Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC52.6% 67.1 / 97.7 57T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (19/8);  new base C (0/30);  total (19/38)
Fisher's exact test for biased strand distribution p-value = 3.49e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-11

TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376358‑1376650
                                                                                                                                                    |                                                                                                                                                  
ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                 >  2:875613/1‑151 (MQ=255)
 tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                 >  2:96702/1‑150 (MQ=255)
 tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                >  1:863649/1‑151 (MQ=255)
  aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                 <  1:447695/149‑1 (MQ=255)
  aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                <  2:893050/150‑1 (MQ=255)
     gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                               <  2:660901/148‑1 (MQ=255)
      ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCg                                                                                                                                          <  1:711252/151‑1 (MQ=255)
      ccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCg                                                                                                                                          <  1:48304/151‑1 (MQ=255)
         aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATg                                                                                                                                       <  2:1076571/151‑1 (MQ=255)
          tGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCg                                                                                                                                          <  1:525643/147‑1 (MQ=255)
          tGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCg                                                                                                                                          >  2:525626/1‑147 (MQ=255)
             ccTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCccg                                                                                                                                   <  1:665215/151‑1 (MQ=255)
              cTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCccgc                                                                                                                                  >  2:379222/1‑151 (MQ=255)
              cTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCccgc                                                                                                                                  >  2:388039/1‑151 (MQ=255)
                 gccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCccgccg                                                                                                                                >  2:646370/1‑150 (MQ=255)
                             ggcgggCGACCAAAAAATGGGGGGTATTCCCCGGCGCTTCCGGAAACGGGCCGCCGCGCGTCCGGGAACC‑GTGGTGTATCTCGACCCTTTAACCCGCTATCAGAATCATATGACCACCCCCCCC‑GCCGATGCCCGCCGTTACAtcgtc                                                                                                                      <  2:660128/144‑1 (MQ=255)
                             ggACGGCGACCGAAAAAGGGGGAGTGTTCCCCTGCGCGTACTGGAACGGGTCGCCGCGCGTACCGGACCG‑GTGGTTTATCTCGACCCGTTAAACCGCTATCAGGATCATATGATCAACCCCCCC‑GCCGATGCCCGCCGTTACAtcgtc                                                                                                                      <  1:217924/148‑1 (MQ=255)
                                 ggCGCCCGAAAAATGGTGAGTGTTCCCCGGCGCGTACGGAAACGGGTCCCCGCGCGTCCCGGACCG‑GTGTTTTATCTCGACCCGTAAACCCCCTAGCGGAATAATATAAAAACCCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGaaaa                                                                                                                 <  2:824177/149‑1 (MQ=255)
                                 ggCGACCGAAAAAGGGTGAGTGATTCCCTGCGCGTACTGGAACAGGGCGCCGCGCGTCCCGAACCC‑GTGGTGTATCTCGAACCTTTAACCCGCTATCGGAATCATATGAACAACCCCCGC‑GCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                                <  1:222044/150‑1 (MQ=255)
                                 ggCGACCGAAAAAGGGTGAGTGATCCCCGGCGCGTACTGAAACAGGTCGCCGCGCGTCCGGGAACC‑GTGGTGTATCCCGACCCGTAAACCCGCTATAGGAATCATATGATCAACCCCCTC‑GCCGTTGCCCGCCGTTACATCGTCGAAAACg                                                                                                               <  1:780409/151‑1 (MQ=255)
                                 ggCGACCGAAAAAGGGGGAGTGATCCCCTGCGCGTACGGAAACAGGCCGCCGCGCGTCCCGGACCC‑GTGGTGTATCTCGACCCTTAAACCCGCTATCAGGATCATATGACCAACCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGaaaa                                                                                                                 <  1:271674/149‑1 (MQ=255)
                                      ccGAAAAATGGTGAGTGTTCCCCGGCGCTTATGGAAACGGGCCCCCGCGCGCCCCGAACCG‑GGGGTGTATCGCAACCCGTAAACCCGCTATCAGGATAAAATAACAACCCCCCCC‑GCCGTTCCCCGCCGTTACATCGTCGAAAAc                                                                                                                <  2:408973/145‑1 (MQ=255)
                                          aaaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                      >  2:787874/1‑151 (MQ=255)
                                                   agtgaTTCCCTGCGCGTACTGGAACAGTTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                >  2:875070/1‑148 (MQ=255)
                                                      gaTCCCCTGCGCTTACTGGAACAGGTCGCCGCGCGTCCCGGACCC‑GGGGTGTATCTCGAACCGTTAACCCGCTATCAGGATCATATAATAAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                          <  1:116566/151‑1 (MQ=255)
                                                        tccccTGCGCTTTCTGGAACAGGTCGCCGCGCTTACGGGACCC‑GTGGTTTATCCCGAACCTTTAAACCGCTATAGGAAATATATAACCAACCCCCTC‑GCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                              <  1:172321/143‑1 (MQ=255)
                                                           ccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                     >  1:1079277/1‑151 (MQ=255)
                                                               cgcgTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                                 >  1:869883/1‑151 (MQ=255)
                                                                       ggAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                         >  1:961249/1‑151 (MQ=255)
                                                                         aaCAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                       >  1:785572/1‑151 (MQ=255)
                                                                          aCAGGTCGCCGCGCGTACCGAAACC‑GTGGTGTATCTCGAACCGTAAAACCGCTATCAGAATCATATGACAAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcaca                                                                        <  1:875636/149‑1 (MQ=255)
                                                                                cgccgcGCGTACCGAACCC‑GTGGTGTATCTCGACCCGTTAACCCGCTATCAGGATAATATAACCAACCCCCCC‑GCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                  <  2:1087831/149‑1 (MQ=255)
                                                                                  cggcgcgTACCGGACCG‑GTGGTGTATCTCGACCCGTAAACCCGCTATCAGGATCATATGACAAACCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                               <  1:983465/148‑1 (MQ=255)
                                                                                  ccgcGCGTACCGGACCC‑GTGTTGTATCTCGACCCGTTAACCCGCTATCAGGATCATATGATCAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATc                                                                <  1:654843/149‑1 (MQ=255)
                                                                                  ccgcGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                               >  1:608958/1‑150 (MQ=255)
                                                                                  ccgcGCGTACCGGAACC‑GTGGTGTATCCCGACCCGTTAGACCGCTATCAGGATCATATGATCAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                              <  1:418931/151‑1 (MQ=255)
                                                                                       cgTACCGGACCC‑GTTTTGTATCTCGAACCGTTAACCCTCTATCAGGATAATTGTACCAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                           <  1:874687/149‑1 (MQ=255)
                                                                                        gcacggaaaCC‑GTGGTGTATCTCGAACCTTAAACCCTCTAGAAGAATCATATAATAAACCCCCTC‑CCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaaga                                                        <  2:873975/144‑1 (MQ=255)
                                                                                        gTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                           >  1:411408/1‑148 (MQ=255)
                                                                                        cgtcccgAACCGGTGGTTTTTCCCGACCCTTAAAACCGCTATAAGAATTATATGACCAACCCCCTC‑CCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                          <  2:461891/144‑1 (MQ=255)
                                                                                          aCCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                       >  1:355742/1‑150 (MQ=255)
                                                                                            cGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                                    >  1:1048924/1‑151 (MQ=255)
                                                                                             ggAACC‑GTGGTGTATCTCGACCCTTTAACCCGCTATAAGGATCATATGATAACCCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                   <  1:402923/151‑1 (MQ=255)
                                                                                             ggAACC‑GTGGTGTATCTCGAACCGTAAACCCGCTATCAGGATCATATGATCAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                   <  1:604673/151‑1 (MQ=255)
                                                                                                  cggtgttgTATCCCGAACCTTAAACCCGCTATAAGAATCATATGAACAACCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                               <  1:1179403/149‑1 (MQ=255)
                                                                                                    ggggttTATCGCGAGCTGTTAACCCGTTATCAGGATAATATAACCAACCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                <  2:833973/146‑1 (MQ=255)
                                                                                                       gtgtTTCTCGACCTGTAAACCAGCTATCAGAAAAATATGA‑CAACCCCCCCGGCTGATCACCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAgg                                          <  2:341990/151‑1 (MQ=255)
                                                                                                         gaaTCTCAAACCGTTAACCCGCTATAAGAATCATAAGATCACCCCCCTC‑GCCTGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                         <  1:947715/148‑1 (MQ=255)
                                                                                                               cGACCCGTAAACCCGCTATCAGAATCATATAAAAAACCCCCCC‑GCCTATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                  <  2:731381/151‑1 (MQ=255)
                                                                                                                 acccttaaaaCCGCTATAAGAATAATATAACCACCCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                        <  2:726400/136‑1 (MQ=255)
                                                                                                                      taaacccGTTATAAGGATCAATTGACCACCCCCCCC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAc                            <  2:1179318/145‑1 (MQ=255)
                                                                                                                        aacccGCTATCAGGATCATATAACCACCCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                         <  2:547905/148‑1 (MQ=255)
                                                                                                                          aCCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                        >  1:164801/1‑150 (MQ=255)
                                                                                                                           ccGCTAGCAGGAACATATAATCAACCCCCTC‑GCGGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAc                      <  1:191285/151‑1 (MQ=255)
                                                                                                                                ataagtaTCAAATAATAAACCCCCCC‑CCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCt                 <  2:1028766/145‑1 (MQ=255)
                                                                                                                                           atGATCAACCCCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt          <  1:1086472/147‑1 (MQ=255)
                                                                                                                                               tCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat  >  2:606533/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTC‑GCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAT  >  NC_000913/1376358‑1376650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑