Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	41.5%	92.4 / 11.1	53	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (8/23);  new base G (22/0);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.43e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354150‑354437                                                                                                                                           |                                                                                                                                                       gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGagg                                                                                                                                                >  2:835260/1‑145 (MQ=255)   gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTg                                                                                                                                           >  2:743095/1‑150 (MQ=255)    aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑aa                                                                                                                                        <  2:649616/151‑1 (MQ=255)        tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAggg                                                                                                                                    >  1:590364/1‑151 (MQ=255)           cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCa                                                                                                                                  <  2:643148/150‑1 (MQ=255)                  cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAggtggcg                                                                                                                           <  2:358767/150‑1 (MQ=255)                      ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAggtggcggtg                                                                                                                        <  2:939084/149‑1 (MQ=255)                        aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGgtggcggtggcg                                                                                                                     <  1:301636/150‑1 (MQ=255)                        aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTG‑AAAAGGCAGggggcggtggcg                                                                                                                     >  2:924016/1‑150 (MQ=255)                         ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGCTGCGCTG‑GAAGGGCGGGGGGGGGGGGCGt                                                                                                                    >  2:889659/1‑150 (MQ=255)                         ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGATTCGGCG‑TAAGGGGTgggggcggcgggggt                                                                                                                   >  1:191414/1‑147 (MQ=255)                             cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTg                                                                                                                 <  1:928065/149‑1 (MQ=255)                             cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                                <  1:96382/150‑1 (MQ=255)                              gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCa                                                                                                              <  1:817674/151‑1 (MQ=255)                              gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTG‑GAAGGGGAGGGGGCGGGGGCGGTTGt                                                                                                                >  1:565210/1‑149 (MQ=255)                               ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCAt                                                                                                             <  1:970211/151‑1 (MQ=255)                                  gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                          <  1:251079/151‑1 (MQ=255)                                  gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                          <  2:137930/151‑1 (MQ=255)                                   cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGATGTGGTG‑AAAGGGGGGgggggggtggcg                                                                                                                     >  2:334738/1‑139 (MQ=255)                                    aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGGCG‑GAAGGGGGGGGGGCGGGGGGGGTTGGTATTc                                                                                                           >  2:537855/1‑148 (MQ=255)                                      tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGaa                                                                                                       >  2:922870/1‑150 (MQ=255)                                          gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGaaa                                                                                                      >  1:353090/1‑147 (MQ=255)                                          gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGGCG‑GAAGGGGAGGGGGGGGGGGGGTTTGtgcatccggaa                                                                                                      >  2:207032/1‑137 (MQ=255)                                            tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGTGGCGGTTGTCATTTCGAAagagag                                                                                                 >  1:990753/1‑150 (MQ=255)                                             gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                               <  1:69398/151‑1 (MQ=255)                                             gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGaaa                                                                                                      >  1:592401/1‑144 (MQ=255)                                              tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTG‑GAAGGGGAGGGGGGGGGGGGGGTTGTTTTTCCGGAaaagag                                                                                                 >  2:557075/1‑148 (MQ=255)                                                aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                            <  1:698472/151‑1 (MQ=255)                                                   aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTG‑AAAGGGGGGGGGGGGGGGGGGGTTGTTATTCCGGAAAAGGGCGGCa                                                                                            >  2:103563/1‑148 (MQ=255)                                                      gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGTGGAAAATGGGCCG‑AAAGGGGCGGTGGGGGGGGCGTTTGTGATTTCGCGAGAGCGGGACa                                                                                            >  2:269307/1‑145 (MQ=255)                                                        tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGGGGTTGTCATTCCGAAAGAGAGGGGCAGTCTgg                                                                                      >  1:838688/1‑149 (MQ=255)                                                              gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTG‑AAAGGGGAGGGGGCGGGGGCGGTTGTCCTTCCGAAAGAGAGGGACAGTCTgg                                                                                      >  1:147288/1‑143 (MQ=255)                                                               cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCCG‑GAAGGGGAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCg                                                                               >  2:869973/1‑149 (MQ=255)                                                                    gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGGAGGGGGGGGGGGGGGTTTTCATTTCGAAAAAGAGCGAAAGTTTGGGAGATCggg                                                                             >  2:718128/1‑144 (MQ=255)                                                                       ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  2:434078/151‑1 (MQ=255)                                                                               gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                             <  1:791202/151‑1 (MQ=255)                                                                                cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                            <  1:579210/151‑1 (MQ=255)                                                                                cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTG‑AAAAGGGAGGGGGCGGTGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                                 >  2:601436/1‑146 (MQ=255)                                                                                  agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                          <  2:575273/151‑1 (MQ=255)                                                                                    aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                           >  1:190445/1‑148 (MQ=255)                                                                                      tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        >  1:777566/1‑149 (MQ=255)                                                                                       gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  2:744902/150‑1 (MQ=255)                                                                                       gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAgg                                                     >  1:439856/1‑151 (MQ=255)                                                                                                       gCCATCCGGGCGTTGCCGAAGTGGCGGTTGTTTGGGGGAAAAAAGCGCTG‑AAAGGGGAGGGGGCGGGGGGGGTTGTCGTTCCGAAAAAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAAGCGAATATGGGGCtgg                                      >  2:680291/1‑150 (MQ=255)                                                                                                       gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  1:472424/151‑1 (MQ=255)                                                                                                        ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGTAAAGGGC‑GGGGGCGGCGGCGTTTTTCGTTCCGGAAGAGGGCGGCAGGCTGGGAGGTCGTGATGTGGCGCCACCGGCAGAGAAGGGGATTATGGCGCcgg                                      >  1:405508/1‑149 (MQ=255)                                                                                                                        gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCa                    <  2:636969/151‑1 (MQ=255)                                                                                                                                 gtggtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           <  1:331958/151‑1 (MQ=255)                                                                                                                                 gtggtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  2:443668/1‑151 (MQ=255)                                                                                                                                 gtggtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  1:756278/1‑151 (MQ=255)                                                                                                                                    gtTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc        >  1:178093/1‑151 (MQ=255)                                                                                                                                       ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg     <  1:689694/151‑1 (MQ=255)                                                                                                                                       ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      <  2:101795/150‑1 (MQ=255)                                                                                                                                           tGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  2:813445/1‑150 (MQ=255)                                                                                                                                           |                                                                                                                                                       GTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354150‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑