Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	362,830	C→A	C117F (TGT→TTT)	lacY ←	lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	362,830	0	C	A	100.0%	245.7 / NA	62	C117F (TGT→TTT)	lacY	lactose permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/41);  total (21/41)

GATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTT  >  NC_000913/362688‑362975                                                                                                                                                |                                                                                                                                                  gATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGcc                                                                                                                                            <  2:182704/150‑1 (MQ=255) gATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCt                                                                                                                                           >  2:496626/1‑151 (MQ=255)    ggTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGTCTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGa                                                                                                                                        <  2:34573/151‑1 (MQ=255)        aaCATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATaa                                                                                                                                     >  1:875000/1‑150 (MQ=255)             gatgCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATacca                                                                                                                               <  1:205073/151‑1 (MQ=255)                    aCAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACcaaca                                                                                                                         >  2:695967/1‑150 (MQ=255)                     cAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAAt                                                                                                                       <  1:290063/151‑1 (MQ=255)                       aTCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCg                                                                                                                     <  2:950623/151‑1 (MQ=255)                             gCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCcta                                                                                                               <  1:944579/151‑1 (MQ=255)                              cacacaGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCctac                                                                                                              <  2:208659/151‑1 (MQ=255)                                         cAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtgttgt                                                                                                   <  2:141293/151‑1 (MQ=255)                                           gCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtat                                                                                                 <  2:939685/151‑1 (MQ=255)                                           gCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtat                                                                                                 <  1:290036/151‑1 (MQ=255)                                            ccAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtatt                                                                                                >  1:10894/1‑151 (MQ=255)                                             cAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtattg                                                                                               <  1:2168/151‑1 (MQ=255)                                                cacaGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtattgta                                                                                             >  2:461695/1‑150 (MQ=255)                                                    gCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGt                                                                                        >  2:225874/1‑151 (MQ=255)                                                    gCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCGACTAAAATGTTGTATTGTAACAGt                                                                                        >  1:567139/1‑151 (MQ=255)                                                     ccAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGt                                                                                        <  2:44990/150‑1 (MQ=255)                                                      cAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTgg                                                                                      >  2:272411/1‑151 (MQ=255)                                                        aaCATTCCCGCGCGGCCAAAATTGGAATTACCGCTACCGATTACGTTCTCAATTAATGGCTTTTCCTCCTGCCCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTg                                                                                       <  2:694858/148‑1 (MQ=255)                                                           aTCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGa                                                                                 <  1:952775/151‑1 (MQ=255)                                                            tCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGa                                                                                 >  2:882741/1‑150 (MQ=255)                                                             ccGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAg                                                                               <  1:951595/151‑1 (MQ=255)                                                              cgcgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGa                                                                              <  2:27665/151‑1 (MQ=255)                                                              cgcgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGa                                                                              <  2:431386/151‑1 (MQ=255)                                                                cgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATa                                                                            <  2:771330/151‑1 (MQ=255)                                                                 gcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATa                                                                            <  2:425914/150‑1 (MQ=255)                                                                    cgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaaaaa                                                                        <  2:223106/151‑1 (MQ=255)                                                                         aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATaaa                                                                    >  2:33942/1‑150 (MQ=255)                                                                         aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATaa                                                                     >  2:548246/1‑149 (MQ=255)                                                                         aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAg                                                                   <  1:462763/151‑1 (MQ=255)                                                                             tCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAAc                                                                <  1:829257/150‑1 (MQ=255)                                                                             tCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACg                                                               >  1:568801/1‑151 (MQ=255)                                                                              cGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACgg                                                              <  2:482578/151‑1 (MQ=255)                                                                              cGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACg                                                               <  2:640829/150‑1 (MQ=255)                                                                                    tACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCaa                                                         >  1:656939/1‑150 (MQ=255)                                                                                     aCTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAAc                                                       <  2:219980/151‑1 (MQ=255)                                                                                       tGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCTCCAACAATCGATCCTCCAAAAATGTTGTATTGTAACAGCGGCCCGAAGATAAAAATAAAGAACGgcg                                                            <  2:342714/144‑1 (MQ=255)                                                                                        gCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATc                                                    <  2:771994/151‑1 (MQ=255)                                                                                         cGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCa                                                   <  1:734261/151‑1 (MQ=255)                                                                                           aCGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACt                                                 <  2:546122/151‑1 (MQ=255)                                                                                            cGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACt                                                 <  2:646090/150‑1 (MQ=255)                                                                                                 gACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACAt                                            <  1:652842/150‑1 (MQ=255)                                                                                                     ttCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCg                                        <  2:924744/150‑1 (MQ=255)                                                                                                      tctcAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCgg                                       <  1:863518/150‑1 (MQ=255)                                                                                                          aaTAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGtaat                                   >  1:457199/1‑150 (MQ=255)                                                                                                             aaaTGCCTCTACTGCTGGCGCACCGGCGTTAAAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGtaataa                                 <  1:87915/150‑1 (MQ=255)                                                                                                                 gCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcaca                           <  1:342155/151‑1 (MQ=255)                                                                                                                 gCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcac                            <  1:439863/150‑1 (MQ=255)                                                                                                                 gCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCcac                            >  2:50694/1‑150 (MQ=255)                                                                                                                   ctctACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCAcagc                         >  2:418175/1‑151 (MQ=255)                                                                                                                       aCTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGt                     <  2:100079/151‑1 (MQ=255)                                                                                                                        ctgctgGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGAGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGt                     >  2:285879/1‑150 (MQ=255)                                                                                                                          gctgGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTAtt                  >  1:292195/1‑151 (MQ=255)                                                                                                                              gcgcACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcg              <  1:328802/151‑1 (MQ=255)                                                                                                                              gcgcACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcg              >  2:273302/1‑151 (MQ=255)                                                                                                                               cgcACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcgc             <  1:300947/151‑1 (MQ=255)                                                                                                                                       cGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGa      <  2:534979/150‑1 (MQ=255)                                                                                                                                       cGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGa      <  1:570331/150‑1 (MQ=255)                                                                                                                                         tt‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAg     >  1:203102/1‑149 (MQ=255)                                                                                                                                          t‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGttt  >  1:238868/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  GATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTT  >  NC_000913/362688‑362975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑