Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	47.0%	56.8 / 45.6	66	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/29);  new base T (31/0);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 3.98e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583                                                                                                                                                   |                                                                                                                                                      gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAg                                                                                                                                                    <  2:496578/150‑1 (MQ=255)    tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGa                                                                                                                                                   >  1:590458/1‑148 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAcca                                                                                                                                                >  2:26555/1‑146 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCCTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                             <  2:775874/151‑1 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATa                                                                                                                                             >  2:560888/1‑150 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaa                                                                                                                                          <  1:103175/151‑1 (MQ=255)            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCATACAAATaaa                                                                                                                                           >  2:615955/1‑146 (MQ=255)            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTAAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAATAaaaa                                                                                                                                         >  2:77752/1‑150 (MQ=255)             aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAacaaaacaaa                                                                                                                                         >  2:451184/1‑149 (MQ=255)               aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAaaaaaaaaaa                                                                                                                                       >  1:528427/1‑149 (MQ=255)                ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAcaaaaacaaaaa                                                                                                                                       >  2:355837/1‑148 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑g                                                                                                                                   <  2:504906/150‑1 (MQ=255)                  gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑gg                                                                                                                                  <  1:501379/150‑1 (MQ=255)                      ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATGCAAAAACT‑GGAGtt                                                                                                                              <  1:619831/150‑1 (MQ=255)                         ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGAATTTTTTTGACAAAAACAAAAACT‑GGAGTTTccc                                                                                                                          >  2:460007/1‑150 (MQ=255)                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAg                                                                                                                   <  2:266810/150‑1 (MQ=255)                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCTCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAg                                                                                                                   <  2:253053/149‑1 (MQ=255)                                   cgcAATATTCATTCTTATTTTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAAT‑GGAGGTGGCATGATGAGCg                                                                                                                 >  2:103951/1‑150 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAAT‑GGAGGTGCCATGGTGAGCGaa                                                                                                               >  2:636394/1‑150 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTAAATTTTTCAAAAAAATAAAAAAAAT‑AGAGTTGGCAAGATGTGGGaa                                                                                                               >  1:647455/1‑150 (MQ=255)                                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAATACAAAAAATAGGGGTTGCC‑TGATGAggggaa                                                                                                              >  1:576986/1‑143 (MQ=255)                                        tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGACAAAAAAAAAAAAT‑GGAGGTGCCATGATGAGGGaaa                                                                                                              >  2:815625/1‑148 (MQ=255)                                          ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGgaa                                                                                                          >  2:518388/1‑150 (MQ=255)                                            cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                       <  1:170164/151‑1 (MQ=255)                                               tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACT‑GGAGGTGCCCTGATGGGGGAAAGtaaaaa                                                                                                       >  1:592478/1‑148 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                   <  1:335530/149‑1 (MQ=255)                                                    ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAT‑GGGGGTGTCATGATGAGCGAAAGaaataaaaaaaa                                                                                                 >  1:68958/1‑143 (MQ=255)                                                         gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACAAAAATACAAAAAAT‑GGGGTTGGCCTGATGAGCGAAAATAATAAACAACAAGCAGTg                                                                                          >  1:595029/1‑151 (MQ=255)                                                          cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAAAAAAAAT‑GGGGGTTGCATGATGAGCGGAAGGAAAAAACaaaaag                                                                                               >  1:463000/1‑141 (MQ=255)                                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCGAAAAAAAAAAAAACT‑GGAGTTGCCACGATGAGCGAAAGTAATAAACAAAAGGCAGTGaa                                                                                        >  2:842976/1‑149 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  1:139023/150‑1 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  2:232795/150‑1 (MQ=255)                                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                  <  2:398358/150‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAAAAAAAAT‑GGAGGTGCCATGATGAGGGAAAGGAATAAGCAACAGGCAGGGAAAAAGTTGAc                                                                               >  2:879498/1‑150 (MQ=255)                                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                             <  2:339039/151‑1 (MQ=255)                                                                          gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATGCAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                           <  2:518422/149‑1 (MQ=255)                                                                              aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCACACAAATAAAAAAAAT‑GGAGGTTCCCTGATGAGGGGAAGGAAAAAACAAAAAGCAGTGAATAAATTGaaa                                                                              >  1:827188/1‑140 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                    <  1:379960/150‑1 (MQ=255)                                                                                  cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                 <  2:385908/151‑1 (MQ=255)                                                                                       tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAAAAAAAAT‑GGAGTTGGCATGATGAGCGAAAGTAATAAACAAAAAGGAGGGAATAAGTTGACAGAGAATGTTGCTAAAttt                                                            >  2:682345/1‑151 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAAAAAAAAT‑GGGGGTGCCATGATGAGCGAAAGGAATAAACAACAAGCAGTGAAAAAAATGACAGAGATTGTCGCTAACttt                                                            >  1:986783/1‑150 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                           <  2:114671/151‑1 (MQ=255)                                                                                              ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                      <  2:253686/150‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATACAAAAAAT‑GGGGTTGTCATGGTGAGCGAAAGGAATAAACAACAGGCAGAGAATAAGTTGACAGAGATTGTTGCTTACTTTAACCCCa                                                     >  1:898079/1‑148 (MQ=255)                                                                                                  aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAAT‑TGAGGTGCCATGGTGAGGGAAAGGAAAAAAAAAAAAGCAGGGAAAAAGTTGACAGAGATTTTCGCTTACTTTTCCGCCCTGa                                                  >  2:315281/1‑150 (MQ=255)                                                                                                     aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATACAAAAAAT‑GGAGGTGGCATGATGAGGGAAAGTAATAAAAAAAAGGCAGAGAATAAGATGACAGAGATTGTTGCTTAATTTACCCCCATGAtttt                                              >  1:487817/1‑150 (MQ=255)                                                                                                      atatAAAACCATATTTTAAGTGGTATTTTTTGGGGGTGATTTTTACCAGACATATCCAAAAATT‑GGGGTTGCAATGATGAACGAAAGTAATAAGCAACAGGCAGTGAAGAAGTTAACAGAAATTGGCGAATCCTGTCCCGCCATGATTTCt                                             <  1:212797/151‑1 (MQ=255)                                                                                                      atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                             <  2:623846/151‑1 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACAAAAAAA‑GGGGTTGGCATGATGAGGGAAAGTAATAAGAAAAAAGGAGGGAAAAAGATGAAAGAGATTGTTGCTAAATTTACCGCCATGAttttt                                             >  1:358751/1‑147 (MQ=255)                                                                                                           aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAT‑GGGGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAAGTGACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTAccc                                         >  2:652456/1‑150 (MQ=255)                                                                                                             aCCTCATTTTTAGTGGTATTTTGTGCCGGTGAATTTTTCCATCCAAATACAAAAGCG‑GGAGATGCCATGATGTGAGAAAGTAATAAGCAACAGGCAGTGAAGAAGTGGACCGGGATTGTGCCTATTTTTACCGCCATGATTTCTACCCGaa                                      <  2:483479/151‑1 (MQ=255)                                                                                                                  aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAAT‑GGAGGTGCCATGATGCGCGAAAGTAATAAACAACAAGGAGTGGAAAAGGTGACAAAGATTTTCGCTAACATTACCCCCATGATTTCTACCCGCAAGcc                                  >  2:295748/1‑150 (MQ=255)                                                                                                                     tttAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAAAAAAAAT‑GGAGGTGGCCTTATGGGGGGAAGGAATAAACAAAAAGCAGGGAATAAGTTTACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTTCCCCAAAGCCtgat                              >  2:157979/1‑151 (MQ=255)                                                                                                                      ttAGTGGTAGTTTGTGGCGGGGAATTTTTTCCGACCAAAACAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAAGCAGTGAATAAATTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCGCATGCCt                                 >  1:364996/1‑147 (MQ=255)                                                                                                                        aGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAACT‑GGAGGTGCCCTGATGGGCGGAAGGAATAAAAAACAGGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTTACCCCATGATTTTTACCCCAAAGCCtgat                              >  2:731268/1‑148 (MQ=255)                                                                                                                                tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                    <  2:350512/150‑1 (MQ=255)                                                                                                                                   gtgGCGGTGAATTTTTCCAGAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  1:249717/149‑1 (MQ=255)                                                                                                                                      gCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             >  2:855271/1‑151 (MQ=255)                                                                                                                                         gTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  1:70171/1‑150 (MQ=255)                                                                                                                                            aaTTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:355833/149‑1 (MQ=255)                                                                                                                                            aaTTTTTCCAGAAAAATACAAAAACT‑GGATTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAATTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:451179/149‑1 (MQ=255)                                                                                                                                            aaTTTTTCCAGAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        <  2:155436/150‑1 (MQ=255)                                                                                                                                               ttttCCAGAAAAATACAAAAACG‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     <  2:927054/150‑1 (MQ=255)                                                                                                                                                 ttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa   >  2:851064/1‑150 (MQ=255)                                                                                                                                                 ttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa   >  1:940204/1‑150 (MQ=255)                                                                                                                                                 ttCCAGACAAATACAAAAACG‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa   <  2:355005/150‑1 (MQ=255)                                                                                                                                                   ccAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:347146/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑