Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,823	C→A	intergenic (‑55/+11)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,823	0	C	A	100.0%	324.7 / NA	83	intergenic (‑55/+11)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/34);  total (49/34)

TTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCG  >  NC_000913/3815680‑3815968                                                                                                                                                  |                                                                                                                                                   ttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTa                                                                                                                                              >  1:32396/1‑150 (MQ=255)     gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAAt                                                                                                                                         >  2:349622/1‑151 (MQ=255)          gATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGtt                                                                                                                                    >  1:940401/1‑151 (MQ=255)          gATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGtt                                                                                                                                    <  1:890275/151‑1 (MQ=255)           aTTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGttt                                                                                                                                   >  1:757946/1‑151 (MQ=255)                 gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAgc                                                                                                                             <  1:1174039/151‑1 (MQ=255)                   gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAgcgc                                                                                                                           <  1:552603/151‑1 (MQ=255)                     aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGcgccg                                                                                                                         <  2:127696/151‑1 (MQ=255)                          cGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                                                                                                                    >  2:582655/1‑151 (MQ=255)                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                   >  2:873420/1‑151 (MQ=255)                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                   <  1:416636/151‑1 (MQ=255)                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                   <  2:787154/151‑1 (MQ=255)                           gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                                   <  1:721180/151‑1 (MQ=255)                             cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                                                 <  1:416179/151‑1 (MQ=255)                             cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                                                 <  2:808403/151‑1 (MQ=255)                               aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                               <  2:294333/151‑1 (MQ=255)                                     aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                                                                                          <  1:43833/150‑1 (MQ=255)                                      actccTGCTTGTTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTGGCCAGCGCCGCCTTCTGCGTCGCTACa                                                                                                        <  2:600319/148‑1 (MQ=255)                                        acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                      >  1:240297/1‑151 (MQ=255)                                        acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                      <  1:175022/151‑1 (MQ=255)                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                                      <  1:926197/150‑1 (MQ=255)                                         ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                                                                     >  1:784203/1‑151 (MQ=255)                                          cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATgg                                                                                                    >  2:884455/1‑151 (MQ=255)                                            gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑a                                                                                                  <  2:675174/150‑1 (MQ=255)                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑attcg                                                                                              >  1:1065406/1‑150 (MQ=255)                                                gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTGCTACAGAC‑AAAAAAAAGGCGACGCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑attcg                                                                                              <  2:228154/150‑1 (MQ=255)                                                 ctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑attcgat                                                                                            >  1:174577/1‑151 (MQ=255)                                                  tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑attcgatt                                                                                           >  1:1161046/1‑151 (MQ=255)                                                   aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGg‑attcgat                                                                                            <  1:427276/149‑1 (MQ=255)                                                        cAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTc                                                                                     <  2:444194/151‑1 (MQ=255)                                                        cAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTc                                                                                     >  1:24181/1‑151 (MQ=255)                                                         aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCg                                                                                    <  2:17885/151‑1 (MQ=255)                                                           aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCggg                                                                                  <  1:270073/151‑1 (MQ=255)                                                             tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGcc                                                                                >  2:1163090/1‑151 (MQ=255)                                                             tCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGcc                                                                                >  1:117092/1‑151 (MQ=255)                                                              cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGcc                                                                                >  2:1015/1‑150 (MQ=255)                                                               aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCag                                                                              <  2:919392/151‑1 (MQ=255)                                                               aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCCACTCATTCAG‑AACCTTAAAAATCAAGTTTCCAACCCCGCCCTCTTCGGCGCCAACATGGAATCCGATCCCCCCCggcc                                                                                 >  1:585067/1‑147 (MQ=255)                                                                aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGcc                                                                                >  2:1005768/1‑148 (MQ=255)                                                                 tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCag                                                                              >  2:252580/1‑149 (MQ=255)                                                                  aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCagag                                                                            <  1:1206814/150‑1 (MQ=255)                                                                    aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGcc                                                                          >  2:447152/1‑150 (MQ=255)                                                                    aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGcc                                                                          >  1:1072397/1‑150 (MQ=255)                                                                    aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGcc                                                                          >  1:325655/1‑150 (MQ=255)                                                                         cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAg                                                                    <  1:453121/151‑1 (MQ=255)                                                                            tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                                 >  1:1063103/1‑151 (MQ=255)                                                                             gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa                                                                <  2:891839/151‑1 (MQ=255)                                                                               tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                              >  1:1114640/1‑151 (MQ=255)                                                                                    tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                         >  2:121523/1‑151 (MQ=255)                                                                                      tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctct                                                        <  1:124248/150‑1 (MQ=255)                                                                                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTc                                                      >  2:1069590/1‑150 (MQ=255)                                                                                          gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                    <  2:151663/150‑1 (MQ=255)                                                                                          gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                   >  1:524120/1‑151 (MQ=255)                                                                                          gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                   >  2:416160/1‑151 (MQ=255)                                                                                           ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                   >  1:957515/1‑151 (MQ=255)                                                                                              tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                               >  2:206753/1‑151 (MQ=255)                                                                                                gCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                              >  2:388692/1‑150 (MQ=255)                                                                                                   cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                            <  2:426335/149‑1 (MQ=255)                                                                                                   cctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                          <  2:1215816/151‑1 (MQ=255)                                                                                                    ctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                         >  2:380497/1‑151 (MQ=255)                                                                                                      cATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                        <  2:1007072/150‑1 (MQ=255)                                                                                                         cTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                    >  1:535590/1‑151 (MQ=255)                                                                                                           tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                      <  2:782678/147‑1 (MQ=255)                                                                                                           tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                  >  1:425521/1‑151 (MQ=255)                                                                                                           tACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                  >  1:438726/1‑151 (MQ=255)                                                                                                               tttCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                                <  2:461162/149‑1 (MQ=255)                                                                                                               tttCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                              >  1:743942/1‑151 (MQ=255)                                                                                                                ttCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                             >  2:921496/1‑151 (MQ=255)                                                                                                                  cTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt                           >  1:843065/1‑151 (MQ=255)                                                                                                                   tACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                          >  2:1009677/1‑151 (MQ=255)                                                                                                                   tACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                          >  2:814909/1‑151 (MQ=255)                                                                                                                   tACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                          >  1:1209463/1‑151 (MQ=255)                                                                                                                    aCAGAC‑AAAAAAAAGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc                         <  1:1136954/151‑1 (MQ=255)                                                                                                                             aaaaaaGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt                 >  2:771498/1‑151 (MQ=255)                                                                                                                                aaaGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc               >  2:263296/1‑150 (MQ=255)                                                                                                                                 aaGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCaa             <  2:900950/151‑1 (MQ=255)                                                                                                                                  aGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAat            >  2:167487/1‑151 (MQ=255)                                                                                                                                  aGGCGACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAat            >  1:816321/1‑151 (MQ=255)                                                                                                                                      gACTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg        >  1:289579/1‑151 (MQ=255)                                                                                                                                       aCTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc       >  2:147055/1‑151 (MQ=255)                                                                                                                                       aCTCA‑TCAGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc       <  1:252585/151‑1 (MQ=255)                                                                                                                                           a‑tcaGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc   >  1:944795/1‑151 (MQ=255)                                                                                                                                             tcaGTAGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCg  >  1:476827/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCA‑TCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGG‑ATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCG  >  NC_000913/3815680‑3815968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑