Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC44.6% 66.4 / 21.3 56S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (23/8);  new base C (0/25);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 4.37e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556057‑3556345
                                                                                                                                                        |                                                                                                                                          
gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                            <  1:677640/151‑1 (MQ=255)
  gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcc                                                                                                                                          <  1:744470/151‑1 (MQ=255)
      cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGc                                                                                                                                      <  1:159013/151‑1 (MQ=255)
       aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                     >  2:284919/1‑151 (MQ=255)
                          cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                  >  2:973214/1‑151 (MQ=255)
                          cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                  >  2:937789/1‑151 (MQ=255)
                              tttCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              >  1:547348/1‑151 (MQ=255)
                                tccccaccgaaAAAAAGCCGTTGGGTCATATTTTCCCGTCCGACTCCAAGCGAGCCGTTATTACCCGCCCCCGGGCCGCGCGGCGGTTTATGAATTTTCGTTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                              <  1:761265/142‑1 (MQ=255)
                                 cACCGACAAAAAAAAAGCTTTGGGTAATATTTTCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGCCCCCGGGCCGCGCGGCAGTTTATAAATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa                                                                                                            <  2:358814/150‑1 (MQ=255)
                                       ccaaaaaaaaCCTTTGGGGGATATTTTCCCTTCCGCCTCAAGGCGAAGCGGTTTTCCCCGGCCCGTGGTGCCGGGGCAGGTTAGAATTTTTTGGTGCATGCGGAGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                       <  1:684083/145‑1 (MQ=255)
                                          cAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                  >  1:1019160/1‑151 (MQ=255)
                                            aaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                 >  1:905169/1‑150 (MQ=255)
                                                 gCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                            >  2:898057/1‑150 (MQ=255)
                                                  cGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                            >  2:99538/1‑149 (MQ=255)
                                                   gTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                         >  1:651304/1‑151 (MQ=255)
                                                    ttgggTAATATTTTCACTTTCGCCTCCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGGGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg                                                                                          <  2:635694/146‑1 (MQ=255)
                                                    ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                        >  2:303602/1‑151 (MQ=255)
                                                      cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                       >  2:897420/1‑150 (MQ=255)
                                                       gttGATATTTTCTCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                       <  2:773286/147‑1 (MQ=255)
                                                       ggTGATTTTTTCCCTTCCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                         <  2:13122/147‑1 (MQ=255)
                                                        gtatttttttCACTTTCGACTTCAGGCAAGCCGGTTTCCCCCGGCCCCGGGTCGCGCGGAGGGTTATAAATATTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                      <  2:869424/143‑1 (MQ=255)
                                                            ttttttCTCTTTCGCCTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                   <  2:888057/146‑1 (MQ=255)
                                                            ttttttCACTTGCGACTACAAGCGAGACGGTATTCCCCGCCCCCGGGCCGCGGGGCGGGTTATAAATATTCTTTTCAGGGAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                  <  1:238735/147‑1 (MQ=255)
                                                              ttttCCCTTTCGACTACAAGCGAGACGTTATCCCCCGGCCCCGGGCCGCGCGGCGGGTTATAAATATTTTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                <  2:603108/149‑1 (MQ=255)
                                                              ttttCACTTTCGACTCCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                <  2:518827/149‑1 (MQ=255)
                                                               tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                             >  2:512200/1‑151 (MQ=255)
                                                                ttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                            >  2:350965/1‑151 (MQ=255)
                                                                     tttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                       >  2:562181/1‑151 (MQ=255)
                                                                      ttCGTCTACAAGCGAGTCGTTTTTCCCCGCCCCCTGGCCGCGCGGGAGGTTATGAATTTTCTTTTAATGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                       <  1:831033/150‑1 (MQ=255)
                                                                        cGACTTCAAGCGAGACGTTATCCCCGGCCCCTTGGTCGCGCGGCAGTTTATAATTTTTTTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                       <  1:797829/148‑1 (MQ=255)
                                                                        cGACTCCAAGCGAGACGGTATTCCCCGCCCCCGGGCCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                      <  2:510241/149‑1 (MQ=255)
                                                                              cAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                              >  1:101399/1‑151 (MQ=255)
                                                                               aaGCGAGACGGTATTCCCCGCCCCGTGGGCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                             <  1:167796/151‑1 (MQ=255)
                                                                                   gagaCGTTATTCCCCGCCCCCTGTCCGCGCGGCAGTTTATGAATTTTTTTTTCATGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                           <  1:445317/149‑1 (MQ=255)
                                                                                   gagaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                         >  2:464192/1‑151 (MQ=255)
                                                                                   gagaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                         >  1:653045/1‑151 (MQ=255)
                                                                                          tATTCCCCGCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                    <  1:336425/149‑1 (MQ=255)
                                                                                             tACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                               >  1:538304/1‑151 (MQ=255)
                                                                                              tcccggcacccggcgcgcgcgGGCGGTTATTACTTTTCTGTTCAGGAAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt                                                <  2:72982/135‑1 (MQ=255)
                                                                                              ccccGCCCCCTGGTCGCGGGGCATTTTATAAATTTTCTTTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                              <  1:1024485/150‑1 (MQ=255)
                                                                                               cccGGCCCCTGGTCGCGGGGCAGGTTTTGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                               <  1:299739/149‑1 (MQ=255)
                                                                                               cccGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                             >  2:1007204/1‑151 (MQ=255)
                                                                                               cccGGCCCCTGGGCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                               <  1:978486/149‑1 (MQ=255)
                                                                                                    ccccGGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCa                                           <  1:388357/148‑1 (MQ=255)
                                                                                                       cTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                     >  1:552778/1‑151 (MQ=255)
                                                                                                             cgggcgcaagtttATGAATTTTCTTTTCGTGCAGAAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCaa                                          <  1:31664/130‑1 (MQ=255)
                                                                                                              gcgcgGCAGTTTATAAATTTCCTGTCCATGGGGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgt                                <  1:62794/150‑1 (MQ=255)
                                                                                                              gcgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                               >  1:130083/1‑151 (MQ=255)
                                                                                                                    cAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                         >  1:812972/1‑151 (MQ=255)
                                                                                                                        ttATGAATATTCTGTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      <  2:424323/150‑1 (MQ=255)
                                                                                                                            aaaTATTTTGTGCATAAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       <  1:973193/144‑1 (MQ=255)
                                                                                                                               tgttCTTTCCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               <  1:169537/148‑1 (MQ=255)
                                                                                                                                 ttCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc             <  2:131143/150‑1 (MQ=255)
                                                                                                                                       ttCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag          <  2:1015783/148‑1 (MQ=255)
                                                                                                                                        tCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt      >  1:474360/1‑151 (MQ=255)
                                                                                                                                           ggCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg            <  1:354114/141‑1 (MQ=255)
                                                                                                                                                 aaaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGt  <  1:917553/145‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                          
GTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556057‑3556345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: