Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,556,200 | 0 | G | A | 48.2% | 47.2 / 35.1 | 56 | S217F (TCC→TTC) | rtcA | RNA 3'‑terminal phosphate cyclase |
Reads supporting (aligned to +/- strand): ref base G (23/6); new base A (0/27); total (23/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.56e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTT > NC_000913/3556051‑3556341 | ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAggg < 1:877157/150‑1 (MQ=255) ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAggg < 1:253656/150‑1 (MQ=255) gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACt < 1:677640/151‑1 (MQ=255) gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTcc < 1:744470/151‑1 (MQ=255) cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGc < 1:159013/151‑1 (MQ=255) aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGcc > 2:284919/1‑151 (MQ=255) cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt > 2:973214/1‑151 (MQ=255) cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt > 2:937789/1‑151 (MQ=255) tttCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc > 1:547348/1‑151 (MQ=255) tccccaccgaaAAAAAGCCGTTGGGTCATATTTTCCCGTCCGACTCCAAGCGAGCCGTTATTACCCGCCCCC‑GGGCCGCGCGGCGGTTTATGAATTTTCG‑TTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc < 1:761265/142‑1 (MQ=255) cACCGACAAAAAAAAAGCTTTGGGTAATATTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCC‑GGGCCGCGCGGCAGTTTATAAATTTTCT‑TTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa < 2:358814/150‑1 (MQ=255) ccaaaaaaaaCCTTTGGGGGATATTTTCCCTTCCGCCTCAAGGCGAAGCGGTTTTCCCCGGCCCG‑TGGTGCCGGGGCAGGTTA‑GAATTTTTTGGTGCATGCGGAGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg < 1:684083/145‑1 (MQ=255) cAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg > 1:1019160/1‑151 (MQ=255) aaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc > 1:905169/1‑150 (MQ=255) gCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc > 2:898057/1‑150 (MQ=255) cGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc > 2:99538/1‑149 (MQ=255) gTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc > 1:651304/1‑151 (MQ=255) ttgggTAATATTTTCACTTTCGCCTCCAAGCGAGACGTTATTCCCCGGCCCC‑TGGTCGCGGGGCAGTTTATGAATATTCT‑GTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg < 2:635694/146‑1 (MQ=255) ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt > 2:303602/1‑151 (MQ=255) cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa > 2:897420/1‑150 (MQ=255) gttGATATTTTCTCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCC‑GGGTCGCGCGGCAGTTTATGAATATTCT‑GTTCATGCGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa < 2:773286/147‑1 (MQ=255) ggTGATTTTTTCCCTTCCGACTTCAAGCGAGACGGTATTCCCCGGCCCC‑TGGTCGCGCGGCAGTTTATGAATTTTCT‑GTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc < 2:13122/147‑1 (MQ=255) gtatttttttCACTTTCGACTTCAGGCAAGCCGGTTTCCCCCGGCCCC‑GGGTCGCGCGGAGGGTTATAAATATTCT‑GTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa < 2:869424/143‑1 (MQ=255) ttttttCTCTTTCGCCTTCAAGCGAGACGTTATTCCCCGGCCCC‑TGGTCGCGCGGCAGTTTATGAATTTTCT‑GTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg < 2:888057/146‑1 (MQ=255) ttttttCACTTGCGACTACAAGCGAGACGGTATTCCCCGCCCCC‑GGGCCGCGGGGCGGGTTATAAATATTCT‑TTTCAGGGAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa < 1:238735/147‑1 (MQ=255) ttttCCCTTTCGACTACAAGCGAGACGTTATCCCCCGGCCCC‑GGGCCGCGCGGCGGGTTATAAATATTTT‑TTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 2:603108/149‑1 (MQ=255) ttttCACTTTCGACTCCAAGCGAGACGGTATTCCCCGGCCCC‑TGGTCGCGCGGCAGTTTATGAATATTCT‑GTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 2:518827/149‑1 (MQ=255) tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc > 2:512200/1‑151 (MQ=255) ttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct > 2:350965/1‑151 (MQ=255) tttCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg > 2:562181/1‑151 (MQ=255) ttCGTCTACAAGCGAGTCGTTTTTCCCCGCCCCC‑TGGCCGCGCGGGAGGTTATGAATTTTCT‑TTTAATGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg < 1:831033/150‑1 (MQ=255) cGACTTCAAGCGAGACGTTA‑TCCCCGGCCCCTTGGTCGCGCGGCAGTTTATAATTTTTTT‑TTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg < 1:797829/148‑1 (MQ=255) cGACTCCAAGCGAGACGGTATTCCCCGCCCCC‑GGGCCGCGCGGCAGGTTATGAATTTTCT‑GTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc < 2:510241/149‑1 (MQ=255) cAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac > 1:101399/1‑151 (MQ=255) aaGCGAGACGGTATTCCCCGCCCCG‑TGGGCGCGCGGCAGTTTATGAATATTCT‑GTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca < 1:167796/151‑1 (MQ=255) gagaCGTTATTCCCCGCCCCC‑TGTCCGCGCGGCAGTTTATGAATTTTTT‑TTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt < 1:445317/149‑1 (MQ=255) gagaCGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt > 2:464192/1‑151 (MQ=255) gagaCGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt > 1:653045/1‑151 (MQ=255) tATTCCCCGCCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg < 1:336425/149‑1 (MQ=255) tACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc > 1:538304/1‑151 (MQ=255) tcccggcacccggcgcgcgcgGGCGGTTATTACTTTTCT‑GTTCAGGAAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt < 2:72982/135‑1 (MQ=255) ccccGCCCCC‑TGGTCGCGGGGCATTTTATAAATTTTCT‑TTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg < 1:1024485/150‑1 (MQ=255) cccGGCCCC‑TGGTCGCGGGGCAGGTTTTGAATTTTCT‑GTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc < 1:299739/149‑1 (MQ=255) cccGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc > 2:1007204/1‑151 (MQ=255) cccGGCCCC‑TGGGCGCGCGGCAGTTTATGAATATTCT‑GTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc < 1:978486/149‑1 (MQ=255) cccc‑GGGTCGCGCGGCAGTTTATGAATATTCT‑GTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCa < 1:388357/148‑1 (MQ=255) c‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc > 1:552778/1‑151 (MQ=255) cgggcgcaagtttATGAATTTTCT‑TTTCGTGCAGAAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCaa < 1:31664/130‑1 (MQ=255) gcgcgGCAGTTTATAAATTTCCT‑GTCCATGGGGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgt < 1:62794/150‑1 (MQ=255) gcgcgGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg > 1:130083/1‑151 (MQ=255) cAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc > 1:812972/1‑151 (MQ=255) ttATGAATATTCT‑GTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt < 2:424323/150‑1 (MQ=255) aaaTATTTT‑GTGCATAAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa < 1:973193/144‑1 (MQ=255) tgttCT‑TTCCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg < 1:169537/148‑1 (MQ=255) ttCT‑GTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc < 2:131143/150‑1 (MQ=255) ttCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag < 2:1015783/148‑1 (MQ=255) tCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt > 1:474360/1‑151 (MQ=255) ggCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg < 1:354114/141‑1 (MQ=255) | CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCT‑GTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTT > NC_000913/3556051‑3556341 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |