCATGATTTTTAccc                                       >  2:782819/1‑149 (MQ=255)                                                                                                          aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                     <  1:891007/151‑1 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGGGGTGGCCTGATGGGCGGAAGGAATAAGCAACAAGCAGGGAATAAGTTGACAGAGAATGTCGCTAACTTTAACGCCCTGATTTTTACCCGaa                                    >  1:605433/1‑150 (MQ=255)                                                                                                                      aGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatga                          <  1:116770/150‑1 (MQ=255)                                                                                                                       gTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAAGGGGGTGCCATGATGAGCGGAAAGAATAAACAAAAAGCAGTGAAAAAGATGACAGAGAATTTCGGCAAAATTAACGCCCCGAATTTTTCCCGAAATCCtgc                             >  2:967180/1‑145 (MQ=255)                                                                                                                        tGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAATACAAAAACTGGGGGTGGCATGGTGAGCGAAAGTAATAAACAACAAGCAGGGAATAAAGTTAAAGAGAATGTCGCTCACTTTTCCGCCCTGATTTCTTCCCCAATGCCTGATGGCg                        >  2:420446/1‑150 (MQ=255)                                                                                                                              tttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAACCTGGGGTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTGAATAAGTTGACGGAGATTGCCGCTAACTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                  <  1:713473/150‑1 (MQ=255)                                                                                                                              tttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAGGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                  <  2:725700/150‑1 (MQ=255)                                                                                                                                 gtgGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa              <  1:519833/151‑1 (MQ=255)                                                                                                                                    ggggtgaaaTTTTCCCAGAAAAATAAAAAAACGGGAGTTCCAATGATGGGCAAAATAAAAAACCAACGGGCAGTAAAAAATTTGCCAGAGATTGCCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa               <  1:519868/141‑1 (MQ=255)                                                                                                                                      ggTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa          >  1:85314/1‑150 (MQ=255)                                                                                                                                           aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      <  2:213191/150‑1 (MQ=255)                                                                                                                                               tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  >  1:687743/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206291‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑