Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	43.3%	104.4 / 17.5	60	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (2/32);  new base G (26/0);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 5.41e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097444‑1097729                                                                                                                                          |                                                                                                                                                       tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCg                                                                                                                                            <  2:1137170/150‑1 (MQ=37) tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCg                                                                                                                                            <  2:630749/150‑1 (MQ=37)         tGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTGCACCCCCCATACggt                                                                                                                                     >  2:1149720/1‑147 (MQ=11)          gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGtt                                                                                                                                     <  1:1134119/148‑1 (MQ=255)          gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGc                                                                                                                                   <  2:837377/150‑1 (MQ=255)            ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCg                                                                                                                                 <  1:519569/150‑1 (MQ=255)                 tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTTGGGGGGGGGTTTGGACCCCCCCTACGTTGGCGtcta                                                                                                                             >  1:296156/1‑149 (MQ=11)                         gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCATACGTTGCCGTATACACA‑Cttt                                                                                                                    >  1:5289/1‑149 (MQ=255)                         gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATAT‑GCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCa                                                                                                                 <  2:410345/151‑1 (MQ=255)                          ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCa                                                                                                                 <  1:1084177/151‑1 (MQ=255)                            aGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAgg                                                                                                               <  1:1181747/151‑1 (MQ=255)                               tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGc                                                                                                              <  1:975338/149‑1 (MQ=255)                                       cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTc                                                                                                    <  2:724797/151‑1 (MQ=255)                                        ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGTACCCCCGCAAAGATTCCGTTTATACA‑CTTTCCaacggggcctcat                                                                                                     >  1:787022/1‑137 (MQ=11)                                          ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTCGAACCCCCCGTACGTTGCCGTGTACACA‑CCTTTCAGGCGTGCTCCTTCacc                                                                                                 >  2:198825/1‑149 (MQ=17)                                          ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGc                                                                                                 <  1:170219/151‑1 (MQ=255)                                           gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGcc                                                                                                <  2:724845/151‑1 (MQ=255)                                             cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCa                                                                                               <  2:10512/150‑1 (MQ=255)                                              ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCACACGTTGCCGTATACACA‑CTTTTCCGGCGTGCTCCTTCAGcc                                                                                                >  1:1183849/1‑148 (MQ=255)                                              ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACt                                                                                             <  1:751843/151‑1 (MQ=255)                                              ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTTGTACCCCCCATAAGTTTTCGTAAAAACA‑CCTTCCAGGCCTgggccctccaccac                                                                                              >  1:809932/1‑137 (MQ=14)                                                 gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCATACGCTGCCGTATAAAAA‑CCTTTCCGGCGGGCTCCTTCAGCCACCCg                                                                                           >  2:448010/1‑150 (MQ=17)                                                 gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCgg                                                                                          <  1:1130131/151‑1 (MQ=255)                                                   ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTGCCGTATACACA‑CTTTTCAGGCGTGCTCCTTCAGCCCCTCgg                                                                                          >  1:658060/1‑149 (MQ=255)                                                       cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                                                    <  1:284249/151‑1 (MQ=255)                                                           ccccgGTTGTGTGAAATATCCAAAAAAAAGGCCCTTCCTTTCGTACGAGCTCTTCTTAAATTAGGGGGGGGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCa                                                                                  <  1:60727/149‑1 (MQ=14)                                                                ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAtt                                                                           <  2:1058884/151‑1 (MQ=255)                                                                   gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt                                                                         <  2:776177/150‑1 (MQ=255)                                                                    tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTTCCGGCGTGCTCCTTCCGCCCCCCCGACACCCCACCaaaat                                                                           >  2:738688/1‑145 (MQ=14)                                                                     gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                                                                       <  2:352650/150‑1 (MQ=255)                                                                       gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTcc                                                                  <  1:1182411/151‑1 (MQ=255)                                                                         aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCt                                                                 <  2:901566/151‑1 (MQ=255)                                                                          atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTg                                                                <  1:593373/151‑1 (MQ=255)                                                                            ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCCTACGTTTCCGTATAAACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCCCACCCAAATTTCG‑TTcc                                                                  >  1:1012457/1‑147 (MQ=14)                                                                                 aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTAGAACCCCCCATACGGTTTCGTATAAACA‑CTTTCCTGGCGTGCTCCCTCACCCCCCCGGGCACCACACCAAAATTTCgctgcctttct                                                             >  1:248661/1‑138 (MQ=2)                                                                                       aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCGCTAACTTCGCGTGAAAACA‑CTTTCCTGGCGGGTCCCTTTACCCCCCAGGCCCCCCCCCCAAATTGCTG‑TTCCCTTCTTCTTGGCACg                                                   >  1:106541/1‑151 (MQ=2)                                                                                            cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCATACGTTGCCGTATACACA‑CTTTCCAGGCGTGGTCCTTCAGCCACTCGGACACCTCACCCAATTGTCG‑TTCCTGTCTTTCTGGAACGGgcgc                                              >  2:904080/1‑151 (MQ=17)                                                                                              tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTTAGGGGGGGGTTTGAACCCCCCATCCTTTTCCGTATATACA‑CTTTCCCGGCGGGCTCCTTCCGCCACCCTGGCACCCCCCCCAATTTTCG‑TTTCTGTCTTTCTGGAAACGGCGCtt                                            >  2:816916/1‑150 (MQ=9)                                                                                               aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTa                                            <  1:681653/150‑1 (MQ=255)                                                                                                cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCGGGGGGGGTTTGTACCCCCCCTTCCTTTCCGTTTACACA‑CCTTTCCGGCGGGCGCCTTCCACCACCCGGGCACCCCACCAAATTTTCG‑TTCCTTTCTTTCTTGAAAggggg                                               >  2:951138/1‑144 (MQ=2)                                                                                                  ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAAt                                          <  1:1179710/149‑1 (MQ=255)                                                                                                   tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTTCCGTATACACA‑CTTTCCCGGCGGGCCCCTTCCGCCCCTCGGACACCCCACCAAATTGTCG‑TTCCTGTCTTTCTGGAACCGGCGCTAAatt                                        >  1:233334/1‑150 (MQ=17)                                                                                                    cGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCTAACCCCCCCTACGGTTCCGGATACACA‑ACTTTCAGGCGGGGTCCTTCACCCACCCGGGCCCCCCCCCCAAATTTCG‑TTCCTGTCTTTTTTGGACGGGGGCTAAttt                                        >  2:896936/1‑149 (MQ=9)                                                                                                      tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCTAACCCCCCAGACGGTTCCGTAAACACC‑CTTTTCAAGCGTGCGCCTTCACCCCCCCCGGCACCCCCCCCAATTGTCG‑GTCCCGTCTTGCTGGAAAGGGGGCTAATTTTggg                                    >  1:30783/1‑151 (MQ=9)                                                                                                      tACGAGCTCTTCTTTAAATATGGCGGTAGCGGGGGGGGTCCAAACCCCGCAACGTTGGCCGATTCACC‑CATTTCAAGCGTGGTCCCTTTGCCACCCCGACACCTCCCCCAATTTTCGTTTCTTTTCTTCCTGG‑ACGGGGGGTAAtttcagg                                    >  1:674026/1‑147 (MQ=2)                                                                                                         gAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGGTTGAACCCCCCCTTCGTTTCCCTATACACA‑CCTTTCAGGCGGGCTCCTTCAGCCACCCCGACACCTCACCAAAATGTCG‑TTCCTGTCTTTCTGGAACGGGCGCCAATTTAgggga                                  >  2:953900/1‑148 (MQ=17)                                                                                                          aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCCCTCGGACACCTCCCCAAATTTTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaaa                                 >  1:75400/1‑150 (MQ=255)                                                                                                            ctctTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAACCCCCATACGGTTCCGTATACACA‑CTTTTCCGGCGTGCTCCTTCAGCCACTCGGGCACCTCACCAAATTTTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaaa                                 >  1:261556/1‑148 (MQ=18)                                                                                                               ttcttTAAATATGGCGGTGAGGGGGGGGTTCGGACCCCCCGTTTGTAGCCGTTTAAAGACCTTTCCCGG‑GTGTTCCTTCAGCCACCCCGCCACCCCCCCAAAATTGCG‑CTCCCGTCTTTGTGGGACAGGCGCCAAttttatgaaaaaatg                            >  2:361622/1‑138 (MQ=2)                                                                                                               ttcttTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTTCCGCATCAACA‑CTTTCCAGGCGTGCCCCTTCAGCCCCCCGGCCACCTCCCCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGGGCTAATTTTGGGaaa                                 >  1:686473/1‑145 (MQ=17)                                                                                                                tcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCAt                             <  2:994086/148‑1 (MQ=255)                                                                                                                 cttTAAATATGGCGGTGAGGGGGGGGTTCGCAACCCCCATACGTTGCCGTATACACA‑CTTTTCACGCGTGCTCCTTCAGCCACTCGGACACCCCACCAAATTTTCG‑TTCCTGTCTTGCTGGGAAGGGGGCTCATTTTGGGAAAACATGAcc                         >  1:426871/1‑151 (MQ=17)                                                                                                                           ggCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac               >  2:77191/1‑151 (MQ=255)                                                                                                                           ggCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaa                <  2:1075028/150‑1 (MQ=255)                                                                                                                              ggTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACaaa            <  2:1207135/151‑1 (MQ=255)                                                                                                                                  aGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt        <  1:242643/151‑1 (MQ=255)                                                                                                                                  aGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt        <  2:1143214/151‑1 (MQ=255)                                                                                                                                   gggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt       <  2:1068442/151‑1 (MQ=255)                                                                                                                                    ggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt      <  2:964918/151‑1 (MQ=255)                                                                                                                                         gATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAGCGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaa  >  2:963093/1‑150 (MQ=255)                                                                                                                                          |                                                                                                                                                       TCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG‑TTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097444‑1097729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑