Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,039	0	T	G	50.0%	82.1 / 15.7	62	N108T (AAC→ACC)	yphE	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (10/21);  new base G (31/0);  total (41/21)
Fisher's exact test for biased strand distribution p-value = 4.82e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGG  >  NC_000913/2677894‑2678179                                                                                                                                                   |                                                                                                                                             ctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGGCAGA‑GGgtt                                                                                                                                            >  2:977068/1‑148 (MQ=255)  tgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTcc                                                                                                                                         >  1:3371/1‑150 (MQ=255)   gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCACCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGc                                                                                                                                       <  2:41142/151‑1 (MQ=255)   gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGgttttc                                                                                                                                         >  1:798196/1‑147 (MQ=255)    ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGGCGA‑GGGGTTTCGcc                                                                                                                                      >  1:858835/1‑151 (MQ=255)      tttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGcc                                                                                                                                      <  1:356564/149‑1 (MQ=255)          cgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCTACCGAGGCAAAGGGTTTTCcccc                                                                                                                                      >  2:1091954/1‑146 (MQ=255)           gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGGAGA‑GGGTTTTCGCCCCTGt                                                                                                                                 >  2:559680/1‑149 (MQ=255)            ccGGGCTTAGCGTTGAACCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTc                                                                                                                                <  2:438791/149‑1 (MQ=255)            ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCAGA‑GGGTTTTCGCCACTGt                                                                                                                                 >  1:39344/1‑148 (MQ=255)                 cTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTTCCCCACCGTCAGCCCtt                                                                                                                         >  1:446557/1‑151 (MQ=255)                    aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGGAGA‑GGGTTTTCGCCCCCGTCAGCCCCTcc                                                                                                                       >  2:1074986/1‑150 (MQ=255)                        ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCAGA‑GGGTTTCCCCCACTGTCAGCCCCTccacc                                                                                                                    >  1:1174454/1‑149 (MQ=255)                                aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTTCGCCCCCGTCAGCCCTTCCACCCGACtt                                                                                                              >  1:954859/1‑147 (MQ=255)                                aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTTCCCCACTGTCAGCCCCTCCCCCACACTTaa                                                                                                            >  1:32728/1‑149 (MQ=255)                                 gttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGAGGCCCCGGCCGCGGGAGG‑GGTTTTTCGCCCCCGTTGGCCCCTCCCCCAACCTTAACt                                                                                                          >  2:1137076/1‑150 (MQ=255)                                    gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCt                                                                                                       <  2:593215/150‑1 (MQ=255)                                    gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCt                                                                                                       <  1:651978/150‑1 (MQ=255)                                     ttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTCCGCCACTGTCAGCCCCTCCACCAGACTTTACTcc                                                                                                        >  1:184098/1‑148 (MQ=255)                                      tCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTTCGCCACTGTCAGCCCTTCCCCCAGAATTAACt                                                                                                          >  1:974775/1‑145 (MQ=255)                                           aCTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAcc                                                                                               <  1:864937/151‑1 (MQ=255)                                             tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCGAGGGAGA‑GGTTTTTCCCCACCGTCAGCCCCTCCACCAGACTTAAATCCCGATaaaaccc                                                                                             >  1:372130/1‑147 (MQ=255)                                             tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGGCAGA‑GGGTTTTCGCCCCTGTCAGCCCCTCCCCCAGACTTAACTCCTGATAAAAcgc                                                                                             >  1:917155/1‑151 (MQ=255)                                               aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACcgcgc                                                                                           <  1:164552/151‑1 (MQ=255)                                                    aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGCAGA‑GGGTTTTCGCCCCCGTCAGCCCCTCCCCCCCACTTAACTCCTGATAAACCCCGCGCAcc                                                                                      >  2:252672/1‑151 (MQ=255)                                                       gCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCAGA‑GGGTTTTCGCCCCCGTCAGCCCTTTCACCCGACTTAACTCCTCATAAACCCCGCGCAccccc                                                                                   >  2:50879/1‑151 (MQ=255)                                                          cAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCCACCGCGGGAGA‑GGGTTTCCCCCACTGTCAGCCCCTCCCCCCGACTTAACTCCCGATAAAACCCGCGCcccccc                                                                                   >  2:208225/1‑148 (MQ=255)                                                                    aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGAACGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTTACTCCTGATAAAACCCGCGCACCCCCCGTTCAGCGGc                                                                        >  1:735081/1‑149 (MQ=255)                                                                    aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCCGA‑GGGTTTTCGCCCCCGTCAGCCCCTCCACCCGACTTAAATCCTGATAAAACCCGCGCACCCCCCGATCAGCGGCAc                                                                      >  2:736897/1‑151 (MQ=255)                                                                    aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCAGA‑GGGTTTTCGCCCCCGTCAGCCCCTCCACCCAACTTAACTCCTGGTAAAACCCGCGCAaccccc                                                                                  >  2:973837/1‑138 (MQ=255)                                                                     aGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg                                                                     <  2:181241/151‑1 (MQ=255)                                                                           gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGGAGA‑GGGTTTCCGCCACTGTCAGCCCTTCCCCCCGACTTAACTCCTGATAAACCGCGCGCACCCCCCCTTCAGCGGCACGGCCAg                                                                >  1:364784/1‑150 (MQ=255)                                                                           gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGt                                                               <  1:1189193/151‑1 (MQ=255)                                                                            ttGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGt                                                               <  1:756470/150‑1 (MQ=255)                                                                             tGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGCGGA‑GGGTTTTCGCCACCGTCAGCCCTTCCCCCCGACTTTACTCCTGATAAAACCCGCGCCCCCCCAGCTCCACGGCACGGCggg                                                                >  2:812246/1‑146 (MQ=255)                                                                               ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCACCGAGGCAGA‑GGGTTTTCGCCACCGTCAGCCCCTCCCCCAGACTTAACTCCTGATAAAACGCGCGCCCCCCCCGCTCAGCGGCCCGGCGAGGCAGc                                                           >  2:1074659/1‑151 (MQ=255)                                                                               ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGc                                                           <  1:660085/151‑1 (MQ=255)                                                                                  aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCACCCGGGGGGA‑GGGTTTTCGCCCCTGTCCGCCCCTCCCCCAGACTTTACTCCTGATAAAACGCGCCCCCCCCCCGTTCAGCGGCACCGCGGGTCcgcg                                                          >  2:25567/1‑149 (MQ=255)                                                                                   tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTa                                                        <  1:728712/150‑1 (MQ=255)                                                                                     cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCAGA‑GGGTTTTCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGCTCAGCGGCCCGGCGAGGCAGCGTAg                                                       >  2:378515/1‑149 (MQ=255)                                                                                        cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAATCCTGATAAACCGCGCGCACCCCCACTTCAACGGCACCGCGAGTCAGCGTAGCTTc                                                   >  1:1029855/1‑150 (MQ=255)                                                                                          ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGGCGA‑GGGTTTTCGCCACTGTCAGCCCTTCCCCCCGACTTAACTCCCGATAAAACCCCCGCACCCCCCGTTCAGCGGCCCGGCGGGGCCGCGGAGCtttg                                                  >  2:1025455/1‑147 (MQ=255)                                                                                          ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTc                                                <  2:801062/151‑1 (MQ=255)                                                                                          ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTc                                                <  1:220791/151‑1 (MQ=255)                                                                                          ccATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTc                                                <  2:777238/151‑1 (MQ=255)                                                                                               tGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGAACGAGGCAGA‑GGGTTTTCCCCACTGTCAGCCCCTCCCCCCCACTTTAATCCTGATAAAACGCCCGCACCCCCCATTCAGCGGCACGGCGAGTCAGCGGAGCTTTGTCTcc                                             >  1:49986/1‑149 (MQ=255)                                                                                                  aGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTcc                                         <  2:111821/150‑1 (MQ=255)                                                                                                  aGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGGGGGAGA‑GGGTTTTCCCCCCTGTCAGCCCCTCCACCCGACTTAACCCCTTATAAAACCCGCGCCCCCCCCGTTCCGCGGCACGGCGGGGCAGCGTAGACTCGTCACCCTccc                                        >  2:1130562/1‑150 (MQ=255)                                                                                                    gTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGGCGA‑GGGTTTTCGCCCCTGTCACCCCCTCCACCCGACTTAACTCCCGATAAACCCCGCGCACCCCCCGGTCCGCGGCACGGCGCGTCAGCGTAGCTTTGTCCCCCTcc                                         >  2:418982/1‑148 (MQ=255)                                                                                                      aatcaatcATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc                                     <  1:556716/150‑1 (MQ=255)                                                                                                      aatcaatcATGCCGTTGCGGCGGGGCCACTGAACGAGGGGGA‑GGGTTTTCGCCCCTGTCAGACCTTCCACCACACCTTACCCCTGCTAAACAACGCGCACCCCCCGTTCAGCGGGACGGCGCGGCAGCGGAGCTTCGTCACCTTCCCGTc                                     >  2:793515/1‑150 (MQ=255)                                                                                                          aatcaTGCCGTTGCGGCGGGGCCCCCGACCCCGGCAGA‑GGGTTTTCGCCCCCGTCCGCCCCTCCACCCAACCTAACTCCTGGTAAAACGCGCCCACCCCCCCTTCAACGGGACGGCGGGGTAGCGGAACTTCGTCACCCTCCCGTc                                     >  2:926319/1‑146 (MQ=255)                                                                                                             caTGCCGTTGCGGCGGGGCCACTGACCGCGGCAGA‑GGGTTTTCGCCCCTGTCAGCCCCTCCACCCGACTTAACTCCTGATAAACCCCCCGCACCCCCCGTTCAGCGGCCCGGCGAGTCAGCGTAGCTTCGTCACCCTCCACTCGCGgctc                              >  2:1059724/1‑150 (MQ=255)                                                                                                               tGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTTCGCCCCTGTCAGCCCCTCCACCAGACCTAACTCCCGATAAAACCCGCGCACCCCCCGTTCCGCGGCACGGCGGGGCAGCGGAGCTTCGTCACCTTCCCGTCGCGTCTCGcc                           >  2:206116/1‑151 (MQ=255)                                                                                                                  cGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAAt                        <  1:1076052/151‑1 (MQ=255)                                                                                                                      gcggcgGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCa                     <  2:1108079/150‑1 (MQ=255)                                                                                                                      gcggcgGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCa                     <  1:559696/150‑1 (MQ=255)                                                                                                                       cggcggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATcc                      >  1:298072/1‑148 (MQ=255)                                                                                                                         gcggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGata                 <  2:578661/151‑1 (MQ=255)                                                                                                                         gcggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGat                  >  2:957055/1‑150 (MQ=255)                                                                                                                          cggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat                >  2:770101/1‑151 (MQ=255)                                                                                                                                cACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGc          >  1:1179352/1‑151 (MQ=255)                                                                                                                                     aCCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATcc     >  1:61806/1‑151 (MQ=255)                                                                                                                                        gAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCggg  >  2:287131/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                             CTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGG  >  NC_000913/2677894‑2678179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑