Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,900	0	A	G	42.9%	114.5 / 24.5	77	noncoding (72/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (3/41);  new base G (33/0);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.14e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAG  >  NC_000913/925755‑926044                                                                                                                                                  |                                                                                                                                                    tCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGtttg                                                                                                                                                 >  1:1740/1‑148 (MQ=255)        cGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCg                                                                                                                                         <  2:770583/151‑1 (MQ=255)         gTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAacccc                                                                                                                                          >  2:883865/1‑149 (MQ=255)          tACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTGCGataacccc                                                                                                                                         >  2:932018/1‑142 (MQ=38)           aCAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATa                                                                                                                                      <  1:1111471/151‑1 (MQ=255)            cAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAc                                                                                                                                     <  1:330242/151‑1 (MQ=255)            cAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAc                                                                                                                                     <  2:574248/151‑1 (MQ=255)             aGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGCGAACCCCCGATa                                                                                                                                      >  1:920232/1‑149 (MQ=255)             aGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACg                                                                                                                                    <  1:125462/151‑1 (MQ=255)              ggCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACg                                                                                                                                    <  1:895860/150‑1 (MQ=255)               gCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGtt                                                                                                                                  <  1:14287/151‑1 (MQ=255)                 aGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGTGGGGGGGGTTTGGAACCCCCCTACGtt                                                                                                                                  >  2:1109082/1‑149 (MQ=37)                  gCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGTTTGAAACCCCCATACGTTGcc                                                                                                                               >  1:1130425/1‑151 (MQ=255)                   cTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTATAAcccccccgaccgtggc                                                                                                                               >  1:718194/1‑139 (MQ=37)                    ttAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAACCCCGATACGTTGcc                                                                                                                               >  1:191228/1‑149 (MQ=255)                     tAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTCGGACCCCCGCTACGTTGCCGt                                                                                                                             >  2:862236/1‑150 (MQ=255)                       gAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGtata                                                                                                                          <  2:569743/151‑1 (MQ=255)                        aaaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAACCCCCATACGTTGCCGtat                                                                                                                           >  1:564120/1‑149 (MQ=255)                         aaTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca                                                                                                                        <  2:1160522/151‑1 (MQ=255)                          aTTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCCTAAGTTGCCGcat                                                                                                                           >  2:743425/1‑147 (MQ=25)                             cacaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacac‑ac                                                                                                                    <  1:749519/150‑1 (MQ=255)                              acaGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGGACCCCCCACACGGTGCCGTATaaac‑ac                                                                                                                    >  2:766928/1‑149 (MQ=25)                               caGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACtt                                                                                                                  <  2:565264/150‑1 (MQ=255)                                   tAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCATACGTTGCCGTATAAAC‑ACCTTcc                                                                                                               >  1:1029476/1‑149 (MQ=255)                                     aCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCGATACGTTGCCGTATACAC‑ACTTTTCacg                                                                                                            >  2:791764/1‑148 (MQ=255)                                     aCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGc                                                                                                           <  2:684310/151‑1 (MQ=255)                                     aCATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGc                                                                                                           <  1:261999/151‑1 (MQ=255)                                      cATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCCCTACGGTTCCGTATACAC‑AATTTCCCgg                                                                                                            >  1:663042/1‑149 (MQ=21)                                      cATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAAGTTGCCGTATACAC‑ACTTTCCAGGCg                                                                                                          <  1:628383/151‑1 (MQ=255)                                         aCTCCACCCGCCCACCATATTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGGAACCCCCCTACGTTGCCCTATACAC‑ACCTTcc                                                                                                               >  2:686206/1‑143 (MQ=21)                                          cTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGc                                                                                                       <  1:1202951/150‑1 (MQ=255)                                           tCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGTTCGCAACCCCGGTAAGTTGTCGTGTACAA‑ACATTTCATGCGTGCt                                                                                                      >  2:350530/1‑150 (MQ=21)                                                ccGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑Ca                                                                                               <  1:921461/151‑1 (MQ=255)                                                    ccaccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGGTCGAACCCCCCATACGTTGCCGTATACAC‑ACCTTCCCGGCGTGCTCCTT‑CAGcc                                                                                            >  2:37372/1‑150 (MQ=18)                                                     caccaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTCGGAACCCCCATAAGTTGCCGCATACACAACTTTCCA‑GCGGGGTCCTT‑CAGcc                                                                                            >  1:303277/1‑149 (MQ=18)                                                      accaTGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGACCCCCGATACGGTTCCGTATAAAC‑ACTTTTCAGGCGGGCTCCTT‑TAGcccc                                                                                          >  1:734746/1‑148 (MQ=18)                                                          tGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCGAAACGTTGCCGTATACAC‑ACCTTCCCGGCGGGGTCCTT‑CAGCCACCCgg                                                                                      >  1:933181/1‑150 (MQ=18)                                                             tCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGaca                                                                                   <  2:170386/150‑1 (MQ=255)                                                              cACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGacac                                                                                  <  2:161878/150‑1 (MQ=255)                                                                cTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGTTACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCt                                                                                <  2:955360/150‑1 (MQ=255)                                                                      tACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCGATACGTTGCCGTATACAC‑ACTTTTCCGGGGTGCTCCTT‑TCGCCACCCGGGCACCCCAccca                                                                          >  1:891684/1‑148 (MQ=17)                                                                          gacagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCCCCCC‑CAATTGt                                                                    >  1:739096/1‑151 (MQ=255)                                                                           acagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGtt                                                                   <  2:156320/151‑1 (MQ=255)                                                                            cagaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTGCCGTATACAC‑ACTTTTCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGttt                                                                  >  1:674255/1‑151 (MQ=255)                                                                              gaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCATACGTTGCCGTATACAC‑ACTTTCCAGGCGGGCTCCTT‑CAGCCACCCGGGCACCCCCCC‑AAAATGtttt                                                                 >  1:1103320/1‑150 (MQ=14)                                                                                 aaaaTGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGCTACGTTGCCGGATACAC‑ACTTTTCAGGCGGGGTCCTT‑TAGCCACTCGGGCACCCCACC‑AAAATTTTTTGCt                                                              >  1:734920/1‑150 (MQ=14)                                                                                      gCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCCAACGTTGCCCTATAAAA‑ACTTTTCCGGGGGGCTGCCT‑TTGCCACACGGACAACCCCCC‑AAATAGTTTTGCTAACaa                                                         >  2:389449/1‑150 (MQ=9)                                                                                         aaaaaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAAcc                                                      <  2:821020/150‑1 (MQ=255)                                                                                           aaaaaaGCTCGCACTTGCGTACGGGCTCTTCTTTAAAAATGGCCGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCCCC‑AAATTGTTTTGCTACCAAACCt                                                     <  2:1011178/149‑1 (MQ=255)                                                                                            aaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCAt                                                  <  2:548786/151‑1 (MQ=255)                                                                                               aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCTGACCCCCCCCACCGTTCCGGTTACAC‑CCTTTCCAGGCGTGCCCCTT‑CCCCCCCCCGGCCACCCCCCC‑CAAATGTTTTGCTTCCaaaaccccttgg                                               >  2:291528/1‑142 (MQ=9)                                                                                               aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAACCCCCCTACCTTTCCGTATAAAC‑ACTTTTCAGGCGGGCCCCTT‑CAGCCACCCCGACCCCCCCCC‑AAAATGTTTTTCTTCCAAACCTCCTggg                                               >  2:632970/1‑151 (MQ=14)                                                                                               aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATggg                                               <  2:182059/151‑1 (MQ=255)                                                                                               aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTTGAACCCCCCCTACGTTTGCGTTTAAAC‑ACCTTCCACGGGGGGTCCCT‑CCGCCACTCGGGAACCCCACC‑CAAATGTTTTTCTTCCAAACCCCCTggg                                               >  2:253366/1‑151 (MQ=9)                                                                                                aGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCCCTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGt                                              <  2:1115959/151‑1 (MQ=255)                                                                                                aGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCCATACGGTTCCGTATACAC‑ACTTTCCAGGCGTGCTCCCT‑CAGCCACTCGGACACCTCACC‑AAAATTTTTTTCTACCAAACCTCCTggg                                               >  1:101168/1‑150 (MQ=14)                                                                                                    cGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTgg                                            <  2:494550/149‑1 (MQ=255)                                                                                                     gCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCCAACCCCCCCTACGGTGCCCTTTACAC‑ACCTTTCAGGGGGGCCCCTT‑CAGCCACCCCGGCACCTCACC‑AAAATGTTTTTTTCCCaaaaccccttggggg                                            >  1:893898/1‑136 (MQ=2)                                                                                                      cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCATACGTTGCCGTATACAC‑ACTTTTCAGGCGGGCTCCCT‑CAGCCACCCGGGCACCCCACC‑AAAATTTTTTTCTACCCAACCCCATGGGGGGCaa                                         >  1:356188/1‑150 (MQ=14)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACgg                                      <  2:960964/149‑1 (MQ=255)                                                                                                           tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCCATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCCCC‑AAATTGTTTTTCTACCAAACCTCATGGGTGGCAACgggg                                    >  1:721812/1‑150 (MQ=255)                                                                                                                cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGctac                              <  1:542722/151‑1 (MQ=255)                                                                                                                  aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGctacta                            <  1:854435/151‑1 (MQ=255)                                                                                                                  aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCGACCAAACCTCATGGGTGGCAACGGGGCGctact                             <  2:945562/150‑1 (MQ=255)                                                                                                                   gCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTCGGACCCCCGCTTAGTTTTCCTATATAC‑AATTTTCCGGCGGGCGCCTTCCACCCCCCCGGAC‑CCCCACCAAAATTGTTTTTTTCCCAACCCCAATgggggtcacggg                                      >  2:899068/1‑132 (MQ=2)                                                                                                                        tcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAgg                        <  1:374901/149‑1 (MQ=255)                                                                                                                        tcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAg                         <  2:652266/148‑1 (MQ=255)                                                                                                                            tAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGtt                   <  2:260497/150‑1 (MQ=255)                                                                                                                                 atGGCGGTGAGGGGGGGATTCGCACCCCCGATACGTTGCCGTATAAAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCCCTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGGGGCAACGGGGCGCTACTATAGGGAGTTGGAGt              >  2:918112/1‑150 (MQ=255)                                                                                                                                 atGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTa             <  1:883888/151‑1 (MQ=255)                                                                                                                                   ggCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTaaa           <  1:758010/151‑1 (MQ=255)                                                                                                                                    gCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTaaaa          <  1:1053666/151‑1 (MQ=255)                                                                                                                                      ggTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACg        <  1:213864/151‑1 (MQ=255)                                                                                                                                      ggTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACg        <  2:1130395/151‑1 (MQ=255)                                                                                                                                          aGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCa    <  2:573323/151‑1 (MQ=255)                                                                                                                                          aGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCa    <  2:279129/151‑1 (MQ=255)                                                                                                                                            ggggggATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaag  <  2:356180/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACCCGCCCACCATGTTCACTGCCGTACAGACAGATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACAC‑ACTTTCCAGGCGTGCTCCTT‑CAGCCACTCGGACACCTCACC‑AAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAG  >  NC_000913/925755‑926044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑