| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 718,659 | 0 | T | A | 42.5% | 57.9 / 16.5 | 40 | D601V (GAT→GTT) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base T (5/18); new base A (17/0); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAAC > NC_000913/718518‑718795 | gCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCAt < 2:772407/149‑1 (MQ=255) cATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTc < 2:62339/151‑1 (MQ=255) ccGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCt < 2:291186/150‑1 (MQ=255) ccGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTg < 1:850967/151‑1 (MQ=255) aGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGAcaca < 2:820540/151‑1 (MQ=255) gACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACaa < 2:691169/151‑1 (MQ=255) aCGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAAc < 2:947545/151‑1 (MQ=255) cACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGG‑ATAAAAAAAATGCCTTTTCTTACACAAATGACGCAggg > 1:471253/1‑149 (MQ=255) cACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGG‑AGACAAAAAAATCATTTTCTGACACAACTGACGCAcgggctaaac > 2:59703/1‑144 (MQ=255) cACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAAcc < 1:453700/151‑1 (MQ=255) ccGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCgg < 2:700221/151‑1 (MQ=255) aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAg < 2:329846/150‑1 (MQ=255) tAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGG‑GTACAAAACATGCATTTTCTTGCACAACTTACGCCGGGGATAAACcgcg > 1:848980/1‑138 (MQ=255) tAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAACATGCATTTCTTGGCACAACTGACGCAGGGGATAAACCGCGTAGCGCCCttttt > 2:354587/1‑148 (MQ=255) ggCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGt < 2:974868/151‑1 (MQ=255) ggATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAACATGCATTTCCTTACCCAACTGACGCCGGGGATAAACGGGGGAGCGGCCTTCTTGCTGTTTGTTGATgg > 2:49074/1‑151 (MQ=255) aTTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAACATGCATTTTCTTACACAACTTACGCAGGGGATAAACGGCGGAGCGCTCTTCATGCTGTTTGt > 2:848624/1‑143 (MQ=255) ttCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAAAATTCATTTCCTGACACAAAAGACGCAGGGGAGAAACCGCGGAGCGCTCTTCATTCTGTTTTTAGATgg > 1:439780/1‑148 (MQ=255) tCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGAC‑AGAGCAATCATGCATTTTCTCGAACAACTGTCGCCGGGGATAAAAGGCGGCGGGCTCTTCCTCATGCTTGGTGATGGAAg > 1:14844/1‑150 (MQ=255) cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAgg < 2:505775/150‑1 (MQ=255) gCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAACATGCATTTCCTGACACAAATGACGCAGGGGATAAACGGCGTAGCGCCCTTCATGCTGTTTTTAGAAGGAAGGTa > 2:98910/1‑149 (MQ=255) gACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCAc < 1:472172/150‑1 (MQ=255) cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTc < 2:270512/151‑1 (MQ=255) cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAACATGCCTTTTCTGACACAAATGACGCAGGGGATAAACCGCGGAGCGCTCTTCATTCTGTTTTTTGATGGAAGGGAGCACtt > 2:81832/1‑150 (MQ=255) gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑CTACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAACCCGCGCAGCGCCCTTCATGCTTTTTGTTGATGGGAGGGAGCACTTCt > 2:164716/1‑147 (MQ=255) gAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑GAATATAACATTCAATTACTGGCACAACAGGAGCAGGGGATAAACGGGGGAGCGCCCTTTCTTCTGTTTTTTGATGGAAggggggacctc > 1:239777/1‑135 (MQ=255) aaGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATAATTCATTTTCTTACACAACCGACGCAGGGGATAAACCGCGGAGCGCTCTTTATGCTGTTTGTAGATGGAAGGGAGGACtttttct > 2:928413/1‑142 (MQ=255) aGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGG‑ATACAAATCACGGATTTTCTGACCCAAATCACGCGAGGGATAAACGGCGGAGCGCCCTTCAACCTGTTTGTAGATGGAAGGGAGCACTTCTGCTaa > 2:11568/1‑148 (MQ=255) gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑GTACAAAAAATTTATTTTCTTGCCCAACATAAGCAGGGGATAAAACGCGTAGCGCTCTTCATTCTGTTTTTTGATGGGAGGGAGAACTTTTTCTaa > 1:934203/1‑145 (MQ=255) ccTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGcgcg < 1:305785/149‑1 (MQ=255) aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑CTACAAAACATGCATTTTCTTACACAACTGAAGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGTTTTTAGATGGAAGGGAGCACTTCTGCTAATGGCGGGTCgg > 2:613191/1‑149 (MQ=255) aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGa < 2:804875/150‑1 (MQ=255) aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑AGAAAAAACATTCCTTTTCTTACACAAATGACGCAAGGGATAAAACGCGTAGCGCTCTTCCTTCTGTTTTTTGATGGGAGGGAGGACTTCTTCTAATGGGGCGTCggg > 1:520584/1‑149 (MQ=255) ggAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑CGACAAAACATGTTTTTTTTTTCTCAACAGGCCCGGGGGATAACCGGACGGGCGCTCTTCACTATGCTTTTTGGTGGTAGGGATCACTTCTTCTTATAGGGCGGCGGGCTc > 1:195023/1‑151 (MQ=255) tctcTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAAATATTTATTTTCTTTCACAACTGACGCAGGGGATAAACGGCGGCGCGCTCTTTCTTCTGTTTTTTGAGGGGAGGGAGAACCTCTTCTCATGGCGCGTCGGGGTCAAGc > 1:16904/1‑149 (MQ=255) tGCAGTTGTTTCACGTTGTGGCGGGCGATA‑AAAACATTCATTTTCTTACAACAATGACGCAGGGGGTAAACGGAGTAGCGCTCTGTCTGTTTTTTTTTGATTGAAGGGAGGACTTCTGCTAATGGCGCGGCGGACCCCAACCGttttt > 1:419158/1‑148 (MQ=255) tGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCg < 2:545958/150‑1 (MQ=255) cAGTTGTTTCACGTTGTGGCGGGG‑ATTCAAATTATGCATTTTCTTGCACCACAGAGGCAGGGGAGAAACGGCGGAGCGCCCTTCATTCTGTTTGTTGTTGGGAGGGAGCACTTCTTCTTATTGCGCGGCGGGCCCCAGCAGgtttttgca > 1:100884/1‑146 (MQ=255) cACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACcag < 2:788249/150‑1 (MQ=255) ggCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaac > 2:427018/1‑151 (MQ=255) | GCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGC‑ATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAAC > NC_000913/718518‑718795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |