Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC52.6% 74.6 / 103.8 57T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (25/2);  new base C (0/30);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 3.53e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-11

GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGT  >  NC_000913/1376356‑1376644
                                                                                                                                                          |                                                                                                                                           
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac                                                                                                                                            >  2:122233/1‑151 (MQ=255)
     ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCg                                                                                                                                       <  1:757300/151‑1 (MQ=255)
          gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                  >  1:131623/1‑151 (MQ=255)
             gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGcc                                                                                                                               >  1:830331/1‑151 (MQ=255)
               ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccg                                                                                                                             >  1:1049829/1‑151 (MQ=255)
               ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccg                                                                                                                             <  2:1122148/151‑1 (MQ=255)
               ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccg                                                                                                                             >  1:378107/1‑151 (MQ=255)
                            cgtGGACGGCGACCGAAAAATGGTGGGGTATCCCCGGCGCGTACTGAACGGGGCGGCCGCGCGTCCCGGAACGGTGGTGTATCTCAACCCGTTAACCCGCTATAAGGTCCATATAACCAACCCCCTCGCCGATGCCCGCCGTTa                                                                                                                       <  2:517772/142‑1 (MQ=255)
                               ggccGGCAACCGAAAAATGGGGGGTGATCCCCTGCGCGTACTGAAACGGGTCCCCGCGCTTCCCGGACCCGTGGTGTATCTCGAACCGTAAACCCGCTATCAGGATAATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGaa                                                                                                             <  1:325583/148‑1 (MQ=255)
                                   ggCGCCCGCAAAAGGTGGAGTGTTCCCCTGCGCGTACTGAAACGGGTCGCCGCGCGTCCCGGACCGGTGGTTTATCCCGACCCTTTAACCCCTTATCAGGTTCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGaaa                                                                                                            <  1:664866/148‑1 (MQ=255)
                                   ggCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                         >  2:380539/1‑151 (MQ=255)
                                       aCCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                     >  2:150111/1‑151 (MQ=255)
                                        ccgaaaaggggtgggtgTTCCCCTGCGCTTACGGGACCGGGCCGCCGCGGGTCCGGGACCGGGGGTGTATCTCGACCCTTAAACCCGCTATCAGGATAATATAATCAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                       <  2:516839/139‑1 (MQ=255)
                                        ccGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                    >  2:962762/1‑151 (MQ=255)
                                         cGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                    >  1:760801/1‑150 (MQ=255)
                                               aTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                             >  1:14974/1‑151 (MQ=255)
                                                 ggTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTCCCGAACCGGTGTTGTATCTCGACCGGTAAACCCGCTACCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                             <  1:1130433/149‑1 (MQ=255)
                                                 ggTGAGTGATCCCCTGCGCTTACTGGAACAGGTCGCCGCGCGTCCCGGACCCGGGGTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                             <  1:255488/149‑1 (MQ=255)
                                                    gagtgaTTCCCTGCGCGTACGGGAACAGGTCGCCGCGCGTCCGGAACCCGTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                          <  1:1184414/149‑1 (MQ=255)
                                                      gtgaTTCCCGGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGACCCGTGGTGTATCTCAACCCGTAAACCCGCTATAAGAATAATATGAACAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                      <  1:1100953/151‑1 (MQ=255)
                                                         aTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                   >  1:682864/1‑151 (MQ=255)
                                                            cccTGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGAACCCGTGGTGTACCTCGACCCGTAAACCCGTTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                  <  1:725951/149‑1 (MQ=255)
                                                            cccGGCGCGTACTGGAACAGGTCCCCGCGCGTCCCGGAACCGTGGTGTATCTCGAACCTTTAACCCGCTATCAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                     <  1:382368/146‑1 (MQ=255)
                                                                  gcgTACTGAAACGGGTCCCCGCGCGTCCGGGAACCGGGGTGTATCTCAACCCTTTAACCCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                           <  1:1192339/150‑1 (MQ=255)
                                                                    gTACTGGAACAGGTCGCCGCGCGTACGGGACCGGTGGTGTATCTCGACCTTTAAACCCTCTATCAGGATCATATAATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTc                                                                         <  2:527689/150‑1 (MQ=255)
                                                                     tacggaaaCGGGTCCCCGCGCGTCCCGGACCGGTGGTGTATCCCGACCCTTAAACCCGCTATCAGAATAATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGa                                                                             <  2:1021041/139‑1 (MQ=255)
                                                                     tACTGGAACGGGTCGCCGGGCGTCCCGGACCCGGGTTGTATCCCGAACCTTAAACCCGTTAACAGGATCATATGAACACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                        <  2:334376/150‑1 (MQ=255)
                                                                        tGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                     >  1:803511/1‑150 (MQ=255)
                                                                        ggaaacggtgcgccgcGCGTACCGGAACGGGGGTGTATCCCGAACCTTAAACCCCTTATCAGTATAATAGAATCAACCCCCTCGCCTTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                       <  2:1034406/138‑1 (MQ=255)
                                                                              aGGTCGCCGCGGGTCCGGAACCGGGGGTGTATCTCGACCGGTAAACCCGCTATCTGGATTATATGATCAACCCCCCCGCAGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcaca                                                                  <  1:539729/147‑1 (MQ=255)
                                                                              aGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                               >  2:313162/1‑150 (MQ=255)
                                                                                     ccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                         >  2:379444/1‑150 (MQ=255)
                                                                                     ccgcGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                         >  2:955535/1‑150 (MQ=255)
                                                                                      cgcgcgTCCCGAACCGGTGGTTTCTCCCGACCCTTAAACCCGCTATCAGGATCATATAAACACCCCCCTCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                        <  2:559577/150‑1 (MQ=255)
                                                                                        cgcgTCCCGGACCCGTGTTGTTCCACAACCCTTTAAACCTCTATCAGGATCATATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                      <  2:648424/150‑1 (MQ=255)
                                                                                         gcgccccGGACCGGTGGTGTTCCCCGCCCCTTAAACCCGTTATCAGAATAATATAACCACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                      <  2:57167/144‑1 (MQ=255)
                                                                                            tcccGGACCCGGTGTGTATCTCGACCCTTAAACCCGTTATCGGGATCATATGACAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaaga                                                  <  1:305346/148‑1 (MQ=255)
                                                                                             gtaccaaaccggtggtgTATCAATAACGGTAAAGCCTATACCAGAATTATAAAATCATCCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                        <  2:961666/134‑1 (MQ=255)
                                                                                                ggAACGGTGGTGTATCTCGAACCGTAAACCCGCTATCAGGATCATATGAACAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                 <  1:181755/147‑1 (MQ=255)
                                                                                                      cggtgtttttcctcGACCCGTAAACCCGCTATCAGGATCATATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                              <  2:496795/135‑1 (MQ=255)
                                                                                                      cggtggtgTTTCTCAACCCGTAAACCCGCTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                           <  1:222722/147‑1 (MQ=255)
                                                                                                          ggtgTATCTCGAACCGTAAACCCGCTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCa                                      <  1:530141/150‑1 (MQ=255)
                                                                                                           ttgtACCTCGACCCTTTAACCCGTTATAAGAATCATATAACAAACCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                           <  2:50649/143‑1 (MQ=255)
                                                                                                            gttttcccGACCCGTAAACCCGCAATCAGGATAATATGAACAACCCCCGCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                   <  2:619648/144‑1 (MQ=255)
                                                                                                                tctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                               >  2:427410/1‑151 (MQ=255)
                                                                                                                       cgtttAACCCGTTATCAGGATAATATGACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                               <  1:482267/141‑1 (MQ=255)
                                                                                                                       ccGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                         >  2:1201032/1‑150 (MQ=255)
                                                                                                                          ttAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc                     >  1:628880/1‑151 (MQ=255)
                                                                                                                           tAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                    >  2:1006399/1‑151 (MQ=255)
                                                                                                                            aaaCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                   >  1:704339/1‑151 (MQ=255)
                                                                                                                             aacccccTATCAGGATAATATGACCACCCCCCTCGCGTATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                   <  2:647375/145‑1 (MQ=255)
                                                                                                                              aCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                  >  1:1107990/1‑150 (MQ=255)
                                                                                                                                        cAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg         >  2:338614/1‑151 (MQ=255)
                                                                                                                                         aGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCgg        >  1:112313/1‑151 (MQ=255)
                                                                                                                                         aGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCgg        >  1:247651/1‑151 (MQ=255)
                                                                                                                                          ggATTATAAGAACACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGt       <  2:871648/151‑1 (MQ=255)
                                                                                                                                            aTCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTc      >  2:1060224/1‑150 (MQ=255)
                                                                                                                                               atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt  >  1:741303/1‑151 (MQ=255)
                                                                                                                                                          |                                                                                                                                           
GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGT  >  NC_000913/1376356‑1376644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: