Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	41.5%	135.5 / 42.1	82	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (35/13);  new base A (0/34);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 1.54e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29341‑29633                                                                                                                                                    |                                                                                                                                                    gttgttTTAAGGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACaaaa                                                                                                                                                 <  1:1145212/150‑1 (MQ=255)     ttttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAaataat                                                                                                                                             <  1:674509/150‑1 (MQ=255)      tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAaataat                                                                                                                                             <  2:1076083/149‑1 (MQ=255)        tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                          >  1:597585/1‑150 (MQ=255)        tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                          <  1:250211/150‑1 (MQ=255)            gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                      <  1:547965/150‑1 (MQ=255)             tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    >  1:1074524/1‑151 (MQ=255)                aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                 <  1:425245/151‑1 (MQ=255)                  tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                               >  1:355165/1‑151 (MQ=255)                   ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                               <  2:1139837/150‑1 (MQ=255)                   ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                               <  2:736043/150‑1 (MQ=255)                   ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGc                                                                                                                               <  1:266974/150‑1 (MQ=255)                       ccttttGGTCCATTTTTTTCTGCTCGTTTTTATTTCTTGCAATTTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGTTTAGATGATTTTTTTGTCGTTTAATG‑CTTGAAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                            <  1:519086/146‑1 (MQ=255)                        cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                          >  2:580805/1‑150 (MQ=255)                         aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                          >  2:406565/1‑149 (MQ=255)                         aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                          >  1:1007866/1‑149 (MQ=255)                            tgttccttttttttCTGCTCTTTTTTTTTTCATGCAATTTTCTTGCTGCGCAAGCTTTTTCAAA‑AACAGGTTAGATAATTTTTTTGTCGTTTAATC‑CCTGAAAAACATGCATGACCCAAAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                      <  1:473598/142‑1 (MQ=255)                                   ttttttttttttttttttttttTAATGAATTTTTCTGGCGCCGCAGGCTTTTCCAAG‑AACAGTTAAAATTTTTTTTTTGTCGTTTAAGG‑CCTAAAAAACATGAAGGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACtttt                                                                                                               <  1:608170/136‑1 (MQ=255)                                   cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                               >  2:1148219/1‑150 (MQ=255)                                    ttttttCTGCTCTTTTTTTTTTCATGCATTCTTTTTGCTGCGCAAGCTTTTTCAAA‑AACAGGTTAGATGATTTTTTTGTCGTTTAATG‑CCTAAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTa                                                                                                              <  2:772261/150‑1 (MQ=255)                                            gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                      >  2:772255/1‑150 (MQ=255)                                              tCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                   >  1:750767/1‑151 (MQ=255)                                                    ttATTTCATGCATTCTTCTTGCTGCGCAAGCGTTTTCAAG‑AACAGTTAAAATGTTTTTTTTGTCGCTTACTG‑CCTGAAAAAAATGCATGAGCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                              <  1:932964/150‑1 (MQ=255)                                                       tttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                           >  2:832788/1‑150 (MQ=255)                                                       tttCATGAATTCTTCTTGCTGCGAAGGCTTTTTCCAA‑AACAGTTTAGATGATTTTTTTGTCGTTTAATG‑CCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                            <  2:926990/149‑1 (MQ=255)                                                        ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                         >  2:257158/1‑151 (MQ=255)                                                        tccATGCAATCTTCTTGCTGCGAAAGCTTTTTCCAA‑AACAGGTTAGATTATTTTTTTGTCGCTAAATG‑CCTGAAAAACATGCATGAGCCAAAAAATAAAATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                          <  2:1153838/148‑1 (MQ=255)                                                             gCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                    >  2:828930/1‑151 (MQ=255)                                                              cAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                   >  2:741500/1‑151 (MQ=255)                                                                aTCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                 >  2:1168199/1‑151 (MQ=255)                                                                 tcttttTGCTGCGAAAGCTTTTTAGAA‑AAAGGTTTAAATTATTTTTTTGCCATTAAATC‑CCTAAAAAACTTGCGTAACCAAAAAAAAATTAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                  <  1:214111/149‑1 (MQ=255)                                                                       tgctccGCAAGCTTTTTCAAA‑AAAAGTTAAGATGTTTTTTTTGTCGTTTAATG‑CCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  1:874096/150‑1 (MQ=255)                                                                        gctgcGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                          >  2:1056207/1‑150 (MQ=255)                                                                        gctgcGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                          >  2:563192/1‑150 (MQ=255)                                                                            cgcAAGCTTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CTTTAAAAACATGCATGAGCAAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                        <  2:765919/148‑1 (MQ=255)                                                                            cgcAAGCTTTTTCCAA‑AACGGGTTAGATGTTTTTTTTGTCGTTTAATG‑CCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  2:1112011/145‑1 (MQ=255)                                                                             gcAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                    >  1:1010335/1‑151 (MQ=255)                                                                              cAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                   >  1:805101/1‑151 (MQ=255)                                                                               aggctttttCAAG‑AACAGGTTAGATGTTTTTTTTCTCGTTTAATG‑CCTAAAAAACATGAAGGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                      <  1:317149/142‑1 (MQ=255)                                                                               aaGCTTTTTCAAA‑AACAGTTTAGTTTTTTTTTTTGTCTTTTATTC‑CTTTAAAAACATGCAGGACCAAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                  <  1:685959/151‑1 (MQ=255)                                                                               aaGCTTTTCCCAA‑AACGGTTTAGATGTTTTTTTTGCCGCTTAAGG‑CCTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                             <  2:571571/140‑1 (MQ=255)                                                                               aaGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                  >  2:820754/1‑151 (MQ=255)                                                                               aaGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                  >  1:472152/1‑151 (MQ=255)                                                                                 gCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                >  1:1084395/1‑151 (MQ=255)                                                                                 agcttttCCAAAAACAGTTTAGATGTTTTTTTTGTCTTTTAATC‑CCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                 <  1:370808/148‑1 (MQ=255)                                                                                   gTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               >  2:298510/1‑150 (MQ=255)                                                                                    ttttCCAA‑AACAGGTTAGATGTTCTTTTTGTCGTTAAATG‑CCTAAAAAACATGCATGCCCCAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                 <  2:1018652/147‑1 (MQ=255)                                                                                    tttcCCAG‑AACAGGTTAGATTATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGACCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               <  2:600030/149‑1 (MQ=255)                                                                                     tttCAAA‑AACAGGTTAGATGATTTTTTTGTCGCTTAATG‑CTTGAAAAACATGCATGAGCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                             <  2:593507/150‑1 (MQ=255)                                                                                       tCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  2:664045/1‑149 (MQ=255)                                                                                        ccAG‑AACAGTTAAAATGTTTTTTTTGT‑GCTTAAAGCCCTAAAAACCATGTATGACCCAAAAAATAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            <  1:688989/148‑1 (MQ=255)                                                                                         cAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            >  1:296647/1‑147 (MQ=255)                                                                                           g‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                      >  2:550139/1‑151 (MQ=255)                                                                                             aaCAGGTTAAATGTTTTTTTTGTCGTTTAATG‑CCTGTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                       <  2:37663/149‑1 (MQ=255)                                                                                              aCAGTTAAGATGATTTTTTTGTCGTTAAAGG‑CCTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGTGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                    <  2:249243/151‑1 (MQ=255)                                                                                               cAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                    >  1:474931/1‑150 (MQ=255)                                                                                                aGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                  >  2:534149/1‑151 (MQ=255)                                                                                                      gatgatTTTTTTGTGGTTAAATG‑CTGGAAAAACACGCAGGGCCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  2:365568/147‑1 (MQ=255)                                                                                                       atgatCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                            >  1:128486/1‑150 (MQ=255)                                                                                                         gatCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                         >  2:121459/1‑151 (MQ=255)                                                                                                           tttttttGTCGTTTAATG‑CCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                            <  1:580794/144‑1 (MQ=255)                                                                                                             tttttGTCTTTTAATC‑CCTTAAAAACATGAAGGACCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                      <  1:493768/150‑1 (MQ=255)                                                                                                             tttttGTCTCTTAATG‑CTTAAAAAACATGAATGACCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                      <  1:503334/150‑1 (MQ=255)                                                                                                             tttttGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                     >  2:161594/1‑151 (MQ=255)                                                                                                             tttttGTCACTTAATG‑CCTAAAAAACATCCATGACCCACAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       <  1:921909/149‑1 (MQ=255)                                                                                                                    cgtttAATC‑CCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                               <  2:183716/147‑1 (MQ=255)                                                                                                                    cgttaaaTG‑CCTAAAAACCATGCAGGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 <  1:39352/143‑1 (MQ=255)                                                                                                                    cattaaaTG‑CCTAAAAAACATGAATGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                  <  1:140836/142‑1 (MQ=255)                                                                                                                       ttAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           >  1:751808/1‑151 (MQ=255)                                                                                                                       ttAATC‑CCTAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                             <  1:988736/149‑1 (MQ=255)                                                                                                                         aaTG‑CCTGTAAAACATGCATGCGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           <  2:123962/149‑1 (MQ=255)                                                                                                                          aTG‑CCTGAAAAACATGCATGGGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                        <  2:228644/151‑1 (MQ=255)                                                                                                                              cctaaaaaaCAGGCATGAGCCAAAAAATAATATAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                      <  1:590684/145‑1 (MQ=255)                                                                                                                              ccTGTAAAACAGGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                      <  1:157626/150‑1 (MQ=255)                                                                                                                               cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    >  2:940938/1‑151 (MQ=255)                                                                                                                                tGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                   >  2:680412/1‑151 (MQ=255)                                                                                                                                   aaaaCATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                  <  2:817699/149‑1 (MQ=255)                                                                                                                                   aaaaCATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                   <  2:181497/148‑1 (MQ=255)                                                                                                                                    aaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               >  1:8185/1‑151 (MQ=255)                                                                                                                                           catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg         >  2:972032/1‑150 (MQ=255)                                                                                                                                           catgAGCAAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga        <  1:164468/151‑1 (MQ=255)                                                                                                                                             tgAACCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga        <  1:810847/149‑1 (MQ=255)                                                                                                                                                  cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  1:57875/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAG‑AACAGGTTAGATGATCTTTTTGTCGCTTAATG‑CCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29341‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑