Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,056	0	G	A	44.7%	92.7 / 39.9	76	intergenic (‑119/+84)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (31/11);  new base A (0/34);  total (31/45)
Fisher's exact test for biased strand distribution p-value = 2.36e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824907‑1825202                                                                                                                                                       |                                                                                                                                                   ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTg‑agaagaga                                                                                                                                                   <  1:990618/151‑1 (MQ=255)    aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGGGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGagaaa                                                                                                                                                 >  2:1091148/1‑150 (MQ=255)     cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGagaaa                                                                                                                                                 >  1:128947/1‑149 (MQ=255)      aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGagaaa                                                                                                                                                 >  2:1063395/1‑148 (MQ=255)           aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAAcc                                                                                                                                         <  1:941989/151‑1 (MQ=255)           aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAAcc                                                                                                                                         <  2:1168519/151‑1 (MQ=255)             gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAAccgc                                                                                                                                       <  1:47502/151‑1 (MQ=255)                 atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAAAAAACCGCCGa                                                                                                                                    <  2:768136/150‑1 (MQ=255)                     aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                               >  2:738891/1‑151 (MQ=255)                      aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                              >  1:330175/1‑151 (MQ=255)                            caatccTCGCTCCCGGCCGGTTTTTTTCTTTTTTATCTTTCAGGTACCCGTGTTTGTTTTCCGCCACCTGGTTTTTTTTACTTTAGTGGTGATCGTTTTTTTTTTCCACCTTTTTG‑AAAAAAAAAAAAAAAACCGCCGATCCTGTCCAcc                                                                                                                         <  2:917884/145‑1 (MQ=255)                             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                       >  2:371499/1‑151 (MQ=255)                             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                       >  1:991269/1‑151 (MQ=255)                                aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                    >  1:1001272/1‑151 (MQ=255)                                 cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGGGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                    >  1:361383/1‑150 (MQ=255)                                     cccccGGCCCTTTTTTTTCATTTTTATCGTTAAGGTAACCGGGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                <  1:473727/148‑1 (MQ=255)                                      tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                               >  2:317763/1‑150 (MQ=255)                                       cccGGCCCTTTTTTTTCATTTTTATCGTTCAGGTACCCGTGGATGTTTATCGGCAACTCGGGTAATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                                   <  2:471042/145‑1 (MQ=255)                                        ccGGCCCGTTCTTTTCATTTTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACACCTGTTGG‑AAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAg                                                                                                            <  1:881010/151‑1 (MQ=255)                                            cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                           >  2:306406/1‑148 (MQ=255)                                             ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                        >  2:1094703/1‑150 (MQ=255)                                               gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                     >  1:31664/1‑151 (MQ=255)                                                   ttttCTTTTTTATCTTTCAGGAACCCGTGGTGGTTTATCGGCACCTCGGTTATTTTCCGTTAGTGGTGATC‑GGGTTTTTTTTCAACTTTTTGAAAAAGAGAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                  <  2:956800/150‑1 (MQ=255)                                                   ttttCATTTTTATCTTTCAGGTACCCGTGGATGGTTATCGGCACCTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                  <  1:1042405/150‑1 (MQ=255)                                                   ttttCATTTTTATCTTTCAGGTACCCGTGGATGGTTATCGGCAACTCGGGTGTTTTACGTTAGTGGTGATCGGGTTTTTTTAACAACTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                   <  1:190141/149‑1 (MQ=255)                                                   ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                  >  2:1177439/1‑150 (MQ=255)                                                         ttGTTTTCTTTCAGGTAACGGGGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGTGGTGATCAGGCTTTTTTTAAATTTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                             <  1:1159610/149‑1 (MQ=255)                                                          ttttATCTTTCAGGTACCGGGGGTTGTTTATCGCCACCTGGGGTGTTTTAGGTTATTGGTATTCGGTTTTTTTTAACAATTTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                           <  1:474452/148‑1 (MQ=255)                                                           gTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                         >  1:1090595/1‑151 (MQ=255)                                                            ttatttttCAGGTAACGGTGTTTGTTTATGGGCAACGGGGGTATTTTACGTTAGGGGTGATCAGTTTTTTTTTACACCTGTTGG‑AAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                         <  2:106634/144‑1 (MQ=255)                                                            ttATCTTTCGGTTACCGGGGGATGTTTATCGAAAACTGGGGTGTTTTACTTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                          <  1:1092259/149‑1 (MQ=255)                                                            ttATCGTTCAGGTAACGGGGGTTGTTTATCGGCAACGGGGGTGTTTTATTTTAGTGGGTATCAGTTTTTTTTAACACTTGTTGG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                         <  2:905324/150‑1 (MQ=255)                                                             tatgtttCAGGTACCGGGGGATGTTTATCGGCAACTGGGGTGTTTTACGTTAGGGGTGATCGGTTTTTTTTTACACTTGTTGG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                        <  1:799067/145‑1 (MQ=255)                                                             tATTGTTCGGGTAACGGTGGTGGGTTAGCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGCTTTTTTTTACAACTGTTAG‑AAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                        <  1:353281/150‑1 (MQ=255)                                                                cgttaggttaacgttGGATGTTTTTCGTCACCTTGGGTAATTTGCGTATGGGGTGATCAGGTTTTTTTTAAAACTTTTTG‑AAAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                    <  1:483925/137‑1 (MQ=255)                                                                cGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                    >  1:249238/1‑151 (MQ=255)                                                                  ttCAGGTACCCGTGGATGGTTACCGGCAACTGGGTTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACACCTTTTGG‑AGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                   <  1:993478/150‑1 (MQ=255)                                                                    caggaaccggTGGATGTTTATCGCCAACTCGGTTGATTTCGGTTAGTGGTGACCGGGTTTTTTTTCCAACTGTTTG‑AGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                 <  2:1094333/141‑1 (MQ=255)                                                                      ggaaacgggggATGTTTAGCGGCAACGGGGGTGATTAACGTTGGTGGGGAGCAGTTTTTTTTAACAATTGTTGG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                                              <  2:915495/142‑1 (MQ=255)                                                                            cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGtttttt                                                                           >  1:6734/1‑148 (MQ=255)                                                                               ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt                                                                       >  2:795477/1‑149 (MQ=255)                                                                               ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                      >  1:284105/1‑150 (MQ=255)                                                                                gATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                      >  1:187286/1‑149 (MQ=255)                                                                                 aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGaa                                                                    >  2:110549/1‑150 (MQ=255)                                                                                  tGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                    >  1:8848/1‑149 (MQ=255)                                                                                   ggtttggggCAACGCGGGTGATTTACGTTAGTGGTGTTCAGTCTTTTTTTACAATTGTTTG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                                 <  2:963346/144‑1 (MQ=255)                                                                                     ttttCGCCAACTCGGGTTATTTACGTTAGGGGGGATCAGGCTTTTTTTAAAACTTTTTG‑AGAAGAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                <  1:537406/147‑1 (MQ=255)                                                                                     ttACCGGCACCTCGGGTGATTTACGTTAGTGGTGATCAGTTTTTTTTTACATCTTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                <  1:1145196/150‑1 (MQ=255)                                                                                     ttACCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACACCTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                    <  1:161673/146‑1 (MQ=255)                                                                                          ggaaaCTGGGGTGATTTACTTTAGTGGTAATCAGGTTTTTTTTACAACTGTTGG‑AGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                          <  1:1063422/148‑1 (MQ=255)                                                                                          ggCAACTGGGGTGTTTTAGGTTAGTGGTGTTCGGGTTTTTTTTACAATTGTTGG‑AGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                           <  2:1014339/150‑1 (MQ=255)                                                                                           gCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                            >  2:490916/1‑148 (MQ=255)                                                                                            cAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                        >  1:476589/1‑151 (MQ=255)                                                                                            cAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                         >  2:353179/1‑150 (MQ=255)                                                                                             acctgggttaaTTTACGTTAGTGGTGATCGGGTTTTTTTTACAATTGTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                         <  2:567763/139‑1 (MQ=255)                                                                                             aagtCGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTAACAGCTGTTAG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                          <  1:682792/145‑1 (MQ=255)                                                                                             aaCTGGGGTAATTTACTTTAGTGGTGATCAGGTTTTTTTAACAACTTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                       <  1:31781/151‑1 (MQ=255)                                                                                                  gggTGATTTCGGTGAGGGGGGACCGGTTTTTTTTTCCAAGTGTTGG‑AAAGAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                     <  2:804921/148‑1 (MQ=255)                                                                                                       ttttACGTTGGTGGTAATCAGGTTTTTTTTAAAATTTTTGG‑AAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                               <  2:121799/148‑1 (MQ=255)                                                                                                       aTTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                              >  1:495306/1‑150 (MQ=255)                                                                                                        tttagtttAGTGGTGATCAGTTTTTTTTTACACCTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                <  1:110700/141‑1 (MQ=255)                                                                                                        tttATGTTAGTGTTGATCGGGTTTTTTTTAAAATTTTTGG‑AAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                     <  2:979850/142‑1 (MQ=255)                                                                                                         ttATGTTAGTGGTGATCTGTTTTTTTTTACAACTTTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                            <  2:935136/150‑1 (MQ=255)                                                                                                            cGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGATGAAAGATTATTCAGCAGTTGCAGGCAtttt                                         >  2:542881/1‑150 (MQ=255)                                                                                                              ttggtggtaATCAGTTTTTTTTTACAATTTTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                        <  2:37879/146‑1 (MQ=255)                                                                                                              ttAGTGTTGATCAGGTTTTTTTAACAACTGTTTG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                        <  1:852963/149‑1 (MQ=255)                                                                                                              ttAGTGGTGATAAGGTTTTTTTTACAACTTTTTG‑AAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                        <  1:203471/149‑1 (MQ=255)                                                                                                                   ggtgATCAGGTTTTTTTTACAACTGTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                  <  1:662990/150‑1 (MQ=255)                                                                                                                   ggtaatcggtttttttttACACCTGTTTG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                     <  2:422387/136‑1 (MQ=255)                                                                                                                       aTCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                               >  2:256632/1‑149 (MQ=255)                                                                                                                                  tttACAATTGTTTA‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                   <  1:68930/150‑1 (MQ=255)                                                                                                                                   ttACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                 >  2:446371/1‑151 (MQ=255)                                                                                                                                   ttACAACTGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTg                  <  2:338200/150‑1 (MQ=255)                                                                                                                                    tACAACCGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGGTCTGt                 >  1:969700/1‑150 (MQ=255)                                                                                                                                     aCAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTg                  >  1:731968/1‑148 (MQ=255)                                                                                                                                      cAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCCGGACGTTCTGt                 >  2:791296/1‑148 (MQ=255)                                                                                                                                      cAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                >  2:650464/1‑149 (MQ=255)                                                                                                                                       agctttttG‑AGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg              <  2:31662/145‑1 (MQ=255)                                                                                                                                          tttttG‑AAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa               <  1:258141/144‑1 (MQ=255)                                                                                                                                          tGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc          >  1:579556/1‑151 (MQ=255)                                                                                                                                                 agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc     >  2:141838/1‑150 (MQ=255)                                                                                                                                                 agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc       >  1:886327/1‑148 (MQ=255)                                                                                                                                                 aaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc     <  2:256095/148‑1 (MQ=255)                                                                                                                                                    agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTTTGTCAGAAGCTTCTCaaa  >  2:950273/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824907‑1825202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑