Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 73,581 | 0 | A | C | 47.5% | 90.4 / 15.0 | 59 | V314G (GTA→GGA) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base A (27/4); new base C (0/28); total (27/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.93e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC > NC_000913/73432‑73730 | tCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAc > 2:821352/1‑151 (MQ=255) cgcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg < 1:94462/151‑1 (MQ=255) gCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAt > 1:682409/1‑151 (MQ=255) gTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCAc > 2:199439/1‑151 (MQ=255) cgcgACTGCCCCATAGCAGCATCATGGTCAGCACCCCGCACAATACACCTGCCGCCAGCGCAATACGC‑ACCGAGGTCCACATCTCCTGCTACTGCACCGGTTGCGCCAGCACCTCCAGCAACTGGCGATTTACCCCATCGACGATCACCg < 2:375300/150‑1 (MQ=255) gACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGcc > 2:193253/1‑149 (MQ=255) cTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGt > 1:48466/1‑150 (MQ=255) ccAGAGCAGCATCATGGCCAGCCCTCCCCACAATCCCCCTGCCCCCGGCGCAATCCGC‑ACCGAGCCCCACAGCGCCTCCCACAGCACGGTTTGGGCCAGCACTCCCGGCACCTGGCGATTTCCCCCAGCGACGATCACCGCCAGTAAc < 2:291817/148‑1 (MQ=255) aTAGCACCATCAGGGTCACCCCAACCCACAATCCCCCGGCCGCCAGCGCAATACGC‑AACGGGGCCCACAGCGCCTGCCACAGCCCCGTTTGTGCCAGCCCTTCCGGCAACGGGGGATTTCCCCCATCGACGATCACCGCCAGTAACGGTg < 2:826517/150‑1 (MQ=255) cagcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGgcagc > 2:849427/1‑151 (MQ=255) agcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGgcagc > 1:583912/1‑150 (MQ=255) catcatGGTCAGCCCTCCCCACAATCCCCCTGCCGCCAGCGCAATCCGC‑AACGGGGTCCACAGCCCCTGCCACAGCCCCGGTTGCGCCAGCACTCCCGGCAACGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCaa < 2:15636/150‑1 (MQ=255) atcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAc > 1:654875/1‑150 (MQ=255) atcagGGTCACCCCTACCCCAAATCCCCCGGCCGCCAGCGAATTACGC‑AACGGGGCCCACAGCGCCTGCCACAGCCCCGGTTGGGCCACCACTTCCGGCAACGGGCGATTTCCCCCATCGCCGATCACCGCCAGTAACGGTGGCAGCAAc < 2:319814/150‑1 (MQ=255) tcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAc > 1:618893/1‑149 (MQ=255) gTCAGCCCTCCGCACAATCCCCCGGCCGCCAGCGAAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCCCGGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcagca < 1:674035/151‑1 (MQ=255) cACTACGCACAATCCCCCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCCCCGGTTGTGCCAGCACTTCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGcc < 1:74859/151‑1 (MQ=255) ctccGCACAATCCCCCCGCCGCCAGCGAAATCCGC‑AACGAGGCCCCCACCGCCTCCCACAGCCCGGGTTGTGCCACCCCTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAgcg < 2:339466/144‑1 (MQ=255) cccacaATCCCCCGGCCCCCAGCGAAACCCCC‑AACGAGCCCCACGGCCCCTCCCACAGCCCCGGTTGGGCCAGCCCTCCCGGCACCGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAg < 2:353377/146‑1 (MQ=255) cccaaaatcccccTGCCGCCGGCGCAATCCCC‑AACGGGGCCCACAGCGCCTGCCACAGCCCGGGTTGCGCCAGCCCTCCGGGCACCGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGc < 2:134811/138‑1 (MQ=255) cGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGcac > 2:848593/1‑151 (MQ=255) aaaatcccccTCCCGCCAGCGCAATCCGC‑ACCGAGGCCCACAGCGCCTGCCCCAGCCCCGGTTGGGCCAGCACTCCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACagcagcagc < 1:637300/132‑1 (MQ=255) aaacccccTGCCGCCGGCACAAT‑CGCAAAGGAGACCCACAGGGCCGGCCACGCCACGGGTTGGGCCAGCACTTCCGGAAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt < 2:632452/144‑1 (MQ=255) cacCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAacac > 1:148123/1‑151 (MQ=255) ccgccAGCGCAATCCGC‑AACGAGGCCCACAGCGCCTGCCACAGCCCCGTTTGTGCCAGCACTCCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTc < 1:817146/151‑1 (MQ=255) ccgccAGCGCAATACGC‑AACGAGGCCCACAGCGCCTCCCACAGCACCGTTTGGGCCAGCACTTCCGGAAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtgt < 1:337951/150‑1 (MQ=255) ccgccAGCGAAATACGC‑AACGAGGCCCACAGCGCCTGCCACAGCACCGGTTGGGCCAGCCCTTCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTc < 2:685076/151‑1 (MQ=255) gcgcAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAAt > 1:296955/1‑151 (MQ=255) aacaCGC‑AACGAGGCCCACAGCGCCTGCCACAGCACCGGTTGTGCCGGCCCTTCCGGAAACGGGCGATTCACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATg < 2:670852/145‑1 (MQ=255) aaTCCGC‑AACGGGGCCCACACCGCTTCCAACAGCCCCGGTTGGGCCAGCCCTTCCGGCAACGGGCGATTCCCCCCACCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATg < 2:69625/148‑1 (MQ=255) aaTACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCgg > 1:91978/1‑151 (MQ=255) aTACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGc > 1:184534/1‑151 (MQ=255) agcGAGGTCCACCGCGCCGGCCACAGCCCCGGTTGGGCCAGCACTTCCGGCAACGGGCGTTTTACCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATg < 1:731602/147‑1 (MQ=255) accGAGGCCCACGGCGCCTGCCACGGCCCGGGTTGGGCCAGCACTTCCGGCAACGGGCGATTTCCCCCACCGGCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTAt < 2:809186/146‑1 (MQ=255) aaCGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGc > 1:336368/1‑150 (MQ=255) gAGGCCCACAGCGCCTGCCACAGCACCGGTTGGGCCAGCACTTCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGAc < 1:821452/151‑1 (MQ=255) ggcccACAGCCCCTCCCACAGCCCCGGTTGGCCCAGCACTCCCGGCAAGGGGGGATTGCCCCCAGCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGc < 2:651556/142‑1 (MQ=255) ccccaGCCCCTCCCACAGCCCCGGTTGTGCCAGCATTCCCGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACg < 2:553126/144‑1 (MQ=255) caGCGCCGGCCACAGCCCCGTTTGTGCCAGCACTTCCGGCAACTGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGa < 2:668883/145‑1 (MQ=255) aGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCg > 1:442759/1‑151 (MQ=255) tGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcgc > 2:174488/1‑151 (MQ=255) tGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcgc > 2:121773/1‑151 (MQ=255) tGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcgc > 1:177719/1‑151 (MQ=255) ccccGGTTGTGCCAGCACTTCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGcc < 1:229943/148‑1 (MQ=255) cgggTTGGGCCAGCGCTTCCGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt < 1:107547/148‑1 (MQ=255) ccGTTTGTGCCCGCTCTCCCGGCAACGGGCGATTTCCCCCACCCACCATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt < 2:30258/150‑1 (MQ=255) gcgCCACCACTCCCGGCAACTGGCGATTCCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagca < 2:117830/148‑1 (MQ=255) tgCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCagcagc > 1:744129/1‑151 (MQ=255) gCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCg > 1:58016/1‑151 (MQ=255) ccGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg < 1:318504/151‑1 (MQ=255) cGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgc > 2:782046/1‑151 (MQ=255) cAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgcgc > 1:502844/1‑150 (MQ=255) cTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCCACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa > 2:848280/1‑149 (MQ=255) cTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATg > 2:331729/1‑151 (MQ=255) ggCGATTTCCCCCACCGACGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATg < 2:274925/149‑1 (MQ=255) ggCGATTCCCCCCACCGACGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAAt < 2:43928/148‑1 (MQ=255) gATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCtt > 2:61770/1‑151 (MQ=255) tttCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTa < 1:309456/150‑1 (MQ=255) tACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTc > 1:363895/1‑151 (MQ=255) | TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGC‑AACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC > NC_000913/73432‑73730 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |