Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,556,207 | 0 | T | C | 42.1% | 79.7 / 20.1 | 57 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
Reads supporting (aligned to +/- strand): ref base T (28/5); new base C (0/24); total (28/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.85e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACC > NC_000913/3556057‑3556352 | gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑Ct > 1:903191/1‑151 (MQ=255) cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGc > 2:744031/1‑151 (MQ=255) aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAATAA‑CTCCCCGcc > 1:541696/1‑151 (MQ=255) ccaccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc > 2:653083/1‑149 (MQ=255) tCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt > 1:270923/1‑149 (MQ=255) gCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCg > 2:23857/1‑150 (MQ=255) cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGAt > 2:325413/1‑151 (MQ=255) aCTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGAtt > 1:963108/1‑151 (MQ=255) tgccgcgTTTTCCACCGACGAAAAAAAAGCGTTCGGTGATTTTTTCTCTTTCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAA‑CTCCCCGCCAGTGTAGCGAtt < 2:635829/146‑1 (MQ=255) tgccgcgCTTTTCCCCGACGAAAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATATTCTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGAtt < 1:871699/146‑1 (MQ=255) ccGACAAAAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTCAAAGCGAGACGGTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATAAATATTCTGTTCATGAAGGGAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa < 1:455922/148‑1 (MQ=255) cgaaaacAAAAAGGCGTTTGGTATTATTTCCACTTTGGACTAAAAGAGGCACGGTATTGCCCGTCCCTTGGTCGCGGGGACGGTTATTATTTTTCTCTACACGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg < 1:141459/146‑1 (MQ=255) acgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc > 1:980027/1‑151 (MQ=255) cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc > 2:390471/1‑150 (MQ=255) cgacAAAAAACGGTTGGTTAATTTTTTCCTTTCCGACTTAAAGGGAGCCGTTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa < 1:916575/146‑1 (MQ=255) gacAAAAATGCGTTCGCTGATTTTTCCCCTTTCGCCTCCAATAATACCGTTATGCCCCGCCCCCGGGTGGCCGGGCAGGTTATAAATTTTCTTTTCAAGGAGGAAAAAC‑CCCTCCGCCAGTGTAGCGATTTCACGCTCAGCAACa < 1:29154/145‑1 (MQ=255) acAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGCCTTCAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGGGGGAGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc < 2:796550/150‑1 (MQ=255) aaaaaggcttgggttGATATTTTCACTTTCGCCTACAGGCGAGAGGGTATTCCCCGGCCCGTGGTCGCGCGGCGGTTTATGAATATTTTGTTCATGCGGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg < 1:816972/135‑1 (MQ=255) aaaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc > 2:876401/1‑151 (MQ=255) aaaagGCTTTCGGTATTATTTCCCCTTCCGACTAAAAGGGAGCGGTTATCCCCCGCCCCCTGGCCGCGCGGGAGTTTATGAATTTTCTTTTCAGGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca < 2:9996/151‑1 (MQ=255) aaaaaGCTTTGGGTGATTTTTCCCCTTCCGACTCCAAGCGAGCCGTTTTTCCCCGCCCCCTGGCCGCGCGGAAGTTTATAAATTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca < 1:316050/151‑1 (MQ=255) ttCGGTGATTTTTCCCCTTCCGACTTCAGGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc < 1:657380/150‑1 (MQ=255) ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt > 1:848517/1‑151 (MQ=255) gggTAATATTTCCCCTTTCGACTCCAAGGGAGAGGGTATTCCCCGGCCCCGGGCCGGGCGGCAGGTTATGAATATTCTTTTCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa < 1:256908/150‑1 (MQ=255) ggTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt > 2:681596/1‑151 (MQ=255) tGATTTTTTCACTTCCGACTCAAAGCGAGACGTTTTTCCCCGGCCCCGGGGCGGGGGGCGGGTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa < 2:411343/150‑1 (MQ=255) tatTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc > 1:187152/1‑151 (MQ=255) atTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt > 1:30078/1‑151 (MQ=255) ttttCTTTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGCGGCGGTTTATAAATTTTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 1:480845/149‑1 (MQ=255) tttccccTTTCTCCTTCAAGCGAGCCGTTATCCCCCGCCCCTGGGCCGCGCGGCAGTTTATAAATTTTCT‑TTTATGAAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt < 2:486424/144‑1 (MQ=255) tttcCACTTCCGCCTAAAAGCGAGCGGTTATTCCCCGCCCCCGGGCCGCGCGGAAGTTTATAAATATTTTGTGCATGAAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa < 2:741849/147‑1 (MQ=255) tttCTCTTTCGTCTACAAGCGAGGCGTTATTACCGGCCCACTGGGCGCGGGGCAGGTTAAAAATATTTTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt < 2:67071/150‑1 (MQ=255) tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc > 2:10383/1‑151 (MQ=255) gACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc > 1:636036/1‑151 (MQ=255) ttCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc > 1:309059/1‑151 (MQ=255) tccAAGCGAGCCGTTTTTCCCCGGCCCCTGGTCGCGCGGCAGTTTAAGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa < 1:460191/144‑1 (MQ=255) aaGCGATACGTTATCCCCGGCCCCCTGCCCGCGGGGCAGTTTATAAATTTTTTGTCCAAGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg < 2:349105/141‑1 (MQ=255) gCGAGACGGTATCCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCAGGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca < 1:312659/149‑1 (MQ=255) agccgttATTCCCCGGCCCCTGGCCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt < 1:413758/144‑1 (MQ=255) ggTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg > 1:766276/1‑151 (MQ=255) tATTCCCCGCCCCGGGGTCGCGCGGCAGTTTATAAATATTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTcc < 1:532509/147‑1 (MQ=255) tATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgcg > 1:753025/1‑151 (MQ=255) aTTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCgcgc > 2:747817/1‑151 (MQ=255) tACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc > 2:90574/1‑151 (MQ=255) ccGCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc < 2:903168/151‑1 (MQ=255) cGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc > 2:507984/1‑150 (MQ=255) ccccTGGTCGCGCGGAAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGt > 1:615021/1‑151 (MQ=255) cccGGGTCGCGCGGCGGGTTAAAAATATTCTGTCCATGCAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt < 2:463660/151‑1 (MQ=255) cgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt > 1:269248/1‑150 (MQ=255) cgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt > 2:873700/1‑150 (MQ=255) cAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc > 1:706641/1‑151 (MQ=255) ttATGAATTTTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg < 1:217929/148‑1 (MQ=255) aTGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCCCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc > 2:628357/1‑151 (MQ=255) aaTATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg > 1:103935/1‑151 (MQ=255) tCTTTTCATCCGGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg < 2:850558/150‑1 (MQ=255) ggaaaaac‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGt < 2:194602/145‑1 (MQ=255) ggaaaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTg < 2:320469/147‑1 (MQ=255) aaac‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGccacc < 2:605846/151‑1 (MQ=255) | GTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACC > NC_000913/3556057‑3556352 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |