Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,981,720 G→A A223V (GCA→GTA)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,7200GA98.1% 193.1 / ‑2.8 52A223V (GCA→GTA) otsBtrehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/28);  minor base C (0/1);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTT  >  NC_000913/1981579‑1981865
                                                                                                                                             |                                                                                                                                                 
cAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCtgtg                                                                                                                                           <  2:76531/150‑1 (MQ=255)
     cGGTTAGATACTACGACAAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAAGGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAAt                                                                                                                                      <  2:294181/150‑1 (MQ=255)
        ttAGATACTACGACTAAACGACTCATAGTCATCCCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCTTTTCAAGCCAGCTCCAGACACCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAAtttt                                                                                                                                   <  2:766600/150‑1 (MQ=255)
        ttAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACCCCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAAtttt                                                                                                                                   <  2:540163/150‑1 (MQ=255)
         tAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAAttttt                                                                                                                                  <  1:135788/150‑1 (MQ=255)
                tacGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACAt                                                                                                                          >  2:83841/1‑151 (MQ=255)
                   gACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTcc                                                                                                                       >  1:436487/1‑151 (MQ=255)
                     cTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGc                                                                                                                     >  1:217980/1‑151 (MQ=255)
                       aaaCGACTCATAGTCATCACCCCTGTTTTTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACCCCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCg                                                                                                                      <  2:554952/148‑1 (MQ=255)
                           gACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTc                                                                                                               >  2:342529/1‑151 (MQ=255)
                           gACTCATAGTCATCACTCCGGTTATTTTCTCTTTTTTGTTGTATTGCGGTGGTTATCATTTAAAGCCACCTCAAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTc                                                                                                               <  1:187962/151‑1 (MQ=255)
                            aCTCATATTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTc                                                                                                               <  2:258932/150‑1 (MQ=255)
                                 tAGTCATCCCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGTTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGt                                                                                                            <  1:18367/148‑1 (MQ=255)
                                 tAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTCCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTa                                                                                                          <  1:34332/150‑1 (MQ=255)
                                   gTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACg                                                                                                       >  2:72501/1‑151 (MQ=255)
                                      atcaCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGa                                                                                                      <  1:394535/149‑1 (MQ=255)
                                      atcaCTCCTGTTATTTTCGCTTTTTTGTTGTAAGGCGGTGGTTATCATTTCAGGCCAGCCCCAGACATCCGGCGCGCCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATGCCGCGCAGTCGGTTAACGAc                                                                                                     <  2:339149/150‑1 (MQ=255)
                                       tcaCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGTTTTTCATTTCAAGCCAGCTCCAGACATCCGGCACCCCCGCCAGTCGCCATGACGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACg                                                                                                       <  1:286019/147‑1 (MQ=255)
                                       tcaCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTg                                                                                                   >  2:863052/1‑151 (MQ=255)
                                        caCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGc                                                                                                  >  1:22286/1‑151 (MQ=255)
                                        caCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGc                                                                                                  >  2:200420/1‑151 (MQ=255)
                                        caCTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACACCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACt                                                                                                    <  2:838465/149‑1 (MQ=255)
                                            ccTTTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAg                                                                                              <  1:62222/151‑1 (MQ=255)
                                            ccTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGaa                                                                                               <  1:491361/150‑1 (MQ=255)
                                            ccTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGa                                                                                                >  2:34357/1‑149 (MQ=255)
                                                  aTTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGcc                                                                                            >  1:98607/1‑147 (MQ=255)
                                                   tttcCTCTTTTTGGTGGAAATGCGGAGGTGACAATTTCAAGCCAGCTCCAAACCCCCGGCCCCCCCGCCAGTCGCCATGATGCCTGAGTTACGCCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGc                                                                                             <  2:757995/145‑1 (MQ=255)
                                                    tttCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGAtt                                                                                       <  1:510/150‑1 (MQ=255)
                                                      tctcTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCAt                                                                                    >  1:349875/1‑151 (MQ=255)
                                                        tctTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCg                                                                                  >  2:67911/1‑151 (MQ=255)
                                                         ctTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCAt                                                                                    <  1:196724/148‑1 (MQ=255)
                                                           tttttGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGtt                                                                               >  2:627482/1‑151 (MQ=255)
                                                              ttgttgTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTaa                                                                             >  1:218160/1‑150 (MQ=255)
                                                                  tgtAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAatcat                                                                        >  1:10973/1‑151 (MQ=255)
                                                                   gtAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAatcatc                                                                       >  2:559710/1‑151 (MQ=255)
                                                                      aTGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGcc                                                                    >  1:402746/1‑151 (MQ=255)
                                                                        gCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGccc                                                                   >  2:796520/1‑150 (MQ=255)
                                                                          ggtggtTATCATTTCAAGCCAGCCCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAg                                                                 <  1:632441/150‑1 (MQ=255)
                                                                               ttATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGaa                                                               <  1:700838/147‑1 (MQ=255)
                                                                               ttATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTACGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATAc                                                           >  1:656941/1‑151 (MQ=255)
                                                                                  tCATTTCAACCCAGCTCCAGACACCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACgg                                                         <  2:376183/150‑1 (MQ=255)
                                                                                       tCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc                                                   >  1:786914/1‑151 (MQ=255)
                                                                                         aacccAGCTCCAGCCATCCGGCACCCCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc                                                   <  2:851904/146‑1 (MQ=255)
                                                                                                    aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                      >  1:772834/1‑151 (MQ=255)
                                                                                                     gACACCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                      <  2:752148/150‑1 (MQ=255)
                                                                                                         tCCGGCACCCCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg                                    <  1:466908/148‑1 (MQ=255)
                                                                                                          ccGGCACACCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCt                                  <  2:703326/149‑1 (MQ=255)
                                                                                                               acacCCGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGc                            >  1:93850/1‑150 (MQ=255)
                                                                                                                  cccGCCAGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa                         <  2:768073/150‑1 (MQ=255)
                                                                                                                        aGTCGCCATGATGCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                  >  1:754766/1‑151 (MQ=255)
                                                                                                                                  atgCCTGAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTc        <  2:677763/151‑1 (MQ=255)
                                                                                                                                        gAGTTACACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCttt  <  2:566248/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGCGGTGGTTATCATTTCAAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTT  >  NC_000913/1981579‑1981865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑