Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA51.2% 91.9 / 162.9 86intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (30/12);  new base A (0/44);  total (30/56)
Fisher's exact test for biased strand distribution p-value = 8.61e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-16

TTTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29336‑29633
                                                                                                                                                          |                                                                                                                                                   
tttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcac                                                                                                                                                     >  2:790035/1‑151 (MQ=255)
tttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcac                                                                                                                                                     >  1:255310/1‑151 (MQ=255)
tttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcac                                                                                                                                                     >  1:14924/1‑151 (MQ=255)
 ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGTATGAGCcac                                                                                                                                                     >  1:1022445/1‑150 (MQ=255)
  tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcaca                                                                                                                                                    <  1:400726/150‑1 (MQ=255)
     gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaat                                                                                                                                                <  2:465002/151‑1 (MQ=255)
             aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                         <  2:1151852/150‑1 (MQ=255)
             aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                         <  1:631527/150‑1 (MQ=255)
              aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                       <  2:842026/151‑1 (MQ=255)
                 tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                     <  2:168338/150‑1 (MQ=255)
                  aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    <  1:284140/150‑1 (MQ=255)
                  aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATc                                                                                                                                   <  2:832907/151‑1 (MQ=255)
                   aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                  <  2:675465/151‑1 (MQ=255)
                      tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                <  1:964078/150‑1 (MQ=255)
                        tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                              <  2:19129/150‑1 (MQ=255)
                                tGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                     >  1:623587/1‑151 (MQ=255)
                                 ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                     >  1:300923/1‑150 (MQ=255)
                                              cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                        >  1:869241/1‑150 (MQ=255)
                                                   cGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                  >  2:394919/1‑151 (MQ=255)
                                                    ttttttATTTCATGCAATCTTTTTGCTGCGCAAGCTTTTCCAAAAACGGTTTAGATGATTTTTTTGTCGTTTAATGCTTGAAAAAAATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGcc                                                                                                         <  1:241977/142‑1 (MQ=255)
                                                     ttttttttTCATGCATTCTTCTTGCTGCGAAAGCTTTTCCAAAAAAAGTTAAGATAATTTTTTTGTCTTTAATTGCTTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                 <  1:457473/150‑1 (MQ=255)
                                                     tttttATTTCATTCATTTTTCTTGCTGCGCAAGCGTTTTCCAAAACAGGTAAGATTATTTTTTTGTCTCTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCata                                                                                                     <  2:353239/146‑1 (MQ=255)
                                                     tttttATTTCATGCATTCTTCTTGCTGCGCAAGCTTTTTCAAGAACGGTTTAGATGATTTTTTTGCCGCTTAATGCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                    <  1:569715/147‑1 (MQ=255)
                                                     tttttATTTCATGCAATCTTCTTGCTGCGAAAGCTTTTTCAAAAAAAGGTTAGATGATTTTTTTGTCGCTAAAGGCCTGAAAAACATGCTTGGCCCACAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                 <  2:371466/150‑1 (MQ=255)
                                                     tttttATTTCATGAATTCTTCTTGCTGCGAAAGCGTTTTCAAGAACAGTTTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                    <  1:443476/147‑1 (MQ=255)
                                                      ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                >  1:1153747/1‑150 (MQ=255)
                                                         tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                             >  2:1138008/1‑150 (MQ=255)
                                                            ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                         >  2:378050/1‑151 (MQ=255)
                                                                        tcttGTTGCGCAAGCGTTTCCCAAAACAGTTTAGATGATTTTTTTGTCGTTTATTCCCTTAAAAACATGCATGAGCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                <  1:959123/148‑1 (MQ=255)
                                                                         cttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                              >  2:436835/1‑149 (MQ=255)
                                                                         cttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                              >  2:889360/1‑149 (MQ=255)
                                                                          ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                              >  2:734818/1‑148 (MQ=255)
                                                                           ttccggcgaaggcttttCCAAGAAGAGTTTAGATGATTTTTTTGTCGCTTAATCCTTGAAAAACTTCCATGCCCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                 <  2:936741/132‑1 (MQ=255)
                                                                           tgctgcGCAGTCTTTTTCAAGAACGGGTTAGATGATTTTTTTGTCGTTAAATCCCTAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  2:608612/150‑1 (MQ=255)
                                                                               gcgcAAGCTTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGTTTAATGCCTGTAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                      <  1:228028/151‑1 (MQ=255)
                                                                                cgaaaGCTTTTCCAAAAACAGGTTAGATGTTTTTTTTGCCGTTTAATGCCTGAAAACCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                         <  1:113735/144‑1 (MQ=255)
                                                                                cccAAGCTTTTTCCAAAAGATTTTAGATTATTTTTTTGTCGTTTAATGCCTGAAAAACATGCTTGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                        <  2:604008/146‑1 (MQ=255)
                                                                                   aagttttttCAAGAACAGGTTAGATGTTTTTTTTGTCGTTTAATGCTTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  1:906627/137‑1 (MQ=255)
                                                                                   aaGCTTTTTCCAAAACAGTTAAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                             <  1:53818/140‑1 (MQ=255)
                                                                                   aaGCTTTTTCAAGAACGGTTAAGATATTTTTTTTGTCCTTTAATGCTTGAAAAACATGAATGCGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  2:762243/142‑1 (MQ=255)
                                                                                         tttccaaaaaaaGGTTAAATATTTTTTTTGTCTTTTATTCCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               <  2:223533/138‑1 (MQ=255)
                                                                                         tttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  1:128783/1‑151 (MQ=255)
                                                                                         tttCCAGAACAGGTTAATTGATTTTTTTTTCCCTTAATGCTTGAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                             <  1:272761/150‑1 (MQ=255)
                                                                                          tcccAGAACGGTTTAGATTATTTTTTTGTCTCTTAACGCCTGAAAAACATGCGTGACCAAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            <  2:71119/148‑1 (MQ=255)
                                                                                           tCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  1:228358/1‑149 (MQ=255)
                                                                                            ccAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  2:199716/1‑148 (MQ=255)
                                                                                               aGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                       >  2:200429/1‑151 (MQ=255)
                                                                                                 accgggTTAGATGATTTTTTTGGCCCTAAAGGCTTCAAAAAATTGCATGACCCAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                     <  1:494616/147‑1 (MQ=255)
                                                                                                 aaCAGGTTAGATAATTTTTTTGTCGTTTAATGCCGGTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                      <  2:598672/150‑1 (MQ=255)
                                                                                                     ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                 >  1:987893/1‑151 (MQ=255)
                                                                                                      gTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                >  1:946441/1‑151 (MQ=255)
                                                                                                       ttAAATGTTTTTTTTGTCGTTAAAGCCTTAAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  1:751218/150‑1 (MQ=255)
                                                                                                        taagaatattttttttTCGTTAAATGCCTGAAAAACAGGCATAGGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                 <  1:602416/139‑1 (MQ=255)
                                                                                                              gatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTCTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                         >  1:813457/1‑151 (MQ=255)
                                                                                                              catCTTTTTGTCGCTTAATGCCTAAAAAACATGCATGCCCAAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                             <  2:617235/146‑1 (MQ=255)
                                                                                                                tttttttGTCGTTTAATGCCTTTAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                         <  2:513832/147‑1 (MQ=255)
                                                                                                                 cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       >  1:374854/1‑150 (MQ=255)
                                                                                                                 cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       >  2:956562/1‑150 (MQ=255)
                                                                                                                  tttttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                     >  1:110185/1‑151 (MQ=255)
                                                                                                                  tttttGTCGCTAATTGCCTTTAAAACATGCATGGCCAAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  2:305105/148‑1 (MQ=255)
                                                                                                                  tttttGTCGTTAAAGCCTAAAAAAACATAAAGGAACCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                          <  2:693513/146‑1 (MQ=255)
                                                                                                                   tttgcccactaaaTGCCTGAAAACCATGCACGGCCCAAAAAATAAAATAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                      <  1:1175005/138‑1 (MQ=255)
                                                                                                                      tGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 >  1:288466/1‑151 (MQ=255)
                                                                                                                        tCGCTTAATGCCTATAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                <  1:520109/150‑1 (MQ=255)
                                                                                                                         cctttAATGCCTAAAAAACATGAATGAGCAAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                <  2:615578/146‑1 (MQ=255)
                                                                                                                            ttAATGCCTGAAAAACATGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 <  1:618703/145‑1 (MQ=255)
                                                                                                                              aaTGCCTAAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          <  1:746600/150‑1 (MQ=255)
                                                                                                                              aaTCCCTGAAAAACATGAAGGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                              <  2:954338/146‑1 (MQ=255)
                                                                                                                              aaTCCCTGAAAAACATCAATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          <  1:63353/150‑1 (MQ=255)
                                                                                                                               aTGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                         >  2:597605/1‑150 (MQ=255)
                                                                                                                                aatccttaaaaacCATGCATGAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          <  2:707331/141‑1 (MQ=255)
                                                                                                                                aaggcctaaaaaaCTTGAATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          <  1:769697/141‑1 (MQ=255)
                                                                                                                                 gCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                       >  2:606905/1‑150 (MQ=255)
                                                                                                                                   cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    >  1:402886/1‑151 (MQ=255)
                                                                                                                                     ttaaaaaaCTTCCACGAGCCAAAAAATAATATAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                      <  1:380359/145‑1 (MQ=255)
                                                                                                                                      tgaaaaaCATGCATGACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                        <  1:392318/143‑1 (MQ=255)
                                                                                                                                      tgaaaaaCATCCATGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                     <  2:337425/146‑1 (MQ=255)
                                                                                                                                      tatAAAACAGGAAGGACCCACAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                     <  1:1139066/147‑1 (MQ=255)
                                                                                                                                        aaaaaCAGGCAGGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    <  2:641530/147‑1 (MQ=255)
                                                                                                                                         aaaaatggattgAGCAAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                  <  1:689906/139‑1 (MQ=255)
                                                                                                                                          aaaCATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               <  2:341813/151‑1 (MQ=255)
                                                                                                                                           aaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               >  2:559805/1‑150 (MQ=255)
                                                                                                                                           aaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               >  1:1164232/1‑150 (MQ=255)
                                                                                                                                           aaCATGCATGAGCCACAAAATAATATAAAAAATACCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               >  2:311024/1‑150 (MQ=255)
                                                                                                                                            aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa              >  1:1052601/1‑150 (MQ=255)
                                                                                                                                             catgcatgAGCAAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa              <  2:956818/149‑1 (MQ=255)
                                                                                                                                                 catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg         >  2:694047/1‑150 (MQ=255)
                                                                                                                                                  atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg         >  2:22173/1‑149 (MQ=255)
                                                                                                                                                    gacccAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag       <  1:1187947/146‑1 (MQ=255)
                                                                                                                                                    gAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt      <  2:445438/150‑1 (MQ=255)
                                                                                                                                                      cccAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt      <  2:505761/147‑1 (MQ=255)
                                                                                                                                                        cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  1:1105758/1‑150 (MQ=255)
                                                                                                                                                          |                                                                                                                                                   
TTTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29336‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: