Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,613	G→A	S45L (TCA→TTA)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,613	0	G	A	100.0%	259.9 / NA	66	S45L (TCA→TTA)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/31);  total (35/31)

TGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTC  >  NC_000913/2725470‑2725759                                                                                                                                                |                                                                                                                                                   tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGa                                                                                                                                              <  2:220006/150‑1 (MQ=255) tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGa                                                                                                                                              <  1:801270/150‑1 (MQ=255)  gCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGaa                                                                                                                                             <  2:492047/150‑1 (MQ=255)  gCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGaa                                                                                                                                             <  1:349354/150‑1 (MQ=255)    gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                           >  1:557115/1‑150 (MQ=255)    gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                           >  1:1199092/1‑150 (MQ=255)     aCCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                           >  1:536301/1‑149 (MQ=255)      ccATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAg                                                                                                                                         <  2:931181/150‑1 (MQ=255)           tttATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGGAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGAc                                                                                                                                    >  1:682071/1‑150 (MQ=255)           tttATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGAc                                                                                                                                    >  2:104240/1‑150 (MQ=255)              aTCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACAt                                                                                                                                  >  1:479626/1‑149 (MQ=255)              aTCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATa                                                                                                                                 <  1:508468/150‑1 (MQ=255)                cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                               >  1:470609/1‑150 (MQ=255)                cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                               >  1:980046/1‑150 (MQ=255)                 ggCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                               <  1:1138235/149‑1 (MQ=255)                   cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAAt                                                                                                                            >  1:956456/1‑150 (MQ=255)                       gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAc                                                                                                                       >  2:541839/1‑151 (MQ=255)                        cGGCAAAATCCCCAGCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAGAAACGCCTGCTGTTTGTAGGAGTTGAGTCGTCGTGTTCCCTGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGATTAGACATAGAAATc                                                                                                                           <  2:322930/146‑1 (MQ=255)                         ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAcc                                                                                                                      >  1:692086/1‑150 (MQ=255)                         ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAcc                                                                                                                      >  1:299649/1‑150 (MQ=255)                         ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCa                                                                                                                     <  1:966408/151‑1 (MQ=255)                          gCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCAc                                                                                                                    >  2:630347/1‑151 (MQ=255)                          gCCAAAGAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCAc                                                                                                                    <  1:106573/151‑1 (MQ=255)                            cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTc                                                                                                                  >  1:835490/1‑151 (MQ=255)                                 aGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAcc                                                                                                              <  2:33742/150‑1 (MQ=255)                                 aGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAcc                                                                                                              <  1:1179534/150‑1 (MQ=255)                                     atccGCCTATTGGGCGCATCAGGAATGCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAtt                                                                                                         <  1:1069985/149‑1 (MQ=255)                                       ccGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAc                                                                                                       >  1:882891/1‑151 (MQ=255)                                           cTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg                                                                                                    >  2:896817/1‑150 (MQ=255)                                               tGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAag                                                                                                 >  1:867105/1‑149 (MQ=255)                                                gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAgg                                                                                              <  1:300992/151‑1 (MQ=255)                                                    gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGcccc                                                                                          >  2:193698/1‑151 (MQ=255)                                                      aTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCca                                                                                        <  2:228747/151‑1 (MQ=255)                                                      aTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCca                                                                                        <  1:1201615/151‑1 (MQ=255)                                                               cccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGcc                                                                                <  1:769038/150‑1 (MQ=255)                                                                ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGccc                                                                               >  1:932321/1‑150 (MQ=255)                                                                 cGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCa                                                                              >  1:1147105/1‑150 (MQ=255)                                                                          aaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcga                                                                    <  1:768933/151‑1 (MQ=255)                                                                           acacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgac                                                                   >  1:277039/1‑151 (MQ=255)                                                                                      ttGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCact                                                         <  1:204141/150‑1 (MQ=255)                                                                                      ttGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCact                                                         >  1:459062/1‑150 (MQ=255)                                                                                        gtagtaGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactact                                                      >  2:515572/1‑151 (MQ=255)                                                                                            tagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTc                                                  >  2:287700/1‑151 (MQ=255)                                                                                              gtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAg                                                <  1:358850/151‑1 (MQ=255)                                                                                                tGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGt                                               <  2:687754/150‑1 (MQ=255)                                                                                                     cgtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTg                                         <  1:156033/151‑1 (MQ=255)                                                                                                      gtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGt                                        >  1:1094021/1‑151 (MQ=255)                                                                                                      gtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGt                                        >  1:327992/1‑151 (MQ=255)                                                                                                         gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTg                                     >  2:517231/1‑151 (MQ=255)                                                                                                         gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTg                                     <  1:621083/151‑1 (MQ=255)                                                                                                              cccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                >  2:665295/1‑151 (MQ=255)                                                                                                               ccGGAAGGGACGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                >  2:188636/1‑150 (MQ=255)                                                                                                                  gaagggaagAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                            <  1:885736/151‑1 (MQ=255)                                                                                                                   aagggaagAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGt                           <  2:905768/151‑1 (MQ=255)                                                                                                                   aagggaagAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGt                           >  2:614909/1‑151 (MQ=255)                                                                                                                    agggaagAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTa                          <  2:956025/151‑1 (MQ=255)                                                                                                                       gaagaaGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACtt                       <  1:753245/151‑1 (MQ=255)                                                                                                                        aagaagATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt                      <  2:735134/151‑1 (MQ=255)                                                                                                                              aTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgcca                <  2:408648/151‑1 (MQ=255)                                                                                                                               tgtgGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccat               >  1:574708/1‑151 (MQ=255)                                                                                                                                 tgGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgc             >  1:26518/1‑151 (MQ=255)                                                                                                                                   ggCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgcca           <  1:409398/151‑1 (MQ=255)                                                                                                                                    gCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat          >  1:521891/1‑151 (MQ=255)                                                                                                                                       aaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTAt       >  1:383016/1‑151 (MQ=255)                                                                                                                                           agagTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtc    <  1:162485/150‑1 (MQ=255)                                                                                                                                            gagTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc  >  1:853923/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                   TGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTC  >  NC_000913/2725470‑2725759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑