Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	50.0%	75.8 / 42.8	68	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (33/1);  new base C (0/34);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 2.46e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443127‑3443427                                                                                                                                                          |                                                                                                                                                       gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑a                                                                                                                                                        <  1:339759/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑Ac                                                                                                                                                       >  2:183349/1‑151 (MQ=255)        cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGc                                                                                                                                                 >  2:913570/1‑151 (MQ=255)         aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGcc                                                                                                                                                <  2:105218/151‑1 (MQ=255)            gaatAACACATAAAGCGGTTGCCCAGGCTAAAAATACAGCAAAATTGTTGCCACCCAGTT‑CCAACCAGTCCCCCCCCCGATCC‑ATGACGCGATGGTCGCCGGGAACAAAATAATACTGAAAGCAAAGATTGCCGGGATT‑CCCCCCGCCat                                                                                                                                              <  2:902365/147‑1 (MQ=255)             agTAACACAAAAAGCGTTGCCCCAGGCTGAAAATACAGCAAAATTGTTGTCAGCCAGTT‑CCAACCAGTCCCGCCCCCGAACC‑ATGACGCGTTGGTCGCGGGGAACAAAATAATACTGGAAGCAAAGATTGCCGGGATT‑CCCCCCGCCat                                                                                                                                              <  2:670319/149‑1 (MQ=255)                       aagggggTCGCCCGGGCTGAAAATACAGCAAATTTGTTGCCACCCAGTC‑CCACCCAGTCCCCCCCCCGACCA‑AGGACGCGGTGGCCGCCGGAAACAGAATAATCCTGGAAGCGAAGATGGCCGGGATT‑CCCCCCGCCATATTCACTTTCa                                                                                                                                   <  1:26345/145‑1 (MQ=255)                       aaaGCGGTGCCCCGGGCTAAAAAAACAGAAAAATTTTTGTCAGCCAGTC‑CCACCCAGTCCCCCCCCCAAACC‑AGGACGCGTTGGTCGCCGGAAACAAAATAATCCGGGAGGAGAAGATTGCCGGGATC‑CCCCCCGCCATATTCACTTTCAg                                                                                                                                  <  2:709749/151‑1 (MQ=255)                         aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCg                                                                                                                                >  1:246686/1‑151 (MQ=255)                          ggggTTGCCAAGGCGGAAAAAACAGAAAATTTGTTGTC‑GCCAATTCCCACCCAGCCCCCCCCCAGAACC‑ATGACGCGATGGTCGCGGGAAAAAAAATAATCCTGGAAGAGAAATTGGCCGGGATC‑CCCCCCGCCATATTCACTTTCAGCg                                                                                                                                <  2:825195/148‑1 (MQ=255)                            ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCgg                                                                                                                               >  1:427635/1‑149 (MQ=255)                            ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              >  1:689226/1‑150 (MQ=255)                              ttGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                            >  2:858118/1‑150 (MQ=255)                               tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                          >  1:727949/1‑151 (MQ=255)                                 cccggccTGAAAATACAGCAAATTTGTTTCCAGCCATTC‑CCAACCAGTCCCCCCCCCGAACC‑ATGACGCGATGGTCGCGGGAAACAAAATAATGCTGGAAGCGAAGTTTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                        <  1:974536/145‑1 (MQ=255)                                 cccGGGCTAAAAATAAAGAAAAATTTTGGTCAGCAAGTT‑CAAACCAGTCCCCCCCCAGAACC‑ATGACGAGTTGGTCGCGGGGAACAGAATAATATTAGAAGCGAAGTTTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                            <  1:411776/147‑1 (MQ=255)                                  ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       >  1:37350/1‑151 (MQ=255)                                     ggCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                    >  2:491745/1‑151 (MQ=255)                                     ggCTGAAAATACAGCAAAATTTTTGTCACCCA‑TT‑CCCACCCGGCCCCCCCCCCAACCAAGGCCGCGATGGCCGCCGGAAAAAAAATAATACTGAAAGCGAAAATTGCGGGAATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                     <  2:115650/150‑1 (MQ=255)                                       cTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  1:936244/1‑151 (MQ=255)                                       cTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  2:137738/1‑151 (MQ=255)                                        tGCAAATACAGAGAAATTGTGGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGAAGAGATGGTCGCCGGGAACAGAATAATAAGGGAAGCGAAGACAGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGAAAAtgtgtgaac                                                                                                                  >  2:744394/1‑147 (MQ=255)                                           aaaTACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                              >  2:918200/1‑151 (MQ=255)                                                  gCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCAGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:665395/1‑150 (MQ=255)                                                    aaaaTTGTTGTCAGCATGTC‑CCAACAAATCCCCCCCCCGACCA‑AGGGCGCGAGGGCCCCCGAAAAAAGAATAATACTGAAGGCGAAGATTGCCGGCTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                     <  2:171343/150‑1 (MQ=255)                                                     aaaTTGTTGTCAGCCATTC‑CCACCCAGTCCCGCCCCCGACCC‑ATGACGCGATGGTCGCCGGAAACAAAATAATCCTGGAAGAAAAGATTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                                                                       <  1:751538/148‑1 (MQ=255)                                                     aaaTTGTTGCCAGCAAGTC‑CCAACCAGTCCCGCCCCCGAACC‑AGGACGGGAGGGCCGCGGGGAACAAAATAATACTGGAAGCGAAGTTTGCGGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    <  1:100197/151‑1 (MQ=255)                                                       aTTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  >  1:75403/1‑151 (MQ=255)                                                        ttgtgtcccgactctt‑CCAACCTGACCCCCCCCCGAACC‑AAAATGCGTTGGTCGCGGGGAAAAGAAAAATATAGAAAGGAAACTTTGCGGGAATA‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  2:362282/135‑1 (MQ=255)                                                           ttgtCAGCCAGTC‑CCACCCAGTCCCCCCCCCGAACC‑ATGACGCGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGTTTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  1:480762/150‑1 (MQ=255)                                                           ttgtCAGCAATTC‑CCAACAAGTCCCCCCCCCGAACC‑ATGCGGCGAGGGTGGCGGGGAACAAAAAAATGTTGGAAGCAAAGTTTGCGGGGTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                                <  2:375341/149‑1 (MQ=255)                                                            ttgccggcaattcccACCCGGTCCCGCCCCCGACCC‑AGGACGCGAGGGGCGCGGAGAAAAAAATAATACGGGAGGCAAAGATTGCCGGGATC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  2:897144/137‑1 (MQ=255)                                                                  ccAGTT‑CCAACCAGTCCCGCCCCCGAACA‑ATGACGCGAGGGCCGCGGGAAAAAAAATAATCCTGGAAGGGAAGATTGCGGGTATC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                          <  1:34781/148‑1 (MQ=255)                                                                   cAGTC‑CCACCCAGTCCCCCCCCCGAACC‑ATGACGCGTTGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                      <  1:319535/151‑1 (MQ=255)                                                                         ccAACCAGTCCCCCCCCCAAACC‑AGGCCGCGGTGGCCGCGGGAAACAGAAAATTGCTGGAAGCAAAGATGGCCGGTATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  1:143315/150‑1 (MQ=255)                                                                         ccAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                                 >  1:697194/1‑151 (MQ=255)                                                                          ccaccgaggaccgccccGAACCAACGATGTGAGGGTGGCGGGAAACAAAATAATGCTGGAAGAG‑AGTTTTCCGGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  2:714676/137‑1 (MQ=255)                                                                           acccgttcccccccccGAACA‑AGGGCGCGATGGCCGCGGGAAAAAAAAAAATACTGGAAGGGAAGTTTGCGGGGTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  2:231874/137‑1 (MQ=255)                                                                             cctatccccccccccAACC‑ATGCCGCGAGGGGCGCGGGAAAAAAAAAAATACGGAAAGCAAAACTTGCGGGGTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               <  2:683343/140‑1 (MQ=255)                                                                             ccactcccccccccGAACC‑AGGGCGCGATGGTCGCCGGGAAAAAAATAATACTGGAGGCAAAAATTGCGGGTATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              <  2:257138/141‑1 (MQ=255)                                                                                 tACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                          >  2:185818/1‑150 (MQ=255)                                                                                  cccccccccAACCC‑ATGGCGCGATGGCCGCGGGAAACAAAAAAATACTGGAAGCGAAGATGGCCGGTATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                         <  2:393178/146‑1 (MQ=255)                                                                                   ccccccccGAACC‑ATGACGCGATGGTCGCGGGAAAAAAAAAAATACTGGAAGCAAAGATTGCGGGTTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       <  2:914253/148‑1 (MQ=255)                                                                                   ccccccccAACCC‑AGGACGCGATGGTCGCCGGAAAAAAAATAATACGGGAAGCAAAGATTGCGGGGTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                         <  1:419847/146‑1 (MQ=255)                                                                                     gCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     >  2:601308/1‑151 (MQ=255)                                                                                     ccccccGAACC‑ATGCCGCGATGGCCGCGGGAAAAAAAATAATACGGGAAGCAAAGTTTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     <  1:103659/150‑1 (MQ=255)                                                                                      cccccAACCC‑ATGCCGGGAGGGCCGCGGGAAAAAAAATAATCCGGGAAGCAAAGATTGCGGGTATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                    <  2:548522/151‑1 (MQ=255)                                                                                       ccccGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                   >  2:588938/1‑151 (MQ=255)                                                                                            aaCC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                               >  1:233297/1‑150 (MQ=255)                                                                                               c‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                           >  2:367905/1‑151 (MQ=255)                                                                                                 aggACGCGATGGCCGCCGGAAACAAAATAATCCTGGAGGCGAAGATGGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                          <  1:38078/149‑1 (MQ=255)                                                                                                           ggTCGCGGGAAAAAAAATAATCCGGGAAGAGAAGATTGCGGGTTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                                <  2:817054/151‑1 (MQ=255)                                                                                                            gTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                               >  2:372043/1‑151 (MQ=255)                                                                                                               gCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            >  2:983969/1‑151 (MQ=255)                                                                                                                cggggAAAAGAAAATTACGGGAAGCGAATTTTGCGGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  2:814850/148‑1 (MQ=255)                                                                                                                 cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           >  2:204367/1‑150 (MQ=255)                                                                                                                      aaaaaaaaataCTGGAAGCGAAGATTGCGGGGATC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                     <  2:381141/144‑1 (MQ=255)                                                                                                                          aaaaataCGGAAAGCGAAGTTGGCGGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 <  1:845429/148‑1 (MQ=255)                                                                                                                           ataataCTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 >  2:457511/1‑150 (MQ=255)                                                                                                                                aCTGGAAGCGAAGATTGCCGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                            <  1:650166/150‑1 (MQ=255)                                                                                                                                aCTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             >  2:547633/1‑149 (MQ=255)                                                                                                                                      aGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     >  1:314336/1‑151 (MQ=255)                                                                                                                                         gAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc                   >  2:685040/1‑150 (MQ=255)                                                                                                                                          aaGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                 >  2:63526/1‑151 (MQ=255)                                                                                                                                              ttGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              >  1:719725/1‑150 (MQ=255)                                                                                                                                              tggcggggTTT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  <  2:94118/141‑1 (MQ=255)                                                                                                                                                  cGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  1:496921/1‑151 (MQ=255)                                                                                                                                                    ggATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta        >  2:8776/1‑150 (MQ=255)                                                                                                                                                        t‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCATTGCAaatactaatac    >  2:992946/1‑150 (MQ=255)                                                                                                                                                          aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  1:704938/1‑151 (MQ=255)                                                                                                                                                          |                                                                                                                                                       GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTT‑CCAACCAGTACCGCCCCCGAACC‑ATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443127‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑