Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	814,029	T→G	D168E (GAT→GAG)	uvrB →	exision nuclease of nucleotide excision repair, DNA damage recognition component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	814,029	0	T	G	100.0%	192.8 / NA	53	D168E (GAT→GAG)	uvrB	exision nuclease of nucleotide excision repair, DNA damage recognition component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/20);  total (33/20)

TGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGATCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAACTGTTT  >  NC_000913/813882‑814176                                                                                                                                                    |                                                                                                                                                    tGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCa                                                                                                                                                   <  1:635091/150‑1 (MQ=255)        aTGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGcgcgcg                                                                                                                                            >  1:6916/1‑150 (MQ=255)             tttGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTCTCCGCGCTTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTg                                                                                                                                      >  1:359480/1‑151 (MQ=255)               tGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTgc                                                                                                                                     <  1:312469/150‑1 (MQ=255)               tGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTg                                                                                                                                      >  2:198556/1‑149 (MQ=255)                  ccGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCg                                                                                                                                 >  1:358257/1‑151 (MQ=255)                     ccaccaAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACt                                                                                                                              <  2:604026/151‑1 (MQ=255)                      caccaAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTg                                                                                                                             <  1:763727/151‑1 (MQ=255)                        ccaAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGc                                                                                                                           <  1:127201/151‑1 (MQ=255)                         caAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCg                                                                                                                          >  2:362694/1‑151 (MQ=255)                            aGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAg                                                                                                                       <  1:224990/151‑1 (MQ=255)                               gATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTg                                                                                                                    >  2:31856/1‑151 (MQ=255)                                     ggAGCGGCGTGATGTGGTTGTGGTGTCGTCTGTTTCCGCGATTTATGGTCTAGGCGATCCTGATTTAGATCTCAAGATGATGCTACATCTCGCGGTCGGTATGACTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCTGAGCTGCAATAc                                                                                                              <  1:697637/151‑1 (MQ=255)                                        gcggcgTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCt                                                                                                           <  2:677622/151‑1 (MQ=255)                                         cggcgTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTc                                                                                                          >  2:572172/1‑151 (MQ=255)                                           gcgTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTc                                                                                                          <  2:387262/149‑1 (MQ=255)                                            cgTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTa                                                                                                       <  2:49308/151‑1 (MQ=255)                                               gATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAAt                                                                                                     <  1:286998/150‑1 (MQ=255)                                               gATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATg                                                                                                    >  1:714905/1‑151 (MQ=255)                                                            tggCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTcc                                                                                        >  2:686490/1‑150 (MQ=255)                                                               cGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCg                                                                                    >  2:665980/1‑151 (MQ=255)                                                                gTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCg                                                                                    >  1:650108/1‑150 (MQ=255)                                                                gTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGt                                                                                   <  2:16440/151‑1 (MQ=255)                                                                gTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGt                                                                                   >  2:890570/1‑151 (MQ=255)                                                                      ttCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACt                                                                             >  2:282544/1‑151 (MQ=255)                                                                       tCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACtt                                                                            >  1:471968/1‑151 (MQ=255)                                                                       tCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGCTACtt                                                                            >  2:537303/1‑151 (MQ=255)                                                                           cgATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCcg                                                                        >  1:535632/1‑151 (MQ=255)                                                                            ggtttATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCTTCTGACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCTGACTGGCGGAGATGCAATACGCTCGTAATGATCAAGCATTCCAGGCTGGTAAtt                                                                            >  1:368963/3‑146 (MQ=255)                                                                                tATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTc                                                                   >  1:647144/1‑151 (MQ=255)                                                                                 aTGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCg                                                                  >  1:581412/1‑151 (MQ=255)                                                                                        gggCGATCCTGATTTATATCTCAAGATGCTGCTACATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGAAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCg                                                             >  1:836693/1‑149 (MQ=255)                                                                                          gCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt                                                         <  1:387109/151‑1 (MQ=255)                                                                                           cGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGGGCCGACTGGCGGAGCTGCAATCCGCTCGTAATGGTCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGAGGCGAGGTg                                                        >  1:477123/1‑151 (MQ=255)                                                                                                cTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgat                                                      >  1:510072/1‑148 (MQ=255)                                                                                                 tGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgat                                                      >  2:618720/1‑147 (MQ=255)                                                                                                   aTTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGatat                                                >  1:835213/1‑151 (MQ=255)                                                                                                       atatCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTc                                            >  1:313742/1‑151 (MQ=255)                                                                                                        tatCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc                                           >  2:883413/1‑151 (MQ=255)                                                                                                           ctcAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCgg                                        >  1:763068/1‑151 (MQ=255)                                                                                                               aGATGATGCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGTCGGAGCTGCAATACGATCGTAATGATAAAACATTCCAGCTTGGTACTTTCCGCGTTCGTCGCGCGGGGATAGATATCTTCCCgg                                        >  1:4480/1‑147 (MQ=255)                                                                                                                  tgatgCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGGGGCGAGGTGATAGATATCTTCCCGGCAGAAt                                  >  1:129968/1‑150 (MQ=255)                                                                                                                   gatgCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATc                                 <  1:785741/150‑1 (MQ=255)                                                                                                                   gatgCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATc                                 <  1:658299/150‑1 (MQ=255)                                                                                                                   gatgCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCg                                <  1:628824/151‑1 (MQ=255)                                                                                                                      gCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGAt                             >  1:563170/1‑151 (MQ=255)                                                                                                                      gCTCCATCTCACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGAt                             <  1:697814/151‑1 (MQ=255)                                                                                                                             ctcACGGTCGGTATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTg                      >  1:736944/1‑151 (MQ=255)                                                                                                                                 cggtcggtATGATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACt                  <  2:529880/151‑1 (MQ=255)                                                                                                                                           gATTATCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGaa        <  2:6916/151‑1 (MQ=255)                                                                                                                                               aTCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAACTg     <  2:695575/150‑1 (MQ=255)                                                                                                                                               aTCGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAACTGt    >  1:144569/1‑151 (MQ=255)                                                                                                                                                 cGAGCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGCGGCGAGGCGATAGATATCTTCCCGGCAGAATCGGATGAAATTGCACTTCGCGTGGAACTGttt  >  2:607332/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGAGCAGATGCGTTTGTCCGCCACCAAAGCGATGCTGGAGCGGCGTGATGTGGTTGTGGTGGCGTCTGTTTCCGCGATTTATGGTCTGGGCGATCCTGATTTATATCTCAAGATGATGCTCCATCTCACGGTCGGTATGATTATCGATCAGCGCGCGATTCTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAACTGTTT  >  NC_000913/813882‑814176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑