Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,613	G→A	S45L (TCA→TTA)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,613	0	G	A	100.0%	210.8 / NA	55	S45L (TCA→TTA)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/31);  total (24/31)

TTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCC  >  NC_000913/2725466‑2725760                                                                                                                                                    |                                                                                                                                                    tttttGCGACCATGTTAATCGGCAATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTaaa                                                                                                                                                   <  1:608493/150‑1 (MQ=255)   tttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAAc                                                                                                                                                  >  2:210330/1‑149 (MQ=255)       cGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                            <  1:929550/151‑1 (MQ=255)                ttATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACa                                                                                                                                    <  1:597530/150‑1 (MQ=255)                ttATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACAt                                                                                                                                   >  1:423656/1‑151 (MQ=255)                    cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                                <  2:124135/150‑1 (MQ=255)                      gCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAAt                                                                                                                             <  1:564942/151‑1 (MQ=255)                          tGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGa                                                                                                                          >  2:580445/1‑150 (MQ=255)                           gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAc                                                                                                                        >  2:618964/1‑151 (MQ=255)                             ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCa                                                                                                                      <  1:488787/151‑1 (MQ=255)                              gCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCAc                                                                                                                     <  1:41459/151‑1 (MQ=255)                                 aaaTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCg                                                                                                                  >  2:13627/1‑151 (MQ=255)                                   aTAGCCACCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                                                                                                                <  2:714792/151‑1 (MQ=255)                                     aGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAcc                                                                                                               <  1:655383/150‑1 (MQ=255)                                         aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGAAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAtt                                                                                                          >  1:236028/1‑151 (MQ=255)                                              ccTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg                                                                                                     >  2:677693/1‑151 (MQ=255)                                               cTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg                                                                                                     >  2:954865/1‑150 (MQ=255)                                               cTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg                                                                                                     >  1:863760/1‑150 (MQ=255)                                                tATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGa                                                                                                    >  2:934640/1‑150 (MQ=255)                                                  ttGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAag                                                                                                  <  1:520690/150‑1 (MQ=255)                                                        gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGcccc                                                                                           >  1:905046/1‑151 (MQ=255)                                                            cAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAGATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCcac                                                                                        <  2:811232/150‑1 (MQ=255)                                                            cAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCcac                                                                                        <  1:152158/150‑1 (MQ=255)                                                              ggAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                     <  2:606193/151‑1 (MQ=255)                                                               gAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCATTCGACCAGATTACCTGAAGAGGCCCCCACAATc                                                                                    <  1:75825/151‑1 (MQ=255)                                                                 aTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGc                                                                                  <  1:777569/151‑1 (MQ=255)                                                                     cGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa                                                                              <  2:369932/151‑1 (MQ=255)                                                                      gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa                                                                              >  1:827297/1‑150 (MQ=255)                                                                        agcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATg                                                                           >  2:66046/1‑151 (MQ=255)                                                                               acacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgac                                                                    >  1:357513/1‑151 (MQ=255)                                                                                  ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAg                                                                 <  2:374715/151‑1 (MQ=255)                                                                                     gctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTa                                                               <  1:541323/150‑1 (MQ=255)                                                                                         tttGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCact                                                          >  1:925463/1‑151 (MQ=255)                                                                                            gtagtaGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                        <  1:490056/150‑1 (MQ=255)                                                                                                   ttGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGt                                                >  2:928581/1‑151 (MQ=255)                                                                                                    tGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGtt                                               >  1:750351/1‑151 (MQ=255)                                                                                                     gAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAAAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGttt                                              <  2:113738/151‑1 (MQ=255)                                                                                                       gtcgtcGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGc                                            <  2:418863/151‑1 (MQ=255)                                                                                                        tcgtcgTGTTCCCTGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTg                                             <  1:134339/149‑1 (MQ=255)                                                                                                         cgtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTg                                          >  2:546353/1‑151 (MQ=255)                                                                                                            cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                       >  2:330718/1‑151 (MQ=255)                                                                                                             gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTg                                      <  1:738801/151‑1 (MQ=255)                                                                                                               gtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGcc                                    >  1:590431/1‑151 (MQ=255)                                                                                                                  cccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                 <  2:333616/151‑1 (MQ=255)                                                                                                                  cccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                 >  2:642936/1‑151 (MQ=255)                                                                                                                          ggaagAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACt                         <  1:72924/151‑1 (MQ=255)                                                                                                                            aagaagATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt                       <  2:90985/151‑1 (MQ=255)                                                                                                                             agaagaTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt                       >  2:173584/1‑150 (MQ=255)                                                                                                                                agaTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgc                   >  2:606313/1‑151 (MQ=255)                                                                                                                                    gtgGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccat                <  2:285736/150‑1 (MQ=255)                                                                                                                                        gCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat           <  1:160609/151‑1 (MQ=255)                                                                                                                                        gCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat           <  2:756821/151‑1 (MQ=255)                                                                                                                                          aaaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCCCAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTa         <  2:105988/151‑1 (MQ=255)                                                                                                                                             gTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGt      <  1:673111/151‑1 (MQ=255)                                                                                                                                               agagTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtct    >  1:348170/1‑151 (MQ=255)                                                                                                                                                 agTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc  <  1:680394/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCC  >  NC_000913/2725466‑2725760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑