Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,613	G→A	S45L (TCA→TTA)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,613	0	G	A	100.0%	239.5 / NA	61	S45L (TCA→TTA)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/34);  total (27/34)

TTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTAT  >  NC_000913/2725469‑2725754                                                                                                                                                 |                                                                                                                                              ttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAg                                                                                                                                          <  2:1099230/150‑1 (MQ=255)  tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGa                                                                                                                                         <  1:132261/150‑1 (MQ=255)    cGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAAc                                                                                                                                       <  2:230920/150‑1 (MQ=255)    cGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                      >  1:1134979/1‑151 (MQ=255)     gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                      <  1:658580/150‑1 (MQ=255)      aCCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACg                                                                                                                                      >  2:660620/1‑149 (MQ=255)          tGTTTTTCGAGTATGCGGCCAAATAGCCAACCGCCTATTGGGCACATCAGGAATCCCGCAGCAAGAACACCTGCTGTCTGTAGTAGTTGAGTCGTCGTGTTCCCGCAAGGGAAGAAGATTTGGGCAAAGTAGAGTAAACAGAAc                                                                                                                                       <  1:1078617/144‑1 (MQ=255)            tttATCGGCTAAGCGGCCAAATAGCCAGCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGAc                                                                                                                               <  1:775161/150‑1 (MQ=255)             ttATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACa                                                                                                                              <  2:571696/150‑1 (MQ=255)              tATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACAt                                                                                                                             <  1:285994/150‑1 (MQ=255)                 cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                          <  2:254772/150‑1 (MQ=255)                 cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGa                                                                                                                          >  2:938117/1‑150 (MQ=255)                    caaggCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGaaag                                                                                                                       <  1:817099/146‑2 (MQ=255)                    cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATc                                                                                                                      >  2:426356/1‑151 (MQ=255)                      aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCg                                                                                                                     >  1:67850/1‑150 (MQ=255)                      aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGa                                                                                                                    <  2:924646/151‑1 (MQ=255)                          ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAcc                                                                                                                 >  2:815986/1‑150 (MQ=255)                          ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAcc                                                                                                                 >  1:1121523/1‑150 (MQ=255)                          ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAAcc                                                                                                                 >  2:1093847/1‑150 (MQ=255)                           gCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCAc                                                                                                               <  2:738355/151‑1 (MQ=255)                            ccAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACt                                                                                                              <  2:491409/151‑1 (MQ=255)                             cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTc                                                                                                             >  2:362675/1‑151 (MQ=255)                                aTAGCCACCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                                                                                                          <  1:221264/151‑1 (MQ=255)                                aTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                                                                                                          <  1:1074897/151‑1 (MQ=255)                                      aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAtt                                                                                                    <  1:247904/151‑1 (MQ=255)                                      aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGAtt                                                                                                    <  1:415536/151‑1 (MQ=255)                                          gCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCt                                                                                                >  1:402993/1‑151 (MQ=255)                                           ccTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg                                                                                               >  2:41947/1‑151 (MQ=255)                                                 gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAgg                                                                                         <  1:967564/151‑1 (MQ=255)                                                 gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAgg                                                                                         <  1:469323/151‑1 (MQ=255)                                                         cAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGCAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCcac                                                                                  >  2:632588/1‑150 (MQ=255)                                                          aGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAAAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCcaca                                                                                 >  1:1094085/1‑150 (MQ=255)                                                              aTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGc                                                                            <  1:113403/151‑1 (MQ=255)                                                                 ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCa                                                                         <  2:317700/151‑1 (MQ=255)                                                                   gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa                                                                        >  2:346994/1‑150 (MQ=255)                                                                         aaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcggc                                                                 >  1:494211/1‑151 (MQ=255)                                                                          aaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcggcg                                                                >  2:79323/1‑151 (MQ=255)                                                                           aaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcggcg                                                                >  2:400979/1‑150 (MQ=255)                                                                           aaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcga                                                               <  1:324160/151‑1 (MQ=255)                                                                            acacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgac                                                              >  2:158526/1‑151 (MQ=255)                                                                                ctgctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                          >  1:517687/1‑151 (MQ=255)                                                                                        tGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                  >  1:428740/1‑151 (MQ=255)                                                                                           agtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTg                                               >  2:1074835/1‑151 (MQ=255)                                                                                             tagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTc                                             >  1:744670/1‑151 (MQ=255)                                                                                                 tGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGt                                          <  1:998815/150‑1 (MQ=255)                                                                                                  gAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGttt                                        >  2:172764/1‑151 (MQ=255)                                                                                                  gAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGttt                                        <  1:844447/151‑1 (MQ=255)                                                                                                      cgtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTg                                    <  2:145635/151‑1 (MQ=255)                                                                                                         cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                 <  1:373068/151‑1 (MQ=255)                                                                                                            gtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGCCGAGTATCACTACTTGTCAGTTTGCTGTCTGcc                              <  1:78473/151‑1 (MQ=255)                                                                                                            gtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGc                               <  1:938094/150‑1 (MQ=255)                                                                                                                ccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                           >  1:440233/1‑150 (MQ=255)                                                                                                                 cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTAc                         >  2:1049415/1‑151 (MQ=255)                                                                                                                          agaagaTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                <  2:1118013/151‑1 (MQ=255)                                                                                                                          agaagaTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                <  1:67708/151‑1 (MQ=255)                                                                                                                             agaTGTGGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgc             <  1:118349/151‑1 (MQ=255)                                                                                                                                  tgGGCAAAGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgc        >  2:453191/1‑151 (MQ=255)                                                                                                                                       aaaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCAtt    >  2:209350/1‑150 (MQ=255)                                                                                                                                       aaaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTa   <  2:1110736/151‑1 (MQ=255)                                                                                                                                        aaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTAt  <  2:3563/151‑1 (MQ=255)                                                                                                                                        aaGTAGAGTAAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGCCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTAt  <  2:95425/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                              TTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTAT  >  NC_000913/2725469‑2725754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑