Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 546,309 C→T A19T (GCT→ACT)  allD ← ureidoglycolate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913546,3090CT98.9% 340.4 / ‑4.5 88A19T (GCT→ACT) allDureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (42/45);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGCCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAAAA  >  NC_000913/546161‑546453
                                                                                                                                                    |                                                                                                                                                
cGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCt                                                                                                                                                <  1:983034/151‑1 (MQ=255)
cGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCt                                                                                                                                                <  1:454619/151‑1 (MQ=255)
 gAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTg                                                                                                                                               >  1:1174244/1‑151 (MQ=255)
    aCTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCa                                                                                                                                            <  2:45880/151‑1 (MQ=255)
      tCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGc                                                                                                                                             >  2:17685/1‑148 (MQ=255)
        cGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGt                                                                                                                                        <  2:896345/151‑1 (MQ=255)
            tCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTa                                                                                                                                     <  2:483329/150‑1 (MQ=255)
             cgcgGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTAtt                                                                                                                                   >  1:5394/1‑151 (MQ=255)
             cgcgGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTAtt                                                                                                                                   >  1:145737/1‑151 (MQ=255)
             cgcgGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTAtt                                                                                                                                   >  1:227943/1‑151 (MQ=255)
               cgGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATtct                                                                                                                                 <  1:378443/151‑1 (MQ=255)
                ggTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATtctc                                                                                                                                <  1:174067/151‑1 (MQ=255)
                 gTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCa                                                                                                                               <  2:1142618/151‑1 (MQ=255)
                   tGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAAt                                                                                                                             >  2:956886/1‑151 (MQ=255)
                    ggTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAAtt                                                                                                                            <  2:716665/151‑1 (MQ=255)
                       gccgccTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGc                                                                                                                         >  2:159150/1‑151 (MQ=255)
                         cgccTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCtg                                                                                                                       >  1:848742/1‑151 (MQ=255)
                         cgccTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCtg                                                                                                                       <  1:748705/151‑1 (MQ=255)
                             ttttGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCtggtgg                                                                                                                   >  1:813059/1‑151 (MQ=255)
                               ttGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCtggtg                                                                                                                    >  2:1197642/1‑148 (MQ=255)
                                tGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAg                                                                                                                 <  1:1144578/150‑1 (MQ=255)
                                tGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAg                                                                                                                 <  1:1133273/150‑1 (MQ=255)
                                 gAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgtg                                                                                                               <  2:262454/151‑1 (MQ=255)
                                 gAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgtg                                                                                                               <  1:967782/151‑1 (MQ=255)
                                 gAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgtg                                                                                                               >  2:756012/1‑151 (MQ=255)
                                 gAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgt                                                                                                                <  2:496197/150‑1 (MQ=255)
                                 gAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgt                                                                                                                <  1:712555/150‑1 (MQ=255)
                                  aaaTGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgtgt                                                                                                              >  1:294765/1‑151 (MQ=255)
                                  aaaTGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAgtgt                                                                                                              >  2:1199462/1‑151 (MQ=255)
                                   aaTGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGtt                                                                                                             >  1:217608/1‑151 (MQ=255)
                                      gCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTcc                                                                                                          >  2:442437/1‑151 (MQ=255)
                                      gCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTcc                                                                                                          >  1:46260/1‑151 (MQ=255)
                                       cGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTccc                                                                                                         >  1:149174/1‑151 (MQ=255)
                                       cGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTccc                                                                                                         >  1:761406/1‑151 (MQ=255)
                                         ttCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCg                                                                                                        >  1:356090/1‑150 (MQ=255)
                                          tCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGa                                                                                                       <  1:743514/150‑1 (MQ=255)
                                            cgcgTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTg                                                                                                    <  1:41705/151‑1 (MQ=255)
                                             gcgTAGTATTCCACGCGCTCCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGa                                                                                                   <  1:258963/151‑1 (MQ=255)
                                             gcgTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGa                                                                                                   <  2:1183575/151‑1 (MQ=255)
                                              cgTAGTATTCCACGCGCTCCGCGACATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTTCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGa                                                                                                       <  2:373593/146‑1 (MQ=255)
                                              cgTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGAt                                                                                                  >  2:347178/1‑151 (MQ=255)
                                                      tCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATc                                                                                           <  1:33446/150‑1 (MQ=255)
                                                        cACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGCCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGt                                                                                        <  2:553579/151‑1 (MQ=255)
                                                           gcgcACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCtt                                                                                     >  2:317193/1‑151 (MQ=255)
                                                            cgcACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTc                                                                                    >  1:588315/1‑151 (MQ=255)
                                                             gcACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTcc                                                                                   >  2:968600/1‑151 (MQ=255)
                                                              cACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCt                                                                                  <  1:615414/151‑1 (MQ=255)
                                                               aCCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCtt                                                                                 >  2:245290/1‑151 (MQ=255)
                                                                ccGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCttt                                                                                <  2:788728/151‑1 (MQ=255)
                                                                   cgcCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCttttt                                                                              >  2:232929/1‑150 (MQ=255)
                                                                     ccATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAac                                                                           <  2:615759/151‑1 (MQ=255)
                                                                     ccATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAac                                                                           >  1:1158148/1‑151 (MQ=255)
                                                                         gagaGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAacgacg                                                                       >  1:449284/1‑151 (MQ=255)
                                                                              tGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGtgtg                                                                  >  1:917012/1‑151 (MQ=255)
                                                                               ggATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGa                                                                 <  2:583036/151‑1 (MQ=255)
                                                                               ggATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGa                                                                 <  2:713149/151‑1 (MQ=255)
                                                                                gATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGaa                                                                <  2:1192934/151‑1 (MQ=255)
                                                                                 aTCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGa                                                                 >  1:83196/1‑149 (MQ=255)
                                                                                        tGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACt                                                        >  2:535017/1‑151 (MQ=255)
                                                                                         ggCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTg                                                       <  2:116886/151‑1 (MQ=255)
                                                                                         ggCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTg                                                       <  1:676455/151‑1 (MQ=255)
                                                                                            aTCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCa                                                     >  1:994886/1‑150 (MQ=255)
                                                                                              cGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAAtt                                                  <  2:1149677/151‑1 (MQ=255)
                                                                                                gCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTaa                                                <  1:1034224/151‑1 (MQ=255)
                                                                                                gCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTa                                                 >  2:511215/1‑150 (MQ=255)
                                                                                                       ccAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAg                                         <  2:149169/151‑1 (MQ=255)
                                                                                                            aCTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGaaa                                    <  2:1135349/151‑1 (MQ=255)
                                                                                                            aCTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGaa                                     >  1:1075032/1‑150 (MQ=255)
                                                                                                            aCTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGCGTGAAGCATGACTGCAATTAACATACAGGGaa                                     >  1:277797/1‑150 (MQ=255)
                                                                                                             cTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGaaa                                    >  2:473273/1‑150 (MQ=255)
                                                                                                               tCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAat                                  >  2:324669/1‑150 (MQ=255)
                                                                                                                 aGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATc                               <  1:163963/151‑1 (MQ=255)
                                                                                                                          tGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTAt                       <  1:17685/150‑1 (MQ=255)
                                                                                                                          tGCAGCGTGCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTAt                       <  2:510422/150‑1 (MQ=255)
                                                                                                                                  gCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATcc                >  1:646870/1‑149 (MQ=255)
                                                                                                                                  gCTCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCa               <  1:247222/150‑1 (MQ=255)
                                                                                                                                    tCACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGAc            >  2:1056185/1‑151 (MQ=255)
                                                                                                                                     cACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGa             <  1:1098845/149‑1 (MQ=255)
                                                                                                                                     cACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGAc            <  2:138346/150‑1 (MQ=255)
                                                                                                                                     cACGTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGAc            <  2:145732/150‑1 (MQ=255)
                                                                                                                                        gTTTTAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAgg         >  2:1206996/1‑150 (MQ=255)
                                                                                                                                         ttttAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGc        >  1:512952/1‑150 (MQ=255)
                                                                                                                                         ttttAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGc        >  1:112992/1‑150 (MQ=255)
                                                                                                                                          tttAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCa       <  1:538392/150‑1 (MQ=255)
                                                                                                                                           ttAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCaa      <  2:76990/150‑1 (MQ=255)
                                                                                                                                            tAACCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCaaa     <  2:350258/150‑1 (MQ=255)
                                                                                                                                             aaCCCAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCaaaaa   <  2:933439/151‑1 (MQ=255)
                                                                                                                                                ccAGTCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCaaaaaa  >  1:169755/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATACTTCAGCCACGGTTGCAGCGTGCTCACGTTTTAACCCAGCCTGGCAGAGTTTATTCTCAATTAGCTGGTGGAGTGTTTCCCGACTGATTTTCATCTGTCTTCCTTTTTAACGACGGTGTGAAGCATGACTGCAATTAACATACAGGGAAAATATCTGGATTATGTGATCCAGACAGGCAAAAAA  >  NC_000913/546161‑546453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: