Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,576,077	0	C	A	40.0%	101.4 / 30.9	60	D515Y (GAT→TAT)	yddB	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (29/7);  new base A (0/24);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 7.62e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACG  >  NC_000913/1575928‑1576213                                                                                                                                                         |                                                                                                                                         aCGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATc                                                                                                                                          >  2:898314/1‑150 (MQ=255) aCGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCa                                                                                                                                         <  1:236181/151‑1 (MQ=255)    cgcAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGa                                                                                                                                      <  2:485549/151‑1 (MQ=255)     gcAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGaa                                                                                                                                     <  1:976192/151‑1 (MQ=255)         tAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATAtt                                                                                                                                 >  1:866646/1‑151 (MQ=255)          aaCGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATAtt                                                                                                                                 <  2:769999/150‑1 (MQ=255)                cTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGcc                                                                                                                           >  2:880749/1‑150 (MQ=255)                  tACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCAt                                                                                                                         >  1:214140/1‑150 (MQ=255)                       aTTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAAc                                                                                                                   >  2:859245/1‑151 (MQ=255)                              gctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTtat                                                                                                             >  1:372598/1‑150 (MQ=255)                               ctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTtata                                                                                                            >  2:990526/1‑150 (MQ=255)                               ctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTtata                                                                                                            >  2:99742/1‑150 (MQ=255)                               ctgcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTtata                                                                                                            >  2:72388/1‑150 (MQ=255)                               ctccAACCCCATTCCTATTTAATCTTTATAGGGTTTTTAAAAA‑CCCTGATAGCGCTCCAGATTTTTTTTCCCTGATCCGGATCCCTT‑CCATCTTTGGGGAATACCACTAAACCCAAT‑AAAAAAAATCTTCCCGCCATAGTAACGGTtata                                                                                                            <  1:967697/147‑1 (MQ=255)                               ctccAACCCCATGGCCAGTTCATCGTTATAAGGTGTTTTAAAT‑TCCTGATAAGGCGTAAGTTTTTTATTACCTGAT‑CCCATTCCCTCCCACCTATGGGGAAAATCACGTAACCCAAT‑ACAAAAAATATTCCCGCCATAGTAACGGtt                                                                                                               <  1:335739/144‑1 (MQ=255)                                 gcAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAAcc                                                                                                         >  1:685282/1‑151 (MQ=255)                                   aaTCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACctg                                                                                                       >  2:621545/1‑151 (MQ=255)                                    aTCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACctgc                                                                                                      >  1:430665/1‑151 (MQ=255)                                      cccATTGCCAGTTTATCTTTATAAGGTTTTTAAAAA‑TCCTAATAACGCGTCAGATTTTTTTTACCTGATACCGTTTCCGC‑CCAGCTTTTGCGGATATCACGTAACCCCAT‑ATCAAAAATTTTCCCGCCATAGTAACGGTTATAACctgctg                                                                                                    <  2:604170/151‑1 (MQ=255)                                      cccATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACctgctg                                                                                                    >  1:194120/1‑151 (MQ=255)                                          ttGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAAt                                                                                                >  1:439894/1‑151 (MQ=255)                                           tGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATc                                                                                               >  1:688692/1‑151 (MQ=255)                                             ccAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCAt                                                                                             >  2:562882/1‑151 (MQ=255)                                              cAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCAtt                                                                                            >  2:730548/1‑151 (MQ=255)                                                 ttcttcTTTATAGGGCTTTTTAAAA‑TCTTGAAACCGCGTAAGATTTTTTTTCCCTGATCCCGTTCCCGT‑CCAGCTATGGGGGATATCCCTTAACCCCAA‑ATCAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGa                                                                                          <  2:176878/150‑1 (MQ=255)                                                 ttCCTCGTTATAAGGTGTTTACAAT‑CCCTGATAACGCGTAAAATTTTTTTTCCCTGAAACCGTTTCCGC‑CCGGCTTTCGCGAATACCACGTAATCCCAT‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGa                                                                                          <  2:459761/150‑1 (MQ=255)                                                 ttCATTTTTATAGGGCTTTTACAAT‑TCTTAATAACGCGTCAGTTTTTTTTTACCTGATACAGATCCCCT‑CCAGCTATGGCGGATATCACGTACCCCAAT‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGa                                                                                          <  1:385758/150‑1 (MQ=255)                                                 ttCATCTTTATAAGGCTTTTTAAAA‑TCTTGAAAACGCGTCAGATTTTTTTTCCCTGATCCCGATCCCTC‑CCATTTATGGCGGATACCACTTAACCCCAT‑ATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTg                                                                                           <  2:452930/149‑1 (MQ=255)                                                 ttCATCATTATCGGGC‑TTTTCAAACTCCTAAAAACGTATAAGATTTTTATTACCTGACCCAGATTCCTA‑CTAGTTATGGTGGTTAACAGGAAACCCAAC‑TTAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGa                                                                                          <  2:994699/150‑1 (MQ=255)                                                    aTCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGtt                                                                                      >  2:238751/1‑151 (MQ=255)                                                          aaagggCTTTTTCAAA‑TCGTGATACCGCGTCAGTTTTTTTTTCCCTGATCCCGATTCCTC‑CAAGTTATGGCGGATATCACGTAACCCAAT‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTg                                                                                    <  2:427065/143‑1 (MQ=255)                                                          aaagggCTTTTTCAAA‑TCCTGATACCGCGTCAGATTTTTATTCCCTGATCCCTATCCCGT‑CCAGCTTTTGCGAATATCACGTAACCCAAT‑ATCAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGAt                                                                                  <  2:248868/145‑1 (MQ=255)                                                            aaGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGc                                                                              >  2:726059/1‑151 (MQ=255)                                                                 gTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                          >  2:172856/1‑150 (MQ=255)                                                                  ttttCAAA‑GCCTAATAACGCGTCATTTTTTTATTCCTTGATACTTTTCCCTT‑CCAGCTTTTGCGGATATCACATAACCCAAT‑ATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                          <  1:1025221/149‑1 (MQ=255)                                                                  tttaaaaa‑CCCTGATAACGCGTAAGGTTTTTATTCCCTAATCCCATTCCCGT‑CCAGCTATTGGGAATACCACGTAACCCAAT‑AAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                          <  1:909989/144‑1 (MQ=255)                                                                     tacaattctttaTACCGCGTAAGATTTTTATTACCTAATCCCGATCCCGC‑CCATCTATTGGGAATTTATCTTAACCCAAT‑ATAAAAATTTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAata                                                                       <  1:731169/139‑1 (MQ=255)                                                                      cAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATcc                                                                    >  2:694590/1‑151 (MQ=255)                                                                        aa‑TCCTAATAACGCGTCAGATTTTTATTCCCTAATCCCGTTTCCGC‑CCAGCTATTGCGGATATCACTTAACCCCAT‑ATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCa                                                                  <  2:440262/151‑1 (MQ=255)                                                                                aTAACGCGTAAGATTTTTATTCCCTGATACCGATCCCGT‑CCAGCTATGGCGAATATCACGTACCCCAAT‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTc                                                           <  2:445183/151‑1 (MQ=255)                                                                                    cgcgTCAGTTTTTTTTTCCCTGATCCCTATCCCGC‑CCAGCTATTGCGAATATCCCGAACTCCCAT‑AAAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCAt                                                         <  1:654411/149‑1 (MQ=255)                                                                                      cgTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATaa                                                       >  1:110880/1‑149 (MQ=255)                                                                                              ttttttTCCCGGATCCCGATCCCGT‑CGAGTTTTGGCGGATACCGCTAACCCCCAT‑ACAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGcgcg                                                 <  2:93337/147‑1 (MQ=255)                                                                                              ttttATTCCCTGATCCCGATCCCGT‑CCAGCTTTTGCGAATATCACGTAACCCCAA‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGcgcg                                                 <  2:787638/147‑1 (MQ=255)                                                                                               ttttttCCCTGAACCTGATTCCGT‑CCAGTTATTGCGAATACCACGTACCCCCAT‑AAAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGga                                             <  1:650562/150‑1 (MQ=255)                                                                                                ttattaCCTAATCCCGATTCCGC‑CCAGTTATTGCGATTATCACGTAACCCCAT‑AAAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGgag                                            <  1:274155/150‑1 (MQ=255)                                                                                                  attaCCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGat                                          >  2:964001/1‑150 (MQ=255)                                                                                                   ttccgtGATCCCGTTTCCGC‑CCATCTATTGCGAATATCACGTAACCCAAT‑ACAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGat                                          <  2:23980/144‑1 (MQ=255)                                                                                                    taCCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATAtt                                       >  1:330642/1‑151 (MQ=255)                                                                                                        tGATGCCGATCCCGC‑CCAGCTATTGGGGTTAGCACGTAA‑CCCATAATAAAAATTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtgtg                                    <  2:624517/150‑1 (MQ=255)                                                                                                        tGATCCCGATTCCTC‑CCAGTTATTGGGAATATCACTTAATCCCAT‑AAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGata                                         <  1:898302/145‑1 (MQ=255)                                                                                                             ccGATTCCGC‑CCACTTTTTGCGGATATCACGAAATCCCAT‑AAAAAAAATATCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTgg                                   <  1:292923/146‑1 (MQ=255)                                                                                                                   ccGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGAt                         >  1:75374/1‑150 (MQ=255)                                                                                                                   ccGC‑CCAGCTTTTGCGGAATTCCCTTAACCCCAT‑AAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAg                           <  2:132098/148‑1 (MQ=255)                                                                                                                    cGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGAt                         >  1:717860/1‑149 (MQ=255)                                                                                                                     cgccaaattgttGGGGATATCAGGAATTCCTAT‑ATAAAGAATGTGCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATa                        <  2:570754/140‑1 (MQ=255)                                                                                                                      t‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGt                      >  1:221761/1‑150 (MQ=255)                                                                                                                        ccAGTTATTGCGGATATCACTTAACCCCAT‑ATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGt                      <  1:2268/149‑1 (MQ=255)                                                                                                                                tGCGGATATCACGTACTCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCCTTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcatagtc            >  1:125773/1‑151 (MQ=255)                                                                                                                                          aCGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg  >  1:505671/1‑151 (MQ=255)                                                                                                                                          aCGTAATCCCAT‑ATCAAGAATATTACCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGTGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGCGATATTGTGGTTTGACAGCTAGTTGTCATAGTCATACCGCAc   >  1:323130/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                         ACGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAA‑TCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGT‑CCAGCTATTGCGGATATCACGTAATCCCAT‑ATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACG  >  NC_000913/1575928‑1576213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑