Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA42.0% 62.5 / 27.7 50intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (25/4);  new base A (0/21);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 3.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTC  >  NC_000913/1824906‑1825199
                                                                                                                                                      |                                                                                                                                               
aCCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGAGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag                                                                                                                                                 <  1:529569/151‑1 (MQ=255)
    aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                              >  1:382404/1‑150 (MQ=255)
       ttttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaaga                                                                                                                                           >  1:418636/1‑150 (MQ=255)
           aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                      >  1:422689/1‑151 (MQ=255)
            cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                     >  1:140477/1‑151 (MQ=255)
            cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                     <  2:595168/151‑1 (MQ=255)
                    aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                              >  2:379072/1‑150 (MQ=255)
                    aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                             >  2:212356/1‑151 (MQ=255)
                    aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                             >  2:580775/1‑151 (MQ=255)
                            ccATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCg                                                                                                                     >  1:563278/1‑151 (MQ=255)
                                aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                 >  1:700721/1‑151 (MQ=255)
                                  tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                               >  2:676202/1‑151 (MQ=255)
                                     cTCCCGCCCCTTTTTTTTCTTTTTTATCGTTCAGTTACCGGGGGATGTTTATCGGCACCTGGGGTATTTTACTTTAGTGGTAATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                                <  1:378953/147‑1 (MQ=255)
                                      tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                            >  2:802811/1‑150 (MQ=255)
                                      tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGATGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                            >  2:28672/1‑150 (MQ=255)
                                       cccGGCCTGTTTTTTTCATTGTTATCGTGCGGTTACCGGGGGAGGTTTAGCGCCAACTCGGGTGTTTTACTTTGTTGGTGATCAGGTTTTTTTTAAAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa                                                                                                          <  1:784857/151‑1 (MQ=255)
                                       cccGGCCCTTTTTTTTCTTTTTTTTCTTTCAGGTAACGGTGGATGTTTATCGGCAACTGGGGTGATTTCCTTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                  <  1:793107/143‑1 (MQ=255)
                                       cccGGCCCTTTTTTTGCATTTTTCGCGTACGGGTACCGGTGGTTGGTTAGCGTAAGCTCGGTTATTTTACGTTGGGGGTGATCGGGTTTTTTTTGCACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc                                                                                                                      <  2:426191/139‑1 (MQ=255)
                                        ccGGCCGTTTCTTTTCTTGTTTTTCTTCCAGTTAACGGTGGAGGTTTATCGCAACCTGGGGTGATTTACTTTAGGGGTGACAAGTTTTTTTTTCCACCTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                            <  1:55843/148‑1 (MQ=255)
                                         cGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                        >  2:505258/1‑151 (MQ=255)
                                          gccccGTTTTTTTCATTTTTATCTTCCAGGTAACCGTGGATGGTTATCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                        <  1:839194/148‑1 (MQ=255)
                                             ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                    >  2:907006/1‑151 (MQ=255)
                                                   ttttCATTGTTATCTTTCAGGTACCCGTGGATGTTTATCGCCACCTCGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                    <  1:492014/145‑1 (MQ=255)
                                                     ttCATTTTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTGGGGTTATTTAGGTTAGGGGTGATCGGGTTTTTTTTACAATTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                             <  2:550843/150‑1 (MQ=255)
                                                       cATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                          >  1:376022/1‑151 (MQ=255)
                                                       cATTGTTATCGTACAGGTAACAGTGGCTGGTCACCGCCAACTCGGGTGATTTACGGTAGTGGCGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGATAGCCGCCGATCCAGACCACCGAATTACTGAAAAGTAGGGGACAAGAc                                                                                           >  2:43463/1‑150 (MQ=255)
                                                        aTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                         >  1:825598/1‑151 (MQ=255)
                                                         tttttATCGTTCAGTTACCCGTGGATGGTTATCGGCACCTCGGGTGTTTTACTTTAGTGGTGATCAGTTTTTTTTTACAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAc                                                                                           <  1:647351/145‑1 (MQ=255)
                                                              atgtttCGGGTACCCGTGGATGTTTATCGGCACCGCGGTTGATTTCCGTTAGGGGTGATAAGCTTTTTTTTACAATTGTTTGAAAGGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                     <  2:867248/145‑1 (MQ=255)
                                                              aTCGTCAAGGAAACCGTGGATGGTTACCGGAACCTGGGGTGATTTACGTTGGTGGTAATCAGGTTTTTTTTAAAATTGTTGGAGAAGAGAAAGAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                    <  1:202749/150‑1 (MQ=255)
                                                                  ttCAGGAACCCGTGGATGTTTCTCGGCAACGGGGGTGATTTATGTTAGTGTTGACCAGGTTTTTTTTACATTTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                 <  2:465843/149‑1 (MQ=255)
                                                                  tcagggtaccgggggATGTTTTCCGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACGGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                   <  1:482275/134‑1 (MQ=255)
                                                                     aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                            >  2:263463/1‑151 (MQ=255)
                                                                         aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                        >  2:291198/1‑151 (MQ=255)
                                                                             gttgttgtttATCGCCAACTCGGGTTATTTCCGTTAGTGGTGATCAGGTTTTTTTTCCAACTTTTGGAGAAGAAAAAAGAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg                                                                       <  1:142602/140‑1 (MQ=255)
                                                                                   gtttATCGGCACCTGGGGTTATTTACGTTAGTGGTGATCGGGTTTTTTTAACACCTGTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg                                                               <  1:953212/148‑1 (MQ=255)
                                                                                   gtttATCGGCACCTGGGGTGTTTTACTTTAGTGGTGATCAGGTTTTTTTTACACCTTTTTGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                <  2:274312/147‑1 (MQ=255)
                                                                                    ttttcgggCAACTGGGGTTATTTCCGTTAGTGGGGAAAGGGTTTTTTTTCCAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt                                                                    <  1:405979/138‑1 (MQ=255)
                                                                                      tgtcgccagcgggggTGTTTTACTTTGGTGGTGATCAGGTTTTTTTTACACTTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                              <  2:902955/136‑1 (MQ=255)
                                                                                        tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                         >  2:143398/1‑151 (MQ=255)
                                                                                                  gggTGATTTACGTTAGTGTTGATCGGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                  <  2:276264/148‑1 (MQ=255)
                                                                                                        tttACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                         >  1:298188/1‑151 (MQ=255)
                                                                                                                 gtggtgATCGGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                 <  2:230557/150‑1 (MQ=255)
                                                                                                                 gtggtgATCAGGTTTTTTTTACACCTGTTGAAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                       <  2:687455/144‑1 (MQ=255)
                                                                                                                 gtggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                >  2:261938/1‑151 (MQ=255)
                                                                                                                   ggtgATCAGGTTTTTTTTACAACTGTTGGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                <  1:523107/149‑1 (MQ=255)
                                                                                                                        tCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                            >  2:172106/1‑148 (MQ=255)
                                                                                                                         cgggttttttttAAATCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                         <  1:234447/145‑1 (MQ=255)
                                                                                                                               ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                  >  1:851939/1‑151 (MQ=255)
                                                                                                                                tttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                  >  1:918975/1‑150 (MQ=255)
                                                                                                                                  tttACAACTTTTTAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                <  2:124267/150‑1 (MQ=255)
                                                                                                                                     aCAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc             >  1:807556/1‑150 (MQ=255)
                                                                                                                                       aaCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc             >  1:867473/1‑148 (MQ=255)
                                                                                                                                                agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc  >  1:791641/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
ACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTC  >  NC_000913/1824906‑1825199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑