Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	51.3%	41.4 / 109.7	74	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (7/29);  new base T (38/0);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 2.67e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-09

AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAG  >  NC_000913/3206286‑3206579                                                                                                                                                      |                                                                                                                                                  aGTGTATTTCGCAAAACATTGCCATTCCCTGCTATGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTcc                                                                                                                                                  <  2:227841/151‑1 (MQ=255)  gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                 <  2:1096034/151‑1 (MQ=255)     tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGa                                                                                                                                               >  1:956259/1‑150 (MQ=255)            cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAaaaa                                                                                                                                           >  2:783152/1‑147 (MQ=255)            cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                                       <  1:83277/151‑1 (MQ=255)               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAaaaaaaaa                                                                                                                                     >  2:251469/1‑150 (MQ=255)                aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGacaaaaacaaaa                                                                                                                                    >  1:511115/1‑150 (MQ=255)                 cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAaaaaaa                                                                                                                                   >  2:278161/1‑150 (MQ=255)                    tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑g                                                                                                                               <  2:848754/150‑1 (MQ=255)                     gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑gg                                                                                                                              <  1:1130769/150‑1 (MQ=255)                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAACT‑TGAGtt                                                                                                                          >  2:948656/1‑151 (MQ=255)                         ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGATTGTGGCGGGGAGTTTTTCAAGAAAAAAACAAAAAAT‑GGAGtt                                                                                                                          >  2:748286/1‑150 (MQ=255)                           cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGcc                                                                                                                       <  1:883736/151‑1 (MQ=255)                           cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAATAAAAAAAAT‑CGAGATGcc                                                                                                                       >  2:466823/1‑151 (MQ=255)                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACAAATAAAAAAAAT‑GGGGtt                                                                                                                          >  1:620036/1‑145 (MQ=255)                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAATTTTTCAAACAAATAAAAAAAAT‑GGAGTTggc                                                                                                                       >  2:300447/1‑146 (MQ=255)                                 tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAACT‑GGAGTTGCCat                                                                                                                     >  2:543962/1‑147 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACA‑TGAGGTGTCatg                                                                                                                    >  2:609437/1‑145 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGc                                                                                                              <  1:1027366/151‑1 (MQ=255)                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCg                                                                                                             <  2:717921/151‑1 (MQ=255)                                      cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTTAGAAAAAAACAAAAAAT‑AGGGTTGcccttgtgag                                                                                                               >  1:224666/1‑140 (MQ=255)                                       gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAAAAAAAACAAAAAAT‑GGAGGTGTCATGATGAGCg                                                                                                             >  2:245159/1‑149 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGaaa                                                                                                          <  1:37980/150‑1 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGCg                                                                                                             >  2:782783/1‑147 (MQ=255)                                          atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGGCCAATTCAAAAAAT‑GGGGTTGCCCTTATGAgggta                                                                                                           >  2:560299/1‑144 (MQ=255)                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAAAAAAAAA‑TGAGTTGTCATGATGAGCGAAAGgaa                                                                                                      >  1:71922/1‑149 (MQ=255)                                                 ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTCCAGACAAAAAAAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGgaataa                                                                                                   >  2:300034/1‑149 (MQ=255)                                                 ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTTATACAAAAAAAAAAAAT‑AGAGTTGGCATGTTGTGCGAAAGtaataa                                                                                                   >  1:222037/1‑149 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                              <  1:689472/150‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                            <  2:1011885/151‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                            <  1:231523/151‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACCAATAAAAAAACTAGGAG‑TGCCCTGATGAGCGAAAGTAATAAGCaa                                                                                               >  2:783539/1‑148 (MQ=255)                                                        tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                           <  1:817456/150‑1 (MQ=255)                                                          tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                         <  2:986772/150‑1 (MQ=255)                                                            gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAAAT‑GGAGGTGGCATGATGAGGGAAAGTAAAAAACaaaa                                                                                             >  2:159313/1‑142 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATACAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGTAATAAacaaca                                                                                             >  1:1069028/1‑140 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                   <  1:727135/150‑1 (MQ=255)                                                                 gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                 <  1:505711/151‑1 (MQ=255)                                                                   ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAaaaa                                                                                 >  2:849567/1‑149 (MQ=255)                                                                       cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAC‑TGCGATTACATGGTGCGCGAAAGAAATAAAAAAAAAGCAGGGAATAAGtt                                                                              >  1:749106/1‑148 (MQ=255)                                                                         aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                          <  1:776903/150‑1 (MQ=255)                                                                                 aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAT‑TGAGTTGCCATGATGAGGGAAAGAAATAAGAAACAGGCAGTGAATAAGATGACagag                                                                       >  2:457336/1‑145 (MQ=255)                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                 >  2:496253/1‑149 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCGACAAAAACAAAAACT‑GGAGTTGCCATGATGGGCGAAAGTAATAAAAAAAAAGCAGTGAATAAATTGACAGAGATTGTCg                                                                >  2:33922/1‑148 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                             <  1:1054258/151‑1 (MQ=255)                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAATAAGTTGACAGAGAtttttgc                                                               >  2:897225/1‑143 (MQ=255)                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACA‑GGAGATGCCCCGATGAGCGAAAGAAATAAACAACAAGCGGGGAATAAGGTGGCagcg                                                                       >  2:959559/1‑138 (MQ=255)                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAAAAAAAAAACT‑TGGGGTGCCATGATGAGCGGAAGAAATAAAAAAAAGGGCGGGAATAAATTGAGAGAGAATTTCGCt                                                              >  2:610312/1‑149 (MQ=255)                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAT‑GGAGGTGCCATGATGAGCGAAAGTAAAAAACAACAGGCAGGGGATAAGTTGAAAGAGATTGTCGCTaa                                                            >  2:1124999/1‑149 (MQ=255)                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACAAAAACT‑GGAGGTTCCATGAAGGGGGAAAGTAATAAGCAACATGCAGTGAATAAGTTGACAGAGAATGTCGCCaa                                                            >  2:1035337/1‑149 (MQ=255)                                                                                          tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAT‑GGAGGTTCCCTGATGAGGGAAAGGAATAAGAAACAAGGAGTGAATAAGTTTACAGAGAATGTCGCTAACttt                                                        >  1:1076884/1‑151 (MQ=255)                                                                                              ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                    <  1:423007/151‑1 (MQ=255)                                                                                               gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGc                                                   <  1:923114/151‑1 (MQ=255)                                                                                                  gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAAATACAAAAAAT‑GGGGGTGGCATGGTGAGCGAAAGTAATAAACAACAGGGAGTGAAAAAGTTTACAGAGATTTTCGCTAACTTTACCGcc                                                  >  1:1032545/1‑149 (MQ=255)                                                                                                      aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt                                            <  2:451143/151‑1 (MQ=255)                                                                                                      aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt                                            >  2:417829/1‑151 (MQ=255)                                                                                                      aaaaTATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATACAAAAACG‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGCCTCTAACTTTACCGCCATGAtt                                            <  1:931883/151‑1 (MQ=255)                                                                                                       aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAT‑GGAGGTGGCCTGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAATTGACAGAGATTGTCGCTAAATTTATCGCCATGAttt                                           >  2:738870/1‑151 (MQ=255)                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAAAACAAAAAAT‑GGAGTTGGCATGATGAGCGAAAGTAATAAACAAAAAGGAGGGGAAAAGGTGGCAGAGAATTGCGCTAACTTTACCGCCCTGAtttt                                          >  2:460859/1‑150 (MQ=255)                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCTAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTc                                          <  1:682826/151‑1 (MQ=255)                                                                                                          tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCCGACAAAAACAAAAAAT‑GGAGTTGCCATGATTAGCGGAAGCAATAAGGAAAAGGCAGGGAATAAATTGACAGAGATTGTCGCTAACTTTTCCGCCATGAttttt                                         >  2:137342/1‑148 (MQ=255)                                                                                                           ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAACAAAAAAT‑GGAGTTGCCATGATGGGCGAAAGGAATAAACAAAAGGCAGGGAATAAATTGGCAGAGATTGTCGCTAACTTTACCGCCATGAttttt                                         >  2:238116/1‑147 (MQ=255)                                                                                                           ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                       <  1:381188/151‑1 (MQ=255)                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACCAAAACAAAAAAT‑GGGGGTGGCATGATGAGCGAAAGGAATAAACAAAAAGCAGGGAATAAGTTGAAAGAGAATTTCGGTAAATTTAACGCCCAGATTTTTAACCGAATg                                >  1:433385/1‑151 (MQ=255)                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTTCGAAAAATACAAAAAAT‑GTGGTTTTCATGATTAGGGGAAGGAATAAAGAAAAGGGAGGGAATAAGTTTACAGAGATTGTCGCCCACTTTTACGCCATGATTTTTAccc                                     >  1:656602/1‑146 (MQ=255)                                                                                                                    caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGc                               <  2:457765/150‑1 (MQ=255)                                                                                                                     aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATATAAAAAAT‑GGGGGTGGCATGATGAGGGGAAAGAATAAAAAAAAGGAAGGGAAAAAGTTGAAAGAGATTGTCGCTAAATTTACCGCCCTGATTTTTACCCGAATGCCt                             >  1:1009581/1‑151 (MQ=255)                                                                                                                       ttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                            <  1:101953/150‑1 (MQ=255)                                                                                                                       ttttAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGGGGAAGTAATAAACAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGcc                              >  2:1114085/1‑148 (MQ=255)                                                                                                                        tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAAAAAAATCAAAAAACT‑GGAGTTGCCAGGAGGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCtg                            <  2:944446/149‑1 (MQ=255)                                                                                                                             tGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAAAAAAACT‑GGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                       <  2:766705/149‑1 (MQ=255)                                                                                                                                         gCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt         >  2:749839/1‑151 (MQ=255)                                                                                                                                            gTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa       >  1:1138306/1‑150 (MQ=255)                                                                                                                                               aaTTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAAT‑AGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa   <  1:690019/150‑1 (MQ=255)                                                                                                                                                 tttttCAAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg  <  1:750905/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAG  >  NC_000913/3206286‑3206579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑