Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,434,882 | 0 | T | G | 41.4% | 98.8 / 13.2 | 58 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
Reads supporting (aligned to +/- strand): ref base T (11/23); new base G (24/0); total (35/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.99e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACC > NC_000913/2434735‑2435028 | ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAAt < 1:656153/151‑1 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCAcc > 2:897504/1‑150 (MQ=255) tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCAcc > 2:53697/1‑146 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCa < 2:284833/151‑1 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAATCCACcgcg > 1:828954/1‑147 (MQ=255) aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGa < 1:246042/150‑1 (MQ=255) ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAATCCACCCCGACCCGATa > 1:495251/1‑149 (MQ=255) cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCCAACCGGTCAGGGTAAATCCCCCCGACCAGaaa > 1:782060/1‑146 (MQ=255) ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGACATGTTCCGAGCATTATTCTCGTTAGCCCGCCAGAAATAGCGGGCCCATTGGCGGTCTTGGGAGAAAATAACGGCCAGGGTATTCCCTCGCGACCAGa > 1:646559/1‑145 (MQ=255) tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTcc > 2:918770/1‑151 (MQ=255) cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc < 1:35469/150‑1 (MQ=255) cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTc < 2:466977/149‑1 (MQ=255) tCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCCGGGGAATACACCGCGACCAGATAAAGCCCCTTCGc > 2:829576/1‑150 (MQ=255) cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCt < 2:116566/150‑1 (MQ=255) aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTGCCGtt > 1:758708/1‑151 (MQ=255) ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtg < 2:762581/151‑1 (MQ=255) cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGc < 1:207728/151‑1 (MQ=255) ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATCGTGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGAGTAATCCGCCGCGACCAGATACAGCCCTTCCGCTTTTGCCCTTTCTGCCGCCAGCGTTc < 2:439598/151‑1 (MQ=255) ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGtt < 1:372736/150‑1 (MQ=255) gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTAACCGGCAGGGGAATCCACCGCGACCAGATACAACCCCTCCCCTTTTTCCCGTGCTGCCCCCCGCGtt > 2:147638/1‑149 (MQ=255) aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGt < 1:90360/150‑1 (MQ=255) aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCCCCGCGACCAGAAACAGCCCCTCCGCTTTTTCCGTTGCTGCCCCCCGCGGTCTGTcc > 2:657257/1‑150 (MQ=255) aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCCCTTTTTCCGTTGCTGCCCCCAGCGGTCTGTcc > 1:296826/1‑150 (MQ=255) ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATTCCGGTCCAGGGAATCCCCCGCGGCCAGAAACAACCCCTTCCCCTTTGCCGGTTCTGCCGCCCGCGGTCTGTCCttt > 1:817545/1‑151 (MQ=255) cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGGCAGGGGAATCCACCGCGACCCGATACAACCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCGTTCTGTCCttt > 1:379186/1‑150 (MQ=255) aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGTCGTTGCTGCCCCCAGCGTTCTGTCCTTTtccgcc > 1:5522/1‑151 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAg < 1:38695/151‑1 (MQ=255) ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAg > 1:177632/1‑150 (MQ=255) aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGTAATCCACCGCGACCAGAAACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCaaacc > 1:267908/1‑148 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCa < 1:393692/149‑1 (MQ=255) ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGTTTTGTCCTTTTCCGCCAGCCACTCTGCTATCCAGctc > 1:539649/1‑151 (MQ=255) tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTTCGCCTTTTCCGGTGCTGCCGCCCGCGGTCTGTCCTTTGCCGCCAGCAACTCTGCTTTTCAGctc > 1:19851/1‑150 (MQ=255) aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct > 1:155768/1‑150 (MQ=255) aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCCGGGGAATCCCCCCCGACCCGAAACAACCCCTCCGCTTTTGCCGGTGCCGCCGCCCGCGGTTTGTGCCTTTCCGCCCGCAActcctctctcccacttt > 1:448398/1‑136 (MQ=255) cGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCGCGACCAGAAACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCAGCCACTCTGCTATTCAGCTCTCCgg > 2:302256/1‑150 (MQ=255) gCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTTCTGCCCCCAGCGTTCTGTCCTTTTCCGCCCGCAACCCTTCTATCCAGCTCTcc > 2:115603/1‑147 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGGGTTTGGAAGATCATACCGGTGAGGGTAAACCACCCCGACCAGATACAGCCCCTCCGCTTTTGCCCCTGGCGCCGCAAGCGTTCTGTCTTTTTCCGCCAGGCACTCTCCTATCCACATCTCCCGCTg > 2:141077/1‑151 (MQ=255) cAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCCGATACAGCCCTTCCGCTTTTTCCGTTTCTGCCCCCCGCGCTCTTTCCTTTGCCCCCAGCCACTCTGCTATCCAGCCCTCCGGCTgg > 2:249410/1‑151 (MQ=255) gAAATAGCGGGCCCATTGGCGGGTTTGGAAGATCATACCGGGCAGGGGAATCCACCGGGACCAGATACAGCCCTTCCGCTTTTTCCGTTGGTGCCGCCAGCGGTCTGTGCTTTTGCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgggt > 1:689307/1‑149 (MQ=255) aaaTAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCAGCCACTCTGCTATCCAGCTCTCCCGCTgggt > 2:938166/1‑148 (MQ=255) aaaTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtg < 1:936002/151‑1 (MQ=255) aTAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCCCCGCGACCAGATAAAGCCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCACCTCTCCGGCTGGGtgtg > 2:413807/1‑151 (MQ=255) aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg < 2:651415/151‑1 (MQ=255) aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg < 1:108388/150‑1 (MQ=255) gggCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc < 2:301056/151‑1 (MQ=255) cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGa < 1:885444/151‑1 (MQ=255) tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTc < 2:283003/150‑1 (MQ=255) ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc > 2:869301/1‑150 (MQ=255) gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg < 2:488620/151‑1 (MQ=255) ttttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg > 1:520468/1‑151 (MQ=255) ttttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg < 1:255437/150‑1 (MQ=255) ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGGCCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg < 2:660128/149‑1 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg > 2:305181/1‑151 (MQ=255) gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc > 2:634511/1‑151 (MQ=255) aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg < 2:850638/151‑1 (MQ=255) cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTTCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat > 1:925433/1‑151 (MQ=255) tCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg > 2:941855/1‑150 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc > 2:756489/1‑151 (MQ=255) | GGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACC > NC_000913/2434735‑2435028 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |