Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,569	(A)8→7	coding (251/1686 nt)	ybjL ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,562	0	A	.	100.0%	266.9 / NA	55	coding (258/1686 nt)	ybjL	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/25);  total (30/25)

GACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCGAAAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGCCAATGGAATT  >  NC_000913/889412‑889705                                                                                                                                                       |                                                                                                                                                gACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCGa                                                                                                                                                 <  2:112190/151‑1 (MQ=255)           ggTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCGaaaaaaatgg                                                                                                                                        >  2:930174/1‑147 (MQ=255)                  aCGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGaaaaaa                                                                                                                                 >  1:816562/1‑149 (MQ=255)                  aCGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAg                                                                                                                               <  1:365047/151‑1 (MQ=255)                   cGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGt                                                                                                                              <  1:147293/151‑1 (MQ=255)                     tCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTc                                                                                                                            >  2:516903/1‑151 (MQ=255)                      cATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTc                                                                                                                            <  2:72087/150‑1 (MQ=255)                         agagCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTc                                                                                                                        >  1:657577/1‑151 (MQ=255)                            gCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCg                                                                                                                      <  1:242464/150‑1 (MQ=255)                                gCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCtt                                                                                                                  <  1:825749/150‑1 (MQ=255)                                      aTACCGGCCGTCAGGCCAATATCCCAGCCAATCAGCTTACCGAACCCTAAGGCGATCACCAGCGCAATGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGAcccc                                                                                                           <  1:851037/151‑1 (MQ=255)                                      aTACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGAcccc                                                                                                           <  1:103504/151‑1 (MQ=255)                                         ccGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGAc                                                                                                        <  1:818331/151‑1 (MQ=255)                                           ggCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGc                                                                                                      >  2:14553/1‑151 (MQ=255)                                           ggCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGc                                                                                                      >  1:875243/1‑151 (MQ=255)                                            gCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCa                                                                                                     <  2:519585/151‑1 (MQ=255)                                                  aGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAAt                                                                                               <  2:152844/151‑1 (MQ=255)                                                     ccAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATaa                                                                                             >  2:353381/1‑150 (MQ=255)                                                      cAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAAc                                                                                           >  1:57101/1‑151 (MQ=255)                                                          atCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGc                                                                                        >  2:188651/1‑150 (MQ=255)                                                          atCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCa                                                                                       <  1:183741/151‑1 (MQ=255)                                                            cccAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCAt                                                                                      >  1:332678/1‑150 (MQ=255)                                                                 ccAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGcc                                                                                <  2:792864/151‑1 (MQ=255)                                                                    aaCAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCa                                                                               >  1:495565/1‑149 (MQ=255)                                                                       aGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGAtt                                                                          >  1:653049/1‑151 (MQ=255)                                                                       aGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGAtt                                                                          >  2:463521/1‑151 (MQ=255)                                                                            aCCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAgc                                                                      <  2:162032/150‑1 (MQ=255)                                                                                aaCCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATc                                                                 >  1:162836/1‑151 (MQ=255)                                                                                 aCCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCg                                                                >  1:460035/1‑151 (MQ=255)                                                                                  cccTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCgg                                                               >  1:599354/1‑151 (MQ=255)                                                                                      aaGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGgtg                                                             <  2:741058/149‑1 (MQ=255)                                                                                       aGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTa                                                          <  1:330257/151‑1 (MQ=255)                                                                                           gATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGc                                                      >  1:524618/1‑151 (MQ=255)                                                                                              cACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGa                                                   <  1:320674/151‑1 (MQ=255)                                                                                               aCCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGa                                                   >  1:443750/1‑150 (MQ=255)                                                                                                ccAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGaa                                                  <  1:786387/150‑1 (MQ=255)                                                                                                    cgcACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAAtgtt                                             <  2:102850/151‑1 (MQ=255)                                                                                                         tGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTgg                                        >  1:23085/1‑151 (MQ=255)                                                                                                          gCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGttgttg                                         <  1:675710/149‑1 (MQ=255)                                                                                                          gCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTgg                                        <  2:206775/150‑1 (MQ=255)                                                                                                            cAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCt                                     >  2:721790/1‑151 (MQ=255)                                                                                                              aCCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCta                                    >  2:534065/1‑150 (MQ=255)                                                                                                                      cAGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCg                           >  2:705511/1‑151 (MQ=255)                                                                                                                       aGTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCg                           >  1:576687/1‑150 (MQ=255)                                                                                                                        gTGCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGAt                         <  1:705016/151‑1 (MQ=255)                                                                                                                          gCTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATAc                       >  2:630570/1‑151 (MQ=255)                                                                                                                           cTAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACg                      >  2:157831/1‑151 (MQ=255)                                                                                                                            tAACATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACg                      >  1:407121/1‑150 (MQ=255)                                                                                                                             aaCATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACg                      <  1:638161/149‑1 (MQ=255)                                                                                                                             aaCATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACg                      <  1:355299/149‑1 (MQ=255)                                                                                                                             aaCATTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGa                     <  2:232721/150‑1 (MQ=255)                                                                                                                                aTTAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTa                  >  2:415116/1‑150 (MQ=255)                                                                                                                                 ttAGGTAATTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTaa                 >  2:667071/1‑150 (MQ=255)                                                                                                                                       aaTTTTTCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGcc           >  2:147628/1‑150 (MQ=255)                                                                                                                                          ttttCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGCCAAt        <  2:146184/150‑1 (MQ=255)                                                                                                                                             tCCCATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGCCAATGGa     >  1:306279/1‑150 (MQ=255)                                                                                                                                               ccATCGCG‑AAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGCCAATGGAAtt  >  2:578636/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                GACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGATCACCAGCGCACTGCCAACCATCACCAGTGCTAACATTAGGTAATTTTTCCCATCGCGAAAAAAAATGGAAAAAAAGTTCGGTCCGGCTTCGACCCCGACGCAGAAAATAAACAGCATAAAGCCAAGATTAAGCGCATCGGTGTTAATGCTGAAATGTTGTTGGCCTAATAACAGCGATACGACTAAAACGCCAATGGAATT  >  NC_000913/889412‑889705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑