Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,574,051 | 0 | G | T | 41.7% | 103.0 / 21.4 | 60 | V384V (GTC→GTA) | pqqL | putative periplasmic M16 family zinc metalloendopeptidase |
Reads supporting (aligned to +/- strand): ref base G (10/25); new base T (25/0); total (35/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.83e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG > NC_000913/1573902‑1574200 | aGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGa < 2:567941/151‑1 (MQ=255) gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGGTTTTAcatc > 1:597549/1‑151 (MQ=255) aaaGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGACATCATCg > 2:443988/1‑149 (MQ=255) aGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGtt < 2:936791/151‑1 (MQ=255) tGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGttct < 2:590896/150‑1 (MQ=255) tGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTACATCATCCAGgtctt > 1:292470/1‑151 (MQ=255) atGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagc < 1:673687/151‑1 (MQ=255) tctcTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACCTCCTTGGGGTCTTtagc > 1:505931/1‑148 (MQ=255) tctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGGAATTTACCTCATCGCGTTCTTTAGCAGaa > 2:900114/1‑150 (MQ=255) tCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGaaaa < 1:972112/149‑1 (MQ=255) ccGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTCCGCAGAAAAAcc > 1:596483/1‑151 (MQ=255) ccGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCCAGTTTTTTAGCAGaaaaa > 2:703388/1‑149 (MQ=255) cGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTTTTTCGCAGaaaaa > 2:753513/1‑148 (MQ=255) gACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATAGAGTTCTTCAGCAGAAAAAACACGc > 1:3385/1‑150 (MQ=255) gACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACAACATCGAGTTTTTTTGCAGAAAAAACATGCt > 2:113600/1‑151 (MQ=255) cAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTGCACAATCGCGTTCTTCAGCAGAAAAAACATggt > 2:568765/1‑147 (MQ=255) aaCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTTAGCAGAAAAAACAAGCTg > 2:767669/1‑146 (MQ=255) aaCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCaa < 2:55062/151‑1 (MQ=255) aCGGCGTATTATTTAATGAGCTGGATGCCAAGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACACCATCGAGTTTTTTTCCAGAAAAAACAAGCTGATCa > 2:855142/1‑149 (MQ=255) cGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTTTTTAGCAGAAAAAACATGCTGTTCaa > 1:447696/1‑149 (MQ=255) gCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGGTCTTTAGCAGAAAAACCATGCTGAt > 2:734717/1‑144 (MQ=255) gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTCAGCAGAAAAAACATGCTGATTAATGG‑tt > 1:754104/1‑150 (MQ=255) gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAAATTTGAAATCATCGAGGTTTTCTGCAGAAAAAACAAGCTGATCaa > 2:214888/1‑145 (MQ=255) ttAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAAct < 2:353419/150‑1 (MQ=255) ttAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTTTGCAGAAAAAACATGCTGATCAATGG‑TTGCCAAc > 2:508866/1‑149 (MQ=255) tAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTCTTCAGCAGAAAAAACAAGCTGATCAAAGG‑TTGCCaa > 1:384355/1‑147 (MQ=255) aaTGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGGTTTTACATCCACCAGGTTTTTCGCAGAAAAAACACGCTGATCAATGG‑GTGccca > 2:971949/1‑144 (MQ=255) gAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGTTTTTTAGCCGAAAAAACATGCTGATCAATGG‑TTGCCAACTCTGcc > 2:481216/1‑150 (MQ=255) ggATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTa < 2:18732/149‑1 (MQ=255) aGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTTCATCATCGAGGTTTTCAGCAGAAAAAACAAGCTGTTCAATGG‑TTTCCTACCCTTCCCTTAAtttatt > 2:1028267/1‑144 (MQ=255) cGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCgcag < 2:603297/151‑1 (MQ=255) cTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAGTTGACAACATCGAGGTCTTCAGCAGAAAAAACATGCTGATCAATTG‑TTGGCCAATCTGCCCTTTAAGCATTCccagc > 2:440821/1‑149 (MQ=255) tGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGGATTTTGAATCAATACGGTCTTTAAGAGAAAAAAAAAACTGATCAATGGTTTGCCAA‑TCTCCCCTTAAttccattccaac > 2:303009/1‑137 (MQ=255) tCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGGAATTTTACATCATCGAGTTTTTTAACAGAAAAACCAAGCTGGTCAAAGG‑TTGCCAAACCTGCCATTAATGCATTCCCAGCAccc > 2:606486/1‑149 (MQ=255) cAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTg < 2:607279/150‑1 (MQ=255) aGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCa < 1:468291/151‑1 (MQ=255) tAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCCAGGTCTTCCGCAGAAAAAACAAGCTGATTAATTG‑TTGCCCACTCTGCCATTAATGCATTCGCCGCATCCTGCAtatt > 2:153276/1‑147 (MQ=255) aCGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGt < 2:83839/151‑1 (MQ=255) cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg < 1:125045/151‑1 (MQ=255) cAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAtt < 2:690806/151‑1 (MQ=255) aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTTTTTTTCAGAAAAAACATGCTGATCAATTG‑TTGCCAACCCTTCCCTTAATTCATTTGCCGCAACCTGCATATTATCGTCTCCGGCAttt > 2:1017771/1‑151 (MQ=255) gCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTa < 1:665322/151‑1 (MQ=255) tGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTaca < 1:581251/150‑1 (MQ=255) tGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTaca < 1:440831/150‑1 (MQ=255) gCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACg < 2:434032/151‑1 (MQ=255) cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGCTAGATTTGGCATTATCCGGTTCTTCCGCAGAAAAAACACACTGATCAATCG‑GTGGCAACTCTGCCATTAATACATTTTCAACATCCTCCATAATTTCGtctccttaattt > 1:341274/1‑137 (MQ=255) aCCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACa < 1:5028/151‑1 (MQ=255) cATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGa < 1:214893/151‑1 (MQ=255) aTTTTTCAGCCAGGTGAGGCGGGGAGATTTTAAATCATCGAGGTTTTTTGCCGGAAAAACATGCTGGTTAATTG‑TTTGCAACTCTTCCCTTTATGCATTCGCAGCATCCTGCATATTATTTTCTCGGGCCTTTTCACGGgaaaaaa > 2:95554/1‑144 (MQ=255) ttttCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCCCGTTTTTTAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCCTTAATGCATTTGCCGCATCCTGCATATTATTCTCTCGTGCATTTACACGGGAAAAAAACGaat > 2:834538/1‑148 (MQ=255) tttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAGAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTg < 2:111475/150‑1 (MQ=255) aGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCggg < 2:940548/151‑1 (MQ=255) gCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAtt > 2:1006604/1‑151 (MQ=255) cGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAttt > 1:870005/1‑151 (MQ=255) ggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTa < 2:188392/151‑1 (MQ=255) ggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTa < 1:919224/151‑1 (MQ=255) tAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAAc > 2:516847/1‑151 (MQ=255) ttGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTg < 2:384346/150‑1 (MQ=255) tGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcg > 1:22786/1‑151 (MQ=255) tGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcg > 1:789502/1‑151 (MQ=255) | AGACGTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGG‑TTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG > NC_000913/1573902‑1574200 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |