Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7650GT42.1% 38.8 / 18.2 38Q249K (CAA→AAA) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base G (4/18);  new base T (16/0);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 2.25e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTG  >  NC_000913/646617‑646913
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aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCa                                                                                                                                                    <  2:527063/151‑1 (MQ=255)
 aGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCaa                                                                                                                                                   <  2:663783/151‑1 (MQ=255)
  gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCaat                                                                                                                                                  <  2:45604/151‑1 (MQ=255)
       tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGGCCCCTTTTTTCaataa                                                                                                                                                >  1:530211/1‑148 (MQ=255)
          ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTGaataaatttt                                                                                                                                           >  2:580187/1‑145 (MQ=255)
                aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAAGCCCCCCTTTTTTAATAAAGTTTTCGcc                                                                                                                                      >  1:170994/1‑149 (MQ=255)
                 aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTcg                                                                                                                                   <  1:417056/151‑1 (MQ=255)
                         cTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATGGCCCCCTTTTTCTATAATGTTTTCGcc                                                                                                                                      >  2:290151/1‑140 (MQ=255)
                                   ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCCTTTTTCAATAATATTTGCGCCTCGCGCTGTCGCCcgcg                                                                                                                     >  2:839232/1‑147 (MQ=255)
                                    gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGGCAGCGCAgg                                                                                                                 >  2:456835/1‑150 (MQ=255)
                                                    cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTGAATACCCCCCTTTTTTATTAATGTTTTCGCCTCCCGCCGTAGCCAGCGGCGGCCCCCCCCCCCCCcgcc                                                                                                >  2:177070/1‑150 (MQ=255)
                                                     aGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCTTTTTGCAATAATTTTTTCGCCTCCCGCCGTAGCCAGCGCAGGCCCCCCCCGCcccccc                                                                                                 >  2:394203/1‑147 (MQ=255)
                                                              gcttGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTTAATAAATTTTTGGCCTCGCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCGATGCCAc                                                                                       >  2:403977/4‑150 (MQ=255)
                                                                  ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTg                                                                                   <  1:834869/150‑1 (MQ=255)
                                                                  ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCTTTTTTTAATAATGTTTTTGCCCCGCGCCGTAGCCAGCGCAGGCCCCCCCCGCCACCCCGCGATGCCACGGTgg                                                                                  >  1:29107/1‑151 (MQ=255)
                                                                         gaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCGCGATGCAACGTTGGTATCGcc                                                                           >  1:588056/1‑151 (MQ=255)
                                                                            ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCTTTTTTCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCCGGCCCCCCTCGCCCCCCCGCGATGCAACGTTGGTATCGcc                                                                           >  1:237046/1‑148 (MQ=255)
                                                                                 tcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGCGGGTCGCATACCCCCTTTTTTGGATAAAGTTTTGGCCCCCCGCCGTGGACCGGGCCGGGCCCCCCCCCCCCCCCGCGATGCGACGGTGGTATcccc                                                                           >  1:73537/1‑143 (MQ=255)
                                                                                   gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGc                                                                  <  1:701495/150‑1 (MQ=255)
                                                                                    aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa                                                                <  2:585885/151‑1 (MQ=255)
                                                                                                  gAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCCATAATATTTTTGCCCCCCGCCGTAGCCAGCGCCGGCCCCCCCCGCCCCCCCCCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                                                         >  2:495583/1‑144 (MQ=255)
                                                                                                  gAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCTTTTTTTAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGATGCAACGTTGGTATCGCCGTTGATCGCCATCCGTAGGAg                                                      >  1:17497/1‑147 (MQ=255)
                                                                                                   aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAgg                                                 <  2:144809/151‑1 (MQ=255)
                                                                                                       gCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTag                                              <  1:78664/150‑2 (MQ=255)
                                                                                                       gCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATc                                             <  1:419046/151‑1 (MQ=255)
                                                                                                       gCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATc                                             <  2:177074/151‑1 (MQ=255)
                                                                                                        ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCACCCCGCGGTGCAACGGTGGGATCGCCGTTGATCGCCCTCAGTAGGAGGAAGGGATc                                             >  1:688761/1‑150 (MQ=255)
                                                                                                         cGGAGATAATCGAGATCGGCGGGGGGTCGAAAGCCCCCTTTTTTCAATAATGTTTTCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCACCCCGCCATGCAACGGTGGGATCGCCGGTTATCGCCCTCCGTAGGAGGaaagg                                                >  1:179386/1‑143 (MQ=255)
                                                                                                           gagaTAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc                                         <  2:228836/151‑1 (MQ=255)
                                                                                                                aaTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATg                                     <  1:547876/150‑1 (MQ=255)
                                                                                                                   cGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCa                                  <  1:56817/150‑1 (MQ=255)
                                                                                                                    gagaTCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAGGGCCCCCTCCCCACCCCGCCATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGGATCGAGCAATGCCAGt                                >  1:537383/1‑151 (MQ=255)
                                                                                                                       aTCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa                             <  1:833226/151‑1 (MQ=255)
                                                                                                                               gggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTaa                     <  1:330816/151‑1 (MQ=255)
                                                                                                                                  ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAccc                  >  1:730545/1‑151 (MQ=255)
                                                                                                                                         gCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc           >  2:454153/1‑151 (MQ=255)
                                                                                                                                         gCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATcc           <  2:784062/151‑1 (MQ=255)
                                                                                                                                           ccccTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcag         <  1:456884/151‑1 (MQ=255)
                                                                                                                                                    gCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTg  >  1:302428/1‑149 (MQ=255)
                                                                                                                                                    gCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTg  >  1:9686/1‑149 (MQ=255)
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AAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTG  >  NC_000913/646617‑646913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑