Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2000GA48.2% 47.2 / 35.1 56S217F (TCC→TTC) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (23/6);  new base A (0/27);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 1.56e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTT  >  NC_000913/3556051‑3556341
                                                                                                                                                       |                                                                                                                                             
ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAggg                                                                                                                                               <  1:877157/150‑1 (MQ=255)
ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAggg                                                                                                                                               <  1:253656/150‑1 (MQ=255)
      gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                        <  1:677640/151‑1 (MQ=255)
        gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcc                                                                                                                                      <  1:744470/151‑1 (MQ=255)
            cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGc                                                                                                                                  <  1:159013/151‑1 (MQ=255)
             aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                 >  2:284919/1‑151 (MQ=255)
                                cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                              >  2:973214/1‑151 (MQ=255)
                                cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                              >  2:937789/1‑151 (MQ=255)
                                    tttCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                          >  1:547348/1‑151 (MQ=255)
                                      tccccaccgaaAAAAAGCCGTTGGGTCATATTTTCCCGTCCGACTCCAAGCGAGCCGTTATTACCCGCCCCCGGGCCGCGCGGCGGTTTATGAATTTTCGTTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                          <  1:761265/142‑1 (MQ=255)
                                       cACCGACAAAAAAAAAGCTTTGGGTAATATTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCCGGGCCGCGCGGCAGTTTATAAATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa                                                                                                        <  2:358814/150‑1 (MQ=255)
                                             ccaaaaaaaaCCTTTGGGGGATATTTTCCCTTCCGCCTCAAGGCGAAGCGGTTTTCCCCGGCCCGTGGTGCCGGGGCAGGTTAGAATTTTTTGGTGCATGCGGAGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                   <  1:684083/145‑1 (MQ=255)
                                                cAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                              >  1:1019160/1‑151 (MQ=255)
                                                  aaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                             >  1:905169/1‑150 (MQ=255)
                                                       gCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                        >  2:898057/1‑150 (MQ=255)
                                                        cGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                        >  2:99538/1‑149 (MQ=255)
                                                         gTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                     >  1:651304/1‑151 (MQ=255)
                                                          ttgggTAATATTTTCACTTTCGCCTCCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGGGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg                                                                                      <  2:635694/146‑1 (MQ=255)
                                                          ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                    >  2:303602/1‑151 (MQ=255)
                                                            cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                   >  2:897420/1‑150 (MQ=255)
                                                             gttGATATTTTCTCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                   <  2:773286/147‑1 (MQ=255)
                                                             ggTGATTTTTTCCCTTCCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                     <  2:13122/147‑1 (MQ=255)
                                                              gtatttttttCACTTTCGACTTCAGGCAAGCCGGTTTCCCCCGGCCCCGGGTCGCGCGGAGGGTTATAAATATTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                  <  2:869424/143‑1 (MQ=255)
                                                                  ttttttCTCTTTCGCCTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                               <  2:888057/146‑1 (MQ=255)
                                                                  ttttttCACTTGCGACTACAAGCGAGACGGTATTCCCCGCCCCCGGGCCGCGGGGCGGGTTATAAATATTCTTTTCAGGGAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                              <  1:238735/147‑1 (MQ=255)
                                                                    ttttCCCTTTCGACTACAAGCGAGACGTTATCCCCCGGCCCCGGGCCGCGCGGCGGGTTATAAATATTTTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                            <  2:603108/149‑1 (MQ=255)
                                                                    ttttCACTTTCGACTCCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                            <  2:518827/149‑1 (MQ=255)
                                                                     tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                         >  2:512200/1‑151 (MQ=255)
                                                                      ttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                        >  2:350965/1‑151 (MQ=255)
                                                                           tttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                   >  2:562181/1‑151 (MQ=255)
                                                                            ttCGTCTACAAGCGAGTCGTTTTTCCCCGCCCCCTGGCCGCGCGGGAGGTTATGAATTTTCTTTTAATGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                   <  1:831033/150‑1 (MQ=255)
                                                                              cGACTTCAAGCGAGACGTTATCCCCGGCCCCTTGGTCGCGCGGCAGTTTATAATTTTTTTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                   <  1:797829/148‑1 (MQ=255)
                                                                              cGACTCCAAGCGAGACGGTATTCCCCGCCCCCGGGCCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                  <  2:510241/149‑1 (MQ=255)
                                                                                    cAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                          >  1:101399/1‑151 (MQ=255)
                                                                                     aaGCGAGACGGTATTCCCCGCCCCGTGGGCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                         <  1:167796/151‑1 (MQ=255)
                                                                                         gagaCGTTATTCCCCGCCCCCTGTCCGCGCGGCAGTTTATGAATTTTTTTTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                       <  1:445317/149‑1 (MQ=255)
                                                                                         gagaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                     >  2:464192/1‑151 (MQ=255)
                                                                                         gagaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                     >  1:653045/1‑151 (MQ=255)
                                                                                                tATTCCCCGCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                <  1:336425/149‑1 (MQ=255)
                                                                                                   tACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                           >  1:538304/1‑151 (MQ=255)
                                                                                                    tcccggcacccggcgcgcgcgGGCGGTTATTACTTTTCTGTTCAGGAAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt                                            <  2:72982/135‑1 (MQ=255)
                                                                                                    ccccGCCCCCTGGTCGCGGGGCATTTTATAAATTTTCTTTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                          <  1:1024485/150‑1 (MQ=255)
                                                                                                     cccGGCCCCTGGTCGCGGGGCAGGTTTTGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                           <  1:299739/149‑1 (MQ=255)
                                                                                                     cccGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                         >  2:1007204/1‑151 (MQ=255)
                                                                                                     cccGGCCCCTGGGCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                           <  1:978486/149‑1 (MQ=255)
                                                                                                          ccccGGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCa                                       <  1:388357/148‑1 (MQ=255)
                                                                                                             cTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                 >  1:552778/1‑151 (MQ=255)
                                                                                                                   cgggcgcaagtttATGAATTTTCTTTTCGTGCAGAAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCaa                                      <  1:31664/130‑1 (MQ=255)
                                                                                                                    gcgcgGCAGTTTATAAATTTCCTGTCCATGGGGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgt                            <  1:62794/150‑1 (MQ=255)
                                                                                                                    gcgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                           >  1:130083/1‑151 (MQ=255)
                                                                                                                          cAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                     >  1:812972/1‑151 (MQ=255)
                                                                                                                              ttATGAATATTCTGTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                  <  2:424323/150‑1 (MQ=255)
                                                                                                                                  aaaTATTTTGTGCATAAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                   <  1:973193/144‑1 (MQ=255)
                                                                                                                                     tgttCTTTCCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg           <  1:169537/148‑1 (MQ=255)
                                                                                                                                       ttCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc         <  2:131143/150‑1 (MQ=255)
                                                                                                                                             ttCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag      <  2:1015783/148‑1 (MQ=255)
                                                                                                                                              tCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt  >  1:474360/1‑151 (MQ=255)
                                                                                                                                                 ggCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg        <  1:354114/141‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                             
CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTT  >  NC_000913/3556051‑3556341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: