Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,177,748 | 0 | C | A | 41.4% | 80.5 / 30.7 | 58 | E55* (GAG→TAG) | nudF | ADP‑ribose pyrophosphatase |
Reads supporting (aligned to +/- strand): ref base C (30/4); new base A (0/24); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.60e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGG > NC_000913/3177601‑3177893 | tttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCaa > 2:882620/1‑150 (MQ=255) ttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCaa < 1:436955/149‑1 (MQ=255) aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt > 2:327022/1‑151 (MQ=255) tCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc > 2:995628/1‑151 (MQ=255) atcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAcc > 1:337624/1‑150 (MQ=255) cGGGAACCATATCCCGTAGCCAAGGGGGTTCGCTGGGTTCTTACGCCGCCACCCGAATCTGCTCAATCGGCACAAATCAAACACGCGCTTGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCCCCCTCAAAAATTTCCCGCCCCCCCTc > 1:611876/1‑148 (MQ=255) cAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACt > 1:916967/1‑151 (MQ=255) aGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt > 2:686243/1‑151 (MQ=255) gTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt < 2:410614/151‑1 (MQ=255) gCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGa > 1:522333/1‑150 (MQ=255) ccAGGGGGTTGCGCGGGTGCCGTCCGCGGCAACCCGACTCTGCTCAACCAGCACACCTACATCACCCACTGGGTCAAAGGGT‑AGCAAGCCTGCGGCGTGACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGtt < 1:282717/147‑1 (MQ=255) tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACGGGGTAAAAGGGT‑AGCAAGCCTGGGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt < 2:754207/150‑1 (MQ=255) ttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg > 2:95953/1‑150 (MQ=255) tccGCTGGTGTCAGACGCGGAAATCCGAATCTGCTCAATCAGCACAACTTCACCCCCCACTGGGTCAAGGGGT‑AGCAAGACTGCGGCTTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg < 1:149946/148‑1 (MQ=255) tCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGa < 1:464520/150‑1 (MQ=255) tGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa > 2:247130/1‑150 (MQ=255) ggTGTCGTACGCGGAAATCCGAATCTGCTCAACCAGCACAACTTCATCACGCACGGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa < 1:874550/150‑1 (MQ=255) gtccgcgGCAATCCAAATCTCCACAATCAGCACAACTTCATCCCCCACTGGTTCAAGGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa < 2:860756/148‑1 (MQ=255) cgcgGCAATCCGAATCTGCTCAATCAGCACAACTGCACCACGCACTGGGTCAAAGGGT‑AGCAAGCCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtat < 1:304273/146‑1 (MQ=255) cgcgGCAATCCGAATCTGCTCAAACAGCACAACTTCACCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAg < 1:246128/150‑1 (MQ=255) cAACCCGACCCTGCTCAATCACCACAACTTCATCCCGCA‑TTGGTTAAAGGTTCCAAAAGTCGGCGGCGTGCCCCCGGCAAAAAATCACCCGCCGCACCTCATGACGCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa < 1:572392/143‑1 (MQ=255) aTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg > 2:665338/1‑151 (MQ=255) gAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa > 1:615137/1‑150 (MQ=255) gAATCTGCTCAACCAGCACACCTCCACCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg < 1:338154/147‑1 (MQ=255) aatttccTCAATCAGCACAACTTCATCACCCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa < 2:881828/145‑1 (MQ=255) tGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGc > 2:174954/1‑151 (MQ=255) tGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGAAGATGACGAAATCTATAAAGATCTAATGAAAAAAAg > 1:333317/1‑150 (MQ=255) gCTCAATCAGCACAACTTCACCACGCACTGGGTAAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAg < 2:877062/149‑1 (MQ=255) cccAATCAGCACAACTTCACCCCGCACTGGGTAAAGGGGT‑AGCAAGACCGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg < 2:610139/149‑1 (MQ=255) cccAACCAGCACATCTTCATCCCCCACTGGGTAAAAGGGT‑AGCAAGACTGCGGCGTGCCCCGGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaaa < 1:644921/145‑1 (MQ=255) cTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg > 1:306339/1‑151 (MQ=255) tCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgc > 1:222432/1‑151 (MQ=255) tCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt > 2:764941/1‑150 (MQ=255) cAGCACAACTTCATCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt < 2:520835/149‑1 (MQ=255) aGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaa > 2:804468/1‑150 (MQ=255) cacaACTTCACCCCGCACGGGGTCAAAGGGT‑AGAAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaaa < 1:217564/149‑1 (MQ=255) acaACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg > 1:554654/1‑151 (MQ=255) caACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtgt > 1:626084/1‑151 (MQ=255) aCTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGttt > 1:108012/1‑151 (MQ=255) tcaCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg > 2:259346/1‑151 (MQ=255) aCTGGGTCAAAGGGT‑AGCAAGACTGCGGCTTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa < 2:782170/151‑1 (MQ=255) aCTGGGTAAAGGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa < 1:798552/151‑1 (MQ=255) gggTCAAAGGGT‑AGCAAGACTGCGGGGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa < 2:380322/148‑1 (MQ=255) gggTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAttt > 1:298292/1‑151 (MQ=255) aaaGGGT‑AGCAAGCCTGCGGCGTGACCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACa < 2:207438/151‑1 (MQ=255) aaGGGT‑AGAAGGCCTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt < 2:559259/151‑1 (MQ=255) gggT‑AGCAAGATTGCGGAGTGACCGCATTAAAAAATTCACCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAACAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCg < 1:189379/151‑1 (MQ=255) ggT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt > 2:131733/1‑151 (MQ=255) t‑AGAAAGACGGCGGCGGGCCCCCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtt < 1:851186/150‑1 (MQ=255) gCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt > 1:971262/1‑151 (MQ=255) aaGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTATACATCGTTTTTGc > 2:874378/1‑151 (MQ=255) aaGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt < 1:887210/149‑1 (MQ=255) aggcTGCGGCGTAACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGcc < 2:638133/148‑1 (MQ=255) tGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt > 1:372085/1‑151 (MQ=255) tGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt > 2:736385/1‑151 (MQ=255) gcggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt > 2:381475/1‑150 (MQ=255) ggcgTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTa < 2:234303/151‑1 (MQ=255) gcgcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAgg > 2:152261/1‑151 (MQ=255) | TTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGG > NC_000913/3177601‑3177893 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |