Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,200	0	G	A	41.8%	89.1 / 33.5	67	S217F (TCC→TTC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (37/2);  new base A (0/28);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 7.42e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCC  >  NC_000913/3556052‑3556349                                                                                                                                                      |                                                                                                                                                      cAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGa                                                                                                                                                      >  2:218188/1‑150 (MQ=255)   aCTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGaaa                                                                                                                                                    >  2:548329/1‑150 (MQ=255)        gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTcc                                                                                                                                              >  1:276385/1‑151 (MQ=255)          cGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTcccc                                                                                                                                            >  2:418236/1‑151 (MQ=255)              accaccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGcc                                                                                                                                         >  1:1180309/1‑150 (MQ=255)                caccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAgt                                                                                                                                      >  1:958102/1‑151 (MQ=255)                        cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGAt                                                                                                                              >  1:245597/1‑151 (MQ=255)                         aCTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGAtt                                                                                                                             >  1:631543/1‑151 (MQ=255)                           tGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTc                                                                                                                           >  1:414779/1‑151 (MQ=255)                                      ccccgtacacaaaaaagGCGTTTGGTGTTTTTTTCCCTTACAACTCAAAGCGCGACGGTTTTCCCTGCCCCCGGGACGCCCGGGAGTTAATAAATCTT‑TGTGACTTGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                                  <  2:80655/138‑1 (MQ=255)                                       cACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAAc                                                                                                               >  2:1077136/1‑151 (MQ=255)                                         cggacgacAAAAAGGCTTTGGGTAATATTTTCACTTTCGACTTCAAGCGAGGCGGTTTTCCCCGCCCCCGGGTCGCGGGGCAGGTTATGAATTTTCTGT‑TCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACAt                                                                                                             <  1:1008388/149‑1 (MQ=255)                                         ccGACGAAAAAAAGGCGTTCGGTGATTTTTCCACTTCCGACTTAAAGGGAGACGTTATCCCCCGCCCCCGGGCCGCGCGGCGGTTTATAAATTTTCTTT‑TCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                              <  2:352214/150‑1 (MQ=255)                                         ccGACAAAAAAAAGGTGTTCGGTGATTTTTTCCCTTCCGCCTTCAAGGGAGACGGTATTCCCCGCCCCCTGGCCGCGCGGCAGTTTATGAATTTTCTGT‑TCAGGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                              <  2:16105/150‑1 (MQ=255)                                          cgacgaCAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                            >  2:804804/1‑151 (MQ=255)                                           gacgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                           >  2:810591/1‑151 (MQ=255)                                              gacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc                                                                                                        >  1:1028625/1‑151 (MQ=255)                                               aaaaaaaaGCGTTCGGTGATTTTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCGGGTCGCGCGGCGGGTTATAAATTTTCTGT‑CCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                           <  1:842555/145‑1 (MQ=255)                                                 aaaaagGCGTTGGGTGATATTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGGCGCGCGGCAGTTTATGAATATTCTTT‑TCATGAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                         <  2:1136322/147‑1 (MQ=255)                                                 aaaaacCCGTTCGTTGTTATTTTCCCTTCCGACTTCAAGCAGGCCTTTTTTCCCCTTCCCCTGGTCGCGCGGCGGTTTTTGATTATTCTGT‑TCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                     <  2:278960/151‑1 (MQ=255)                                                       gCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                                >  1:843887/1‑150 (MQ=255)                                                         gTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                             >  1:79453/1‑151 (MQ=255)                                                          ttgggTTATTTTTTCCCTTTCGACTAAAAGCGAGACGGTTTTCCCCGGCCCTTGGTCGCGCGGCGGGTTATGAATATTTTTT‑TCAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                             <  2:1005283/147‑1 (MQ=255)                                                          ttCGGTGATTTTTTCCTTTTCGCCTTAAAGGGAGACGTTTTCCCCGGGCCCGTGGCCGCGCGGCAGTTTATGAATTTTCTTT‑TCAGGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                             <  2:1162635/150‑1 (MQ=255)                                                             ggTGATTTTTTCCTTTTCGACTTCAGGCGAGCCGTTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATAAATATTTTTT‑TCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                            <  1:324170/148‑1 (MQ=255)                                                               tGATTTTTTCCCTTTCGTCTTCAAGCGAGACGGTATTCCCCGGCCCGTGGTCGCGCGGCAGGTTATAAATATTCTGT‑TCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                        <  1:543851/150‑1 (MQ=255)                                                                   atTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                                   >  2:1063473/1‑151 (MQ=255)                                                                      ttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                                >  1:232585/1‑151 (MQ=255)                                                                      tccACTTTCGCTTTAAAGCGGGCCGTTTTCCCCCGGCCCCGGGTCGCGCGGCGGGTTATGAATATTCTGT‑TCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                                 <  2:1045890/148‑1 (MQ=255)                                                                         aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                              >  2:476206/1‑150 (MQ=255)                                                                           tttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGTGGCACACCAGCTAATAGAACTTCTCCAc                                                                            >  1:820376/1‑150 (MQ=255)                                                                           ttccGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCGGGTTATAAATATTCTGT‑TCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                                <  2:734808/143‑1 (MQ=255)                                                                           ttccGACTCCAGGCGAGACGTTATTCCCCGCCCCCTGGCCGCGCGGCGGGTTATAAATTTTTTGT‑TCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                             <  2:486202/146‑1 (MQ=255)                                                                            ttCGCCTTCAAGCGAGAGGTTTTTCCCCGGCCCCTGGTCGCGCGGCAGGTTATAAATATTCTTT‑TCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCAc                                                                            <  1:489422/149‑1 (MQ=255)                                                                             tCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                          >  2:122370/1‑150 (MQ=255)                                                                             tCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGACTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                          >  1:923514/1‑150 (MQ=255)                                                                              cGACTTCAGGCGAGACGATATTCCCCGCCCCCTGGTCGCGGGGCAGTTTATGAATATTCTGT‑TCAGGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                          <  1:541075/149‑1 (MQ=255)                                                                                    cAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                                  >  1:202295/1‑151 (MQ=255)                                                                                     aaGCGAGACGTTTTTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATATTCTTT‑TCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                                  <  2:1180279/150‑1 (MQ=255)                                                                                       gCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                               >  2:949879/1‑151 (MQ=255)                                                                                        cGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                              >  2:27084/1‑151 (MQ=255)                                                                                          agaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                            >  1:422392/1‑151 (MQ=255)                                                                                           gaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTc                                                           >  2:68352/1‑151 (MQ=255)                                                                                                    aCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                                  >  1:766079/1‑151 (MQ=255)                                                                                                     cccGGCCCCTGGTCGCGCGGCAGTTTATAAATATTTTGT‑CCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                                 <  2:206948/151‑1 (MQ=255)                                                                                                          ccctggcgcgcgcgGAGGGTTAGTAATATTTTGT‑TCAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                       <  2:780111/132‑1 (MQ=255)                                                                                                          ccccGGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGAAAAAACTCCCCGCCAGTGTAGCAATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGt                                            <  1:760576/151‑1 (MQ=255)                                                                                                           cccTGGTCGCGCGGCAGTTTATGAATATTCTGT‑TCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt                                           <  1:218193/151‑1 (MQ=255)                                                                                                              tGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                         >  1:566207/1‑150 (MQ=255)                                                                                                               ggTCGCGCGGCAGGTTATAAATATTTTGT‑TCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                        <  1:504428/150‑1 (MQ=255)                                                                                                                  cgcgcgGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgt                                    >  2:1146082/1‑151 (MQ=255)                                                                                                                  cgcgcgGCAGGTTATAAATTTTCTTT‑TCAGGGAGAAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                                <  2:636661/139‑1 (MQ=255)                                                                                                                      cgGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                 >  1:842509/1‑150 (MQ=255)                                                                                                                      cgGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                 >  1:674608/1‑150 (MQ=255)                                                                                                                           ggTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                            >  2:506669/1‑150 (MQ=255)                                                                                                                           ggTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                            >  1:706019/1‑150 (MQ=255)                                                                                                                           ggTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                            >  1:896120/1‑150 (MQ=255)                                                                                                                              tATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                        >  1:1172567/1‑151 (MQ=255)                                                                                                                                  aaTTTTCTGT‑TCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                      <  2:397105/149‑1 (MQ=255)                                                                                                                                  aaTTTTCTGT‑TCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                     <  1:604665/150‑1 (MQ=255)                                                                                                                                  aaTATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg                    >  2:1184564/1‑151 (MQ=255)                                                                                                                                      ttCTGT‑CCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc                 <  1:740654/150‑1 (MQ=255)                                                                                                                                       ttttgttaaggaaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc                 <  1:793332/138‑1 (MQ=255)                                                                                                                                         tGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg                >  2:521122/1‑148 (MQ=255)                                                                                                                                               aTGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc        <  1:1188761/151‑1 (MQ=255)                                                                                                                                                ggCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCg       <  2:809179/150‑1 (MQ=255)                                                                                                                                                 gCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc        <  1:49875/149‑1 (MQ=255)                                                                                                                                                     ggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGcc  >  1:463009/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGT‑TCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCC  >  NC_000913/3556052‑3556349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑