Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 345,328 | 0 | C | A | 42.4% | 89.5 / 31.2 | 59 | intergenic (+337/‑76) | yahL/yahM | uncharacterized protein/uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base C (30/4); new base A (0/25); total (30/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.84e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTG > NC_000913/345179‑345467 | aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATc > 2:903191/1‑150 (MQ=255) aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATc > 1:457231/1‑150 (MQ=255) aaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTACATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCaa < 2:700882/151‑1 (MQ=255) tGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAAcc > 1:133418/1‑151 (MQ=255) tctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGcc > 1:1076492/1‑151 (MQ=255) gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCAtt > 1:758146/1‑148 (MQ=255) tgtaattgttttgtttAACATTCATATAAAAAAAATCAAAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTCCGCATCGCACTCAAGCCTGAAAAAAAATATGCTGTAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAgg < 2:283534/143‑1 (MQ=255) attgtgTTGGTTGACATTAATATGAAAAAAACCATAATCCCATCATGTTTGTGTGGGTTCTTTTCTTTATCTTCCCAACCCCACTCAGGCCTGAAAAAAAATATGCTGAAAGCCTA‑ATAAAAAAAACCGCCATTAGCTCATCAGGa < 1:757062/144‑1 (MQ=255) aaTGTGTTGTTTAACATTCATATAAAAAAAATAATAATCCCATCATTTTTGTGTGGGGTTTTTTCTGTATCTTACGCACCCCACTCAACCCTGAAAAAAAATAGGCTGTAAGGCTA‑ATACAAAAAACCGCCATTAGCTCATCAGGa < 1:159198/146‑1 (MQ=255) tgtTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac > 1:849858/1‑151 (MQ=255) tggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac > 2:61052/1‑151 (MQ=255) tggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac > 1:428249/1‑151 (MQ=255) tggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac > 2:703099/1‑151 (MQ=255) ttgACATTCATATAAAAAAAATCAAAATCCCATCATTTTTGTGTGGGTTTTTTTCTGTATCTTACGCACCCCACTAAAGCCTGAAAAAAAATATGCTGAAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACa < 1:1001611/149‑1 (MQ=255) tgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc > 2:17480/1‑151 (MQ=255) gACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc > 2:637718/1‑150 (MQ=255) aTTAATATAAAAAAAATCATAAGTCCATCATGTTTGGGTGGGGTCTTTTCTGTATCTTCCCCACCGCACTCAAGCCTGACAAAAAATATGCAGTAAGGCTA‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAg < 1:98325/149‑1 (MQ=255) ttCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt > 1:172353/1‑151 (MQ=255) tCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 1:236394/1‑151 (MQ=255) tCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt > 2:1027285/1‑151 (MQ=255) atatGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt > 1:157082/1‑151 (MQ=255) atatAAAAAAAATCATAATCCCATCATTTTTGTGTGGGTTCTTTTCTGTATCTTACGCACCGCACTCAAGCCTGACAAAAAATATGCTGAAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt < 2:92084/149‑1 (MQ=255) gAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATtgttgt > 1:166919/1‑150 (MQ=255) aaaaaaaTAAAAATCCCATCATGTTTGTGTGGGTTCTTTTCTGTATCTTCCGCATCGCACACAAGCCTGACAAAAAATATGCTGAAAGGCTC‑ATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAt < 2:391599/151‑1 (MQ=255) aaaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATg > 1:1156994/1‑151 (MQ=255) aaaaaTGATAATCCCAGAATTTTTGGGTGGGTTCTTTTCTGTTTGTACCGATCCCCACTCGGGCGTGAAAGAAATTAGGCT‑TAAGGCCCGATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg < 1:136671/151‑1 (MQ=255) aaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt > 2:1027577/1‑151 (MQ=255) cATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACggg > 1:563764/1‑151 (MQ=255) atatTTCCACCATGTTTGGGGGGGGTTTTTTTTGTCTCTTACGAATCGCACACAACTTTGACAAAAAATATGCTGTAAGGCTT‑GTAAAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACggg < 2:804866/150‑1 (MQ=255) aaTTCCATCATTTTGGTGTGGGTTTTTTTCTTTATCTTACCCACCCCCCCAAAGCCTGACAAAAAATTTGTTGTAAGGCTA‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgggg < 1:628495/149‑1 (MQ=255) ttCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg > 1:876061/1‑151 (MQ=255) atcatGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGc > 2:919278/1‑151 (MQ=255) catGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt > 2:816524/1‑151 (MQ=255) tgtgtgGGGTTTTTTCTGTATCTTCCGCATCGCCCTCAGGCCTGAAAAAAAATATGCTGTAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATg < 2:299993/150‑1 (MQ=255) tgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGt > 2:315530/1‑151 (MQ=255) ggttttttttCTGTATTTTCCCCATCCCACTGAAGCCTTACAGAAAAGATGGTGTAAGGCCC‑TGAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATg < 2:1066922/139‑1 (MQ=255) ggggTTTTTTTTTTATCTTACCCATCCCCCCCAAGCCTGAAAAAAAATATGCTGTAAGGCTA‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGc < 1:1109257/147‑1 (MQ=255) gTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCa > 2:506610/1‑150 (MQ=255) ttCTGTTTCTTACGCATCACACCAACGCTTAACAAAAAATATCCGTTAGGGCTA‑ATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACt < 2:822365/148‑1 (MQ=255) ttCTGTATCTTCCCAACCGCACTAAAGCCTGAAAAAAAATATGCTGAAAGCCTA‑ATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACttt < 2:356556/150‑1 (MQ=255) gTATCTTCCGCATCGCACTCAAGCCTAAAAAAAAATATGCTGTAAGGCTA‑ATATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTCCGAGGCCTCGATGGCGGTCCAACTTTTc < 1:223038/148‑1 (MQ=255) gTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaa > 1:618161/1‑150 (MQ=255) ttACGCATCGCACTAAAGCCTGAAAAAAAATATGTTGTAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa < 1:551081/146‑1 (MQ=255) ttACGCATCGCACCCAAGCCAGAAAAAAAATATGCTGAAGGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa < 1:424214/147‑1 (MQ=255) cATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTg > 1:1134609/1‑150 (MQ=255) cGCACTCAAGCCTGAAAAAAAATATGCTGAAAGGCTA‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTg < 2:731594/147‑1 (MQ=255) aggcataaaaaaaatTATGTTGAAAGGCAC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACATCCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATc < 2:524146/138‑1 (MQ=255) aaGCCTAAAAAAAAATATGTTATAGGCCTC‑ATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc < 1:707836/150‑1 (MQ=255) gccagaaaaaaaaTATGCTGTAAGGCTC‑ATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGAt < 2:532371/137‑1 (MQ=255) ccctaaaaaaaaaTATGCTGAAGGCCTA‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCt < 1:709086/142‑1 (MQ=255) ccTGAAAAAAAATATGTTGTAAGGCTC‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt < 2:428255/151‑1 (MQ=255) tGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttat > 1:979468/1‑151 (MQ=255) aaaaaaTATGCTGAAAGGCTA‑ATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttat < 2:1071459/145‑1 (MQ=255) aGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc > 1:712466/1‑151 (MQ=255) atGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTg > 1:433800/1‑151 (MQ=255) gttgaagggCTA‑AAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa < 1:393526/144‑1 (MQ=255) tGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa > 2:754470/1‑150 (MQ=255) tGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa > 2:294723/1‑150 (MQ=255) aaGGCTC‑ATATCAAAAACCGCCTTTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg < 1:113787/151‑1 (MQ=255) aaGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg > 1:284572/1‑151 (MQ=255) ggCTA‑ATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg < 2:788484/149‑1 (MQ=255) | AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTC‑ATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTG > NC_000913/345179‑345467 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |