Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	362,830	C→A	C117F (TGT→TTT)	lacY ←	lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	362,830	0	C	A	97.4%	300.5 / 0.5	77	coding (350/1254 nt)	lacY	lactose permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (34/41);  minor base . (0/2);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

ATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTT  >  NC_000913/362685‑362975                                                                                                                                                   |                                                                                                                                                  attGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑Aaaaaaaaa                                                                                                                                               <  1:1160431/150‑1 (MQ=255)    gATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGcc                                                                                                                                            <  1:71257/150‑1 (MQ=255)       ggTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTa                                                                                                                                          <  1:317050/149‑1 (MQ=255)        gTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGAt                                                                                                                                       <  2:926453/151‑1 (MQ=255)         tGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGAt                                                                                                                                       >  1:570035/1‑150 (MQ=255)           aaCATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATaa                                                                                                                                     >  2:382031/1‑150 (MQ=255)            aCATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATaaa                                                                                                                                    <  2:75703/150‑1 (MQ=255)             cATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAAGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATa                                                                                                                                  <  1:1066830/151‑1 (MQ=255)              atgatgCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATac                                                                                                                                 <  1:157738/151‑1 (MQ=255)                gatgCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATacc                                                                                                                                <  2:533927/150‑1 (MQ=255)                 atgCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATaccac                                                                                                                              <  1:844417/151‑1 (MQ=255)                      gACAATAGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAACAAATTCGAAATTACTGCGACCGCTGGCTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAAAAAAAAGCCTAGATAAATACCACcaac                                                                                                                          <  2:650906/151‑1 (MQ=255)                         aaTCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATc                                                                                                                      <  2:389327/151‑1 (MQ=255)                         aaTCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGATGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATc                                                                                                                      <  2:1078055/151‑1 (MQ=255)                            cGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTTACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCAAGATAAATACCACCAACAATcta                                                                                                                    <  2:776711/150‑3 (MQ=255)                            cGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGa                                                                                                                    <  1:1004238/150‑1 (MQ=255)                            cGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGAt                                                                                                                   <  2:989132/151‑1 (MQ=255)                                gCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCct                                                                                                                >  1:597996/1‑150 (MQ=255)                                  acacaGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCctact                                                                                                             <  1:847863/151‑1 (MQ=255)                                   cacaGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCctacta                                                                                                            <  2:62960/151‑1 (MQ=255)                                   cacaGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCctact                                                                                                             <  2:69511/150‑1 (MQ=255)                                     caGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTaa                                                                                                           >  2:526741/1‑150 (MQ=255)                                        cgcCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtg                                                                                                       <  2:1185818/151‑1 (MQ=255)                                              gCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtat                                                                                                 >  1:584185/1‑151 (MQ=255)                                                cAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtattg                                                                                               >  1:1145207/1‑151 (MQ=255)                                                 aaCACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtattgt                                                                                              <  1:92812/151‑1 (MQ=255)                                                   cacaGCCAAACATCCGCGCGCGACCAAATTCGAAATTAATGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGttgtattgt                                                                                              >  2:162992/1‑149 (MQ=255)                                                     caGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAAc                                                                                           >  1:209552/1‑150 (MQ=255)                                                        ccAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTg                                                                                       <  2:717873/151‑1 (MQ=255)                                                          aaaCATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGc                                                                                     >  2:44320/1‑151 (MQ=255)                                                                 cgcgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGa                                                                                 >  2:81833/1‑149 (MQ=255)                                                                 cgcgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGa                                                                              <  2:1077299/151‑1 (MQ=255)                                                                   cgcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATa                                                                            <  2:774768/151‑1 (MQ=255)                                                                    gcgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATa                                                                            >  2:568028/1‑150 (MQ=255)                                                                     cgcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTAAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaaa                                                                          <  1:503467/151‑1 (MQ=255)                                                                      gcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaaaa                                                                         <  2:464590/151‑1 (MQ=255)                                                                      gcgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaaa                                                                          >  1:750568/1‑150 (MQ=255)                                                                       cgACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATaa                                                                           <  2:60535/148‑1 (MQ=255)                                                                           cAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATaaa                                                                    <  1:815454/151‑1 (MQ=255)                                                                           cAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATaa                                                                     >  1:936489/1‑150 (MQ=255)                                                                           cAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATa                                                                      <  1:557838/149‑1 (MQ=255)                                                                            aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATaa                                                                     >  2:395337/1‑149 (MQ=255)                                                                            aaaTTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAg                                                                   <  1:537529/151‑1 (MQ=255)                                                                                tCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAAc                                                                <  2:689910/150‑1 (MQ=255)                                                                                 cGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACg                                                               <  1:512123/150‑1 (MQ=255)                                                                                    aaTTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGgcgc                                                           >  1:583440/1‑151 (MQ=255)                                                                                    aaTTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGgcgc                                                           <  2:1162180/151‑1 (MQ=255)                                                                                      ttACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCa                                                          >  1:142023/1‑150 (MQ=255)                                                                                       tACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCaa                                                         >  1:832462/1‑150 (MQ=255)                                                                                        aCTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCaaa                                                        >  1:842676/1‑150 (MQ=255)                                                                                        aCTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAAc                                                       >  1:923674/1‑151 (MQ=255)                                                                                           gCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACa                                                      <  2:596312/149‑1 (MQ=255)                                                                                            cGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATc                                                    >  2:758304/1‑150 (MQ=255)                                                                                            cGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATc                                                    >  2:921917/1‑150 (MQ=255)                                                                                            cGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAA‑AAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCa                                                   <  1:962115/150‑1 (MQ=255)                                                                                                    gACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATg                                           >  1:915065/1‑151 (MQ=255)                                                                                                       tttCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCg                                        >  1:673597/1‑151 (MQ=255)                                                                                                         tctcAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCgg                                       >  2:870511/1‑150 (MQ=255)                                                                                                         tctcAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCgg                                       <  2:640673/150‑1 (MQ=255)                                                                                                            cAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGta                                     <  2:840297/149‑1 (MQ=255)                                                                                                                  aTGCCTCTACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCca                             <  1:125238/151‑1 (MQ=255)                                                                                                                       tctACTGCCGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCAcagc                         >  1:653419/1‑150 (MQ=255)                                                                                                                        ctACTGCTGGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCAcagc                         <  1:834526/149‑1 (MQ=255)                                                                                                                           ctgctgGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAgg                      >  2:97365/1‑149 (MQ=255)                                                                                                                           ctgctgGCGCACCGGCGTT‑AAAA‑AAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAgg                      <  1:452602/148‑1 (MQ=255)                                                                                                                            tgctgGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTAt                   >  2:828753/1‑151 (MQ=255)                                                                                                                             gctgGCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTAtt                  >  2:827678/1‑151 (MQ=255)                                                                                                                                ggCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgc               <  1:526756/151‑1 (MQ=255)                                                                                                                                ggCGCACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTg                <  1:946146/150‑1 (MQ=255)                                                                                                                                 gcgcACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcg              <  2:1020939/151‑1 (MQ=255)                                                                                                                                   gcACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCa            <  1:915214/151‑1 (MQ=255)                                                                                                                                    cACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcgc             >  1:643092/1‑149 (MQ=255)                                                                                                                                    cACCGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTgcgc             >  1:1087736/1‑149 (MQ=255)                                                                                                                                      ccGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGcc         >  1:909182/1‑151 (MQ=255)                                                                                                                                      ccGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGc          <  2:135766/150‑1 (MQ=255)                                                                                                                                       cGGCGTT‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGccc        >  2:831848/1‑151 (MQ=255)                                                                                                                                            tt‑AAAAAAAAAGCCTAGATAAATACCACAAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGtt   >  2:449160/1‑151 (MQ=255)                                                                                                                                             t‑AAAAAAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGttt  >  2:18195/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTT‑AAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTGTATTGTAACAGTGGCCCGAAGATAAAAATAAAGAACGGCGCAAACATCACTAACATGCCGGTAATAATCCACAGCAGGTATTTGCGCAGCCCGAGTTT  >  NC_000913/362685‑362975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑