Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	44.0%	52.6 / 31.5	50	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (24/4);  new base A (0/22);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222446‑1222745                                                                                                                                                       |                                                                                                                                                      ggCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCg                                                                                                                                                       <  1:918241/151‑1 (MQ=255)   cAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGa                                                                                                                                                      >  1:47267/1‑150 (MQ=255)    aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaaa                                                                                                                                                    <  1:318847/151‑1 (MQ=255)    aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaa                                                                                                                                                     >  2:536727/1‑150 (MQ=255)        cTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaaca                                                                                                                                                 >  2:182375/1‑150 (MQ=255)         tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaa                                                                                                                                                >  2:131484/1‑150 (MQ=255)          ggCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaa                                                                                                                                               <  1:791522/150‑1 (MQ=255)           gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaac                                                                                                                                              >  2:11077/1‑150 (MQ=255)           gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaac                                                                                                                                              >  2:541682/1‑150 (MQ=255)             attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAgg                                                                                                                                           >  2:811683/1‑151 (MQ=255)               tacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGc                                                                                                                                          <  1:20117/150‑1 (MQ=255)                 cccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCg                                                                                                                                       >  1:487462/1‑151 (MQ=255)                                 tttCTGCACCCTGTGCGTTGTCATTTTTTGCCATTTCGTTGGTTTCTGCGCAAGTAATATAGGTTTCGGACGGCAAACCTTTTTTAATATTGTAGCCCGCCCCCCAGTTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAAt                                                                                                                         <  2:227349/149‑1 (MQ=255)                                 tttCTGCACCCTGTGCGCTGTCTTTTTTTGCCTTTTCTTTGTCTTCGCCGAAATAAATATAGTTTTCGGCCGGAAAACCTTTTTTAATATTGTACTCCGCCCCCAATGTCTTTGGGGAAAAAACAAACAGGCCCGCCAGTAAAGCaa                                                                                                                           <  2:824524/147‑1 (MQ=255)                                 tttCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAttt                                                                                                                       >  2:5461/1‑151 (MQ=255)                                    cTGCACCCTGTGCGCTGTCATTTTTTGCCTTTTCGTTGTCTTCTGCGAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTAAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAAtttt                                                                                                                      <  1:906355/149‑1 (MQ=255)                                       cAACCTGTGCGCTGTCAGTTTTTGCCTTTTCTTTGTCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATTTTGTAGCCCTGCCCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcat                                                                                                                 <  1:700729/151‑1 (MQ=255)                                       cAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcat                                                                                                                 >  2:837026/1‑151 (MQ=255)                                         aCCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                                >  2:10436/1‑150 (MQ=255)                                              ggcgtTGTCATTTTTTGCATTTTCTTTGGCTTCTGCGAAAGAAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCGGCGCCCAGTTTTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                          <  2:710115/150‑1 (MQ=255)                                               gcgcTTTCAGTTTTTGCCTTTTCGTGGGCTTTGGCGCAAGTAATATAGTTTTCGGCCGGCAAACCGTTTTTAATTTTGTAGCCCTGCGCCCGGGGTATTGGGGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                          <  1:491172/150‑1 (MQ=255)                                                 gttttctttttttCCCTTTTCGTGGTTTTCTGCCAAATTAAATTAGTTTTCTGACGGAAAACCTTTTTTAATTTTGTAGCCCGCCCCCCAGTTTATTGGGCCAAAAACAAAAAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTc                                                                                                         <  2:950933/141‑1 (MQ=255)                                                         tttttGCCTTTTGGTTGTTTTCGGGGCAAGTAATATGGTTTTCTGACGGCAACCTTTTTTAAATATTGTGGCCGCGCCCCCGGTTCTTGGGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  <  2:701365/148‑1 (MQ=255)                                                               ccttttCGTTGGCTTCTGCGAAAGTATTAGTGGTTCCTGACGGAAACCCTTTTTTAATTTGGTGTCCCGGCGCCCAGGTCTTGGTTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTa                                                                                           <  1:246799/146‑1 (MQ=255)                                                                     cGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   >  1:441866/1‑151 (MQ=255)                                                                      gTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                                  >  1:764654/1‑151 (MQ=255)                                                                       ttGGTTTCGGCGCAAGTAATATGGGTTTCGGACGGCAACCCTTTTTAAATATTGTAGCCCTCCCCCCAGTTTTTTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   <  2:774988/149‑1 (MQ=255)                                                                       ttGGCTTCTGCGCAAGTAATATAGTTTTCTGACGGAAAACCTTTTTAAATATTGTAGCCCTGCGCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   <  1:918652/149‑1 (MQ=255)                                                                             tCTGCGCAATTAATTTAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCGGCGCCCAGGTATTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacca                                                                            <  1:179579/150‑1 (MQ=255)                                                                             tCTGCAAAATTAATATAGTTTTCTGACGCCAAACCTTTTTAAATATTGTAGCCTGGCCCCCAGTTTTTTGGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGca                                                                               <  2:43664/147‑1 (MQ=255)                                                                                 cacAAGTAATAGGGGTTTCTGCCGGCAACCCTTTTTTAATTTTGTAGCCCTGCGCCCAGGTCTTTGGTGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCAccc                                                                         <  2:371920/147‑1 (MQ=255)                                                                                  gcAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGt                                                                       >  2:56350/1‑150 (MQ=255)                                                                                    aaGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAcc                                                                    >  2:596822/1‑151 (MQ=255)                                                                                              ggTTTCTGACGGCAAACGGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCGCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                          <  1:764674/151‑1 (MQ=255)                                                                                              ggTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                          >  1:521604/1‑151 (MQ=255)                                                                                                   ccgACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCAcc                                                     >  2:621973/3‑151 (MQ=255)                                                                                                             aCCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcc                                           >  1:763441/1‑151 (MQ=255)                                                                                                                 tttttAATTTTGTCGCCCGGCGCCAAGGTCATTGGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcccc                                         <  1:516140/149‑1 (MQ=255)                                                                                                                    ttAATTTTGTAGTCCTGCCCCCAGTTCATTGGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                                     <  1:603253/150‑1 (MQ=255)                                                                                                                      aaTTTTGTAGCCCTCCCCCCAGGTCTTTGGTGCAAAAACAAACGCCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAg                                   <  2:69199/150‑1 (MQ=255)                                                                                                                      aaTATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAgg                                  >  2:89153/1‑151 (MQ=255)                                                                                                                         atTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGcc                                >  1:592735/1‑150 (MQ=255)                                                                                                                                 ccggcgcCCAGTTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCgg                        <  1:673211/147‑1 (MQ=255)                                                                                                                                 ccggccccaggtttaTTGGTGAAAAAAAAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCg                         <  2:623422/135‑1 (MQ=255)                                                                                                                                 ccTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGt                       >  1:428502/1‑151 (MQ=255)                                                                                                                                    gcgcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTc                      >  1:497011/1‑149 (MQ=255)                                                                                                                                      gcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   >  2:919828/1‑150 (MQ=255)                                                                                                                                       cctagttttttGGTGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAAc                 <  1:11077/142‑1 (MQ=255)                                                                                                                                          aGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc              >  1:426537/1‑151 (MQ=255)                                                                                                                                           ggTCATGGGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc              <  1:697389/150‑1 (MQ=255)                                                                                                                                                    tGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACa       <  1:598364/148‑1 (MQ=255)                                                                                                                                                       gAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCAcc  >  2:629439/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                      GGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222446‑1222745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑