Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	42.9%	69.2 / 29.2	56	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/27);  new base T (24/0);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 2.79e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206295‑3206584                                                                                                                                             |                                                                                                                                                       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                              <  2:621631/151‑1 (MQ=255)     aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACaaaaaaaa                                                                                                                                           >  2:287184/1‑145 (MQ=255)        cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAcaaaaacaaaaa                                                                                                                                        >  2:797568/1‑150 (MQ=255)         aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACaaaaa                                                                                                                                        >  1:107162/1‑149 (MQ=255)            gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                   <  1:966878/151‑1 (MQ=255)                 tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTg                                                                                                                              <  1:793252/150‑1 (MQ=255)                    cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GTTGGCat                                                                                                                          >  2:57032/1‑151 (MQ=255)                       ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAATACAAAAAATGGA‑GTTGCCatg                                                                                                                         >  2:226478/1‑149 (MQ=255)                           ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGA‑GGTGGCATGATGAg                                                                                                                    >  1:466648/1‑150 (MQ=255)                              gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GTTGGCATGATGAGCg                                                                                                                  >  1:325885/1‑149 (MQ=255)                               cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAACGGAGGTTGCC‑TGATGAGCgga                                                                                                                >  1:862138/1‑148 (MQ=255)                                       attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAg                                                                                                       <  1:862877/151‑1 (MQ=255)                                            tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                    <  2:912022/149‑1 (MQ=255)                                                 tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAAAAAAAAAAAAAGGA‑GTTGGCCTGATGGGGGaaaagaaaaagaaa                                                                                                    >  2:803710/1‑133 (MQ=255)                                                 tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                              <  2:633076/150‑1 (MQ=255)                                                    cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTCCAGACCAATAAAAAAAATGGG‑GGTGCCGTGATGAGCGGAAGGAATAAACAACAGGCAGt                                                                                            >  2:310730/1‑149 (MQ=255)                                                     gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                         <  2:96961/151‑1 (MQ=255)                                                        gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACTGGA‑GTTGCCATGATGAGCGAAAATAATAAACAACAAGccggggat                                                                                        >  1:58474/1‑142 (MQ=255)                                                            cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                   <  1:1005691/150‑1 (MQ=255)                                                               gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                               <  1:287855/151‑1 (MQ=255)                                                                 aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GTTGCCATGATGAGCGAAAGTAATAAACAAAAGGCAGTGAATAAATTGa                                                                                 >  1:318584/1‑147 (MQ=255)                                                                     ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGA‑GTTGCCATGATGAGCGAAAGGAATAAACAACAGGCAGGGAATAAATTGACAgagaat                                                                         >  1:313124/1‑149 (MQ=255)                                                                       aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTCCCGACAAATAAAAAAAATGGA‑GGTGCCATGATGAGCGAAAGAAAAAAAAAAAAAGGAGTGAAAAAAATGACAGAGAtt                                                                         >  1:457038/1‑149 (MQ=255)                                                                          tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCATGATGAGCGGAAGGAAAAAACAACAGGCAGGGAATAAGTTGACAGAGATTGTCg                                                                     >  1:25281/1‑150 (MQ=255)                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                                <  2:520709/150‑1 (MQ=255)                                                                                ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGA‑GTTGTCCTGGTGAGCGAAAAAAAAAAAAAACAGGAAGTGAATAAAATGACAGAGGTTTTCGCtca                                                                 >  2:913659/1‑146 (MQ=255)                                                                                ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGA‑GGTGGCATGATTAGCGGAAGTAAAAAACAAAAAGCAGGGAATAAATTGAAAGGGGTTGTGGGTaa                                                                 >  2:1016804/1‑148 (MQ=255)                                                                                 tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                              <  1:634671/150‑1 (MQ=255)                                                                                 tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                              <  1:803609/150‑1 (MQ=255)                                                                                 tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                              <  1:404987/150‑1 (MQ=255)                                                                                  cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGG‑GGTGCCATGATGAGGGGAAGGAATAAAAAACAGGCAGTGAATAAATTGGAAGAGATTGTCGCTAACttt                                                             >  1:892251/1‑150 (MQ=255)                                                                                  cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCATGATGAGCGAAAAAAATAAACAAAAGGCAGTGAATAAATTGACAGAGATTGTTGCTAACttt                                                             >  1:391/1‑150 (MQ=255)                                                                                   aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GGTGCCATGATGAGCGAAAGAAATAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCGCCAAAttt                                                             >  1:565006/1‑149 (MQ=255)                                                                                    cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGA‑GTTGCCCTGATGGGCGAAAGAAAAAAACAACAAGCAGGGAAAAAGTTGAAAGAGATTGTCGCTAACttt                                                             >  1:511099/1‑148 (MQ=255)                                                                                    cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                          <  2:888298/151‑1 (MQ=255)                                                                                     ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                         <  2:373396/151‑1 (MQ=255)                                                                                      gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGc                                                        <  1:541741/151‑1 (MQ=255)                                                                                          cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGA‑GGTGTCATGGTGAGTGGAAGTAAAAAACAAAAAGGAGTGAATAAAATGAGAGAGATTGTCGCTAACCTTTCCGcc                                                       >  2:377199/1‑148 (MQ=255)                                                                                           aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCATGATGAGCGAAAGAAATAAACAACAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc                                                       >  1:694567/1‑147 (MQ=255)                                                                                             aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAtt                                                 <  1:418233/151‑1 (MQ=255)                                                                                              aaaTATAAAACCACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGAttt                                                <  2:281924/151‑1 (MQ=255)                                                                                              aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAATACAAAAACTGGA‑GTTTCCCTGGTGAGGGGAAGTAATAAACAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                                >  2:615608/1‑151 (MQ=255)                                                                                                  ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAAATGGA‑GGTGGCATGATGAGCGAAAAGAAAAAACAAAAAGCAGTGAATAAATTGACAGAGATTTTCGCTAACTTTAACGCCAAGATTTCt                                              >  1:261897/1‑149 (MQ=255)                                                                                                     aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCg                                         <  1:659444/151‑1 (MQ=255)                                                                                                         cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                     <  2:512855/151‑1 (MQ=255)                                                                                                          acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGGCATGATGAGGGGAAGGAAAAAGCAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCCAATGc                                    >  2:361058/1‑151 (MQ=255)                                                                                                          acaTTTTTAGTGGTAGTTTGTGGCGGATAAATTTTCCCCGCAAATAAAAAAACTGGG‑GGTGTGCTGGGGGGCCTGAGGAATAAGCAACAGGAAATGAATAAGCTTAGAAAGATTGTTCCGATCTTTTCCGCCCAGCATTCTACCCgcaagc                                    >  2:237685/1‑146 (MQ=255)                                                                                                             tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAAAAAAAAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                                  <  2:824433/150‑1 (MQ=255)                                                                                                                          tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                     <  2:264593/150‑1 (MQ=255)                                                                                                                              tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  2:376448/149‑1 (MQ=255)                                                                                                                                gCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAGCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              <  2:107158/151‑1 (MQ=255)                                                                                                                                      aaTTTTTCCAGACAAATACAAAACCTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa          <  1:226484/149‑1 (MQ=255)                                                                                                                                      aaTTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa          <  2:958199/149‑1 (MQ=255)                                                                                                                                       aTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  1:593087/1‑149 (MQ=255)                                                                                                                                           ttCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa    <  1:514472/150‑1 (MQ=255)                                                                                                                                            tCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa    >  2:655573/1‑149 (MQ=255)                                                                                                                                             ccAGACAAATACAAAAACTGGA‑TTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  2:451294/150‑1 (MQ=255)                                                                                                                                             ccAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa   >  2:585401/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                       CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206295‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑